1,422 research outputs found
I mezzi di contrasto intravascolari organo-iodati:caratteristiche chimico-fisiche, indicazioni e controindicazioni
No full textLibro edito per conto della Società Italiana di Radiologia Medic
Fibroblast behavior after titanium surfaces exposure
No full textBackground: The main requirements for a good material are its ability to promote attraction and adhesion of bone precursor cells and their proliferation and differentiation. Different biocompatible materials are currently employed as scaffold. Among these, titanium is considered a "gold standard" because of its biocompatibility and good corrosion resistance.
Materials and Methods: The aim of this work was to compare two different AoN titanium layers (GR4 and GR5) to investigate which one had a greater osteoconductive power using human fibroblasts (HFb) culture at two different time-points. The expression levels of some adhesion and traction-resistance related genes (COL11A1, COL2A1, COL9A1, DSP, ELN, HAS1, and TFRC) were analyzed using real time reverse transcription-polymerase chain reaction.
Results: After 7 days of treatment with TiA 4GR, the only two up-regulated genes were COL2A1 and DSP. After 15 days of treatment, none of genes over expressed.
Conclusion: Our preliminary results suggest that neither AoN 4GR nor AoN 5GR are able to promote the production of protein involved in cell-cell and cell-matrix adhesion and in stress-resistance, required for a good outcome in dental implantology
Titanium alloys (AoN) and their involvement in osseointegration
No full textBackground: Osseointegration is essential for a long-term successful and inflammation-free dental implant. Such a result depends on osteoblastic cells growth and differentiation at the tissue-implant interface. The aim of this study was to compare two different AoN titanium layers (GR4 and GR5) to investigate which one had a greater osteoconductive power using human osteoblasts (HOb) culture at two different time-points.
Materials and Methods: The expression levels of some bone-related (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analyzed using real time reverse transcription-polymerase chain reaction (real time RT-PCR).
Results: Real-time RT-PCR data showed that after 3 days of treatment with TiA4GR, the genes up-regulated were COL3A1, ALPL, SPP1, and RUNX2. Moreover, no difference in gene expression was noticed 4 days later. On the other hand, the genes that overexpressed after 3 days of treatment with AoN5GR were ALPL, SPP1, and RUNX2. In both cases, the expression of COL1A1 and SPARC was negatively regulated.
Conclusion: Our data showed that both titanium surfaces led to osteoblasts recruitment, maturation, and differentiation, thus promoting osseointegration at the tissue-implant interface
The preoperative staging of rectal neoplasms: the clinical exam and diagnostic imaging [Stadiazione preoperatoria delle neoplasie del retto: esame clinico e diagnostico per immagini.]
No full textThe management of rectal cancer remains an important clinical problem. Although there was been great progress in surgical management, the survival of patients with locally advanced disease has not improved significantly during the past decades. Preoperative staging and evaluation of the risk of recurrence may help in the choice of operation. It is difficult for clinicians to quantify reliably with digital examination the degree of fixation of the tumor, and they usually cannot distinguish nodal metastases except in advanced cases. The more frequent overstaging of small tumors within one quadrant of the rectum is a major drawback of digital examination. Computed tomography and magnetic resonance seems to underestimate the extension of rectal tumors, but both can be helpful in selecting patients with advanced tumors for whom preoperative adjuvant treatment is being considered. Endoluminal ultrasound is superior in staging tumors confined to the rectal wall, but is not the ideal tool for staging: the results are examiner dependent, the field of vision in depth is limited, and stricturing tumors cannot be passed by the ultrasound transducer. Imaging diagnostic attendibility confirms the preeminent role of intraoperative exploration in the assessment of neoplastic diffusion in order to plan a correct surgical treatment
Titanium alloys (AoN) and their involvement in osseointegration.
No full textBackground:
Osseointegration is essential for a long-term successful and inflammation-free dental implant. Such a result depends on osteoblastic cells growth and differentiation at the tissue-implant interface. The aim of this study was to compare two different AoN titanium layers (GR4 and GR5) to investigate which one had a greater osteoconductive power using human osteoblasts (HOb) culture at two different time-points.
