1,721,016 research outputs found
Different strategies for covalent immobilization of enzymes on hydroxyapatite: a comparative study
No full textImmobilization of enzymes on insoluble supports is a well-established strategy to implement biocatalysis in industrial processes avoiding poor long-term stability and difficult recyclability of enzymes and enabling process intensification via continuous flow technologies. Greener alternatives compared to petrol-based expensive supports usually employed have been subject of investigation in recent years. Among these, hydroxyapatite (HAP) represents a suitable candidate thanks to its structural stability, non- toxicity, large surface area and ease of surface modification. As it can be sourced from waste, it fulfills also the circular economy model. Three model enzymes were chosen to test HAP as a support: a vanadium-dependent chloroperoxidase from Curvularia inaequalis (CiVCPO), a L-tyrosine decarboxylase from Lactobacillus brevis (LbTDC) and an R-selective transaminase from Thermomyces stellatus (TsRTA). The immobilization of these on commercial supports has been reported in the context of producing valuable APIs or natural products. Three different strategies for enzyme immobilization, different experimental conditions, and protein loading were tested to maximize the immobilization yield and the recovered activity (Figure 1). The enzymes were efficiently bound to the support and their recyclability was tested successfully
Biocatalytic synthesis of Profens and development of a new immobilized His-Tag Horse Liver Alcohol dehydrogenase
No full textIn collaboration with the University College of Dublin we are developing a project about the immobilization of HLADH. Enzyme immobilization is an important goal that allows the recovery of the enzyme from the reaction mixture and its recycle. Immobilization can be seen like an environmentally friendly improvement and the final aim is to allow the scale up of the process to the industrial scale
Biotransformations in synthesis of Profens and development of a new immobilized His Tag Horse Liver Alcohol Dehydrogenase
No full textHighly efficient asymmetric reduction of arylpropionic aldehydes by Horse Liver Alcohol Dehydrogenase through dynamic kinetic resolution
No full textA new transaminase from the halophilic bacterium Halomona elongata: Expression, purification and characterization.
No full textAcid hydrolysis of an ether bond assisted by the neighbouring amide group: effects induced by salts and by structural changes part 6.
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