1,721,042 research outputs found
Functional differences between dendritic cells derived from CD34+ bone marrow and peripheral blood stem cells
No full textBackground and Objectives. It has been previously demonstrated that dendritic cells (DCs) are characterized by an immature stage with high antigen internalization capacity, followed by a mature stage with predominantly immunostimulatory ability. The shift from the immature to the mature state can be induced in vitro by the addition of tumor necrosis factor-α (TNFα). The aim of our study was to investigate the maturation steps of DCs obtained from CD34+ cells from peripheral blood stem cells (PBSC) and bone marrow (BM). Design and Methods. DCs were generated in vitro from PBSC and BM CD34+ selected cells. The endocytic activity of the cells was measured by means of dextran-FITC uptake and alloreactivity evaluated with mixed leukocyte reactions. Immunophenotypic analysis was performed by flow cytometry. Results. We observed that DCs from PBSC, in contrast to the BM derived DCs, were never able to take up soluble antigens. Mixed leukocyte reaction (MLR) performed both on PBSC and BM CD34+ derived DCs showed an allo-stimulatory activity comparable to normal controls at day 10, but significantly higher at day 14 after the addition of TNFα. Immunophenotypic analysis showed typical dendritic markers in all the samples and, after treatment with TNFα, enhanced expression of co-stimulatory molecules. Interpretation and Conclusions. Our data seem to indicate that, in our culture conditions. BM-derived DCs could be efficiently used for pulsing with specific peptides, while PBSC-derived DC, being functionally mature, should be more suitable for gene therapy. (C) 2000, Ferrata Storti Foundation
Prevenzione delle EST (Encefalopatie spongiformi trasmissibili) con peptidi sintetici: effetti sulla progressione della patologia
No full textRilievo di PrPres nella milza di criceti utilizzati come modello sperimentale per le EST
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Diagnostic exercise : sudden death in a dog after doxorubicin chemotherapy
No full textA case is reported of fatal cardiomyopathy in an 8-year-old female German Shepherd after standard chemotherapy with doxorubicin for splenic hemangiosarcoma. The main gross lesion was a moderate bilateral cardiac ventricular dilation with diffusely pale myocardium. Histological analysis revealed severe multifocal vacuolar degeneration of cardiomyocytes, myocytolysis, myofibril loss, myocardial fibrosis, and edema. Myocardial fiber vacuolization and myocytolysis were highly suggestive of doxorubicin cardiotoxicity
Detection of PrPres in the spleen of hamsters used as an in vivo model for experimental TSE
Full text linkIn order to detect the presence of PrPres in the spleen of infected hamsters, we used a newly developed extraction method, before Western blot analysis, allowing the precipitation of PrPres. The NaPTA treatment applied before proteolysis causes a change in the PK cleavage site, resulting in the generation of larger PrPres fragments, acting on the N-terminal region of PrPres. By using this procedure, with some modifications, we succeeded in detecting the prion protein in the spleen and obtained the expected bands of prion protein, while in previous reports only very weak signals were obtained. Moreover, it was possible to confirm that the amount of detectable PrPres in the hamster spleen is very low, suggesting that the spleen plays a non-essential role for development of the disease in the hamster experimental model
Serological testing to monitor immunity to canine vaccines: an useful tool in veterinary practice
No full textLysozyme Concentrations in Serum and Mammary Secretions of Jennies and in Serum of Donkey Foals : Preliminary Results
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