Materials and Methods:
The expression levels of some bone-related (ALPL, COL1A1, COL3A1, SPP1, RUNX2, and SPARC) were analyzed using real time reverse transcription-polymerase chain reaction (real time RT-PCR).
Results:
Real-time RT-PCR data showed that after 3 days of treatment with TiA4GR, the genes up-regulated were COL3A1, ALPL, SPP1, and RUNX2. Moreover, no difference in gene expression was noticed 4 days later. On the other hand, the genes that overexpressed after 3 days of treatment with AoN5GR were ALPL, SPP1, and RUNX2. In both cases, the expression of COL1A1 and SPARC was negatively regulated.
Conclusion:
Our data showed that both titanium surfaces led to osteoblasts recruitment, maturation, and differentiation, thus promoting osseointegration at the tissue-implant interface
Fibroblast behavior after titanium surfaces exposure.
No full textBackground:
The main requirements for a good material are its ability to promote attraction and adhesion of bone precursor cells and their proliferation and differentiation. Different biocompatible materials are currently employed as scaffold. Among these, titanium is considered a gold standard because of its biocompatibility and good corrosion resistance.
Materials and Methods:
The aim of this work was to compare two different AoN titanium layers (GR4 and GR5) to investigate which one had a greater osteoconductive power using human fibroblasts (HFb) culture at two different time-points. The expression levels of some adhesion and traction-resistance related genes (COL11A1, COL2A1, COL9A1, DSP, ELN, HAS1, and TFRC) were analyzed using real time reverse transcription-polymerase chain reaction.
Results:
After 7 days of treatment with TiA 4GR, the only two up-regulated genes were COL2A1 and DSP. After 15 days of treatment, none of genes over expressed.
Conclusion:
Our preliminary results suggest that neither AoN 4GR nor AoN 5GR are able to promote the production of protein involved in cell–cell and cell–matrix adhesion and in stress-resistance, required for a good outcome in dental implantolog
Synthesis of a chemiluminescent probe useful for the purification of steroid 5α-reductase
No full textSteroid 5α-reductase is a system of two isozymes (5αR-1 and 5αR-2), which is related to several human disease. The synthesis of a chemiluminescent probe (AZA-LU) useful for purification of 5αR is described. AZA-LU has a chemiluminescence detectable up to 10-15 M and is a good inhibitor (IC50 = 0.5 μM) for rat 5αR-1 expressed in transformed Saccharomyces cerevisiae yeast. Incubation of AZA-LU with yeast lysates, followed by SDS-PAGE, led to the detection of two luminescent protein regions with estimated molecular weights of 26 kDa and 21 kDa
Spiral implants bearing full-arch rehabilitation: analysis of clinical outcome
No full textAbstract Background: A spiral implant is a conical internal helix implant with a variable thread design which confers the characteristic of self drilling, self tapping and self bone condensing. Reports have reported the effectiveness of this type of implants in several clinical situations. However, because there are no reports that specifically focus on one of the biggest challenges in oral rehabilitation, i.e. the full arch rehabitation, it was decided to perform a retrospective study.Materials and Methods: The study population was composed of 23 patients (12 females and 11 males, median age 57 years) for evaluation and implant treatment between January 2005 and June 2009. 206 Spiral family implants (SFIs) were inserted with a mean post loading follow-up of 23 months. Several variables were investigated: demographic (age and gender), anatomic (maxilla and mandible, tooth site), implant (type, length and diameter), surgical (surgeon, post-extractive, flapless technique, grafts) and prosthetic (implant/crown ratio, dentition in the antagonist arch, type of loading and CT planning) variables. Implant loss and peri-implant bone resorption were evaluated. Univariate and multivariate test were performed.Results: Survival and success rates were 97.1 and 82.5\%, respectively. Only implant length and implant/crown ratio were statistical significance in determining a better clinical outcome.Conclusion: In conclusion, SFIs are a reliable tool for most difficult cases of oral rehabilitation. No differences were detected among implant type. Length and implant/crown ratio can influence the crestal bone resorption with better result for longer fixtures and an higher implant/crown ratio. In addition banked bone derived from living donors can be used to restore alveolar ridge augmentation without adverse effects. Finally, flapless and CT-planned surgery did not significant increases the clinical outcome in most complex rehabilitation
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