334 research outputs found
Stimolatori biologici per la rigenerazione dei tessuti in ortopedia : indicazioni terapeutiche
No full textCaratterizzazione aromatica di due genotipi di fragole mediante analisi GC-MS e HS-SPME-GC
No full textEvaluation of postharvest quality of strawberries in Italy
No full textA national project to evaluate the quality of strawberry cultivars has been running in Italy for ten years. The aim of the program is to recommend cultivars suited to different growing areas. National and foreign cultivars have been assessed for at least two years for postharvest quality, including a short storage time to simu-late conditions during export. A score of 0 to 1 (0=poor, 0.5=average, and 1=good) was assigned to each quality parameter. In the Cesena area, the cultivars ‘Alba’, ‘Kore’, ‘Cifrance’, ‘Marjolaine’, ‘Queen Elisa’, ‘Idea’ and ‘Nadina’ had the highest scores, while in the Verona area the best were ‘Thethis’, ‘Darselect’, ‘Marmolada®Onebor’ and ‘Miss’. Additionally at harvest time, a sensory test was performed on some cultivars from both areas for sweetness, acidity, aroma and firmness. In the Cesena area, ‘Dora’ was judged sweet, firm and aromatic, followed by ‘Queen Elisa’. In Verona, ‘Darselect’ had the highest aroma leve
Recupero della vecchia cv di melo Renetta ruggine di Torriana : caratterizzazione e problemi di conservabilità
No full textTrials were carried out on the old apple cv Renetta ruggine di Torriana, evaluating fruit quality and keeping quality. The storage was performed at 2 deg C both in air and in CA (1.5% O2 and 1% CO2) up to 4/6 months. The results showed a high level of internal browning, with no relation with harvesting time. In order to verify if damages were due to low temperature, new storage trials were performed at 0, 2, 4, 6 deg C in air or at 4 deg C in CA. The results showed less internal browning at temperature higher than 4 deg C
Aspetti nutraceutici delle fragole : indagini sul contenuto in acido ascorbico
No full textTwenty fruit samples of 36 cvs and 16 selections grown at Cesena, Boves and Verona from 1994 to 2001 were assayed for the ascorbic acid content. Results showed a wide range of ascorbic acid content, from 20 to 100 mg/100 g f.w., according to the cultivars. Factors such as growing location, year of production and short term storage were also studied, showing large effects on ascorbic acid content
Applicazioni di test sensoriali nella valutazione della qualità organolettica delle fragole
No full textRegulation of NMDA receptor subunit messenger RNA levels in the rat brain following acute and chronic exposure to antipsychotic drugs.
No full textBased on anatomical and biochemical observations a role of glutamate in schizophrenia has been postulated. In the present work we
have investigated the gene expression for two families of NMDA receptor subunits ŽNR-1 and NR-2. following acute and chronic
treatment with typical Žhaloperidol. and atypical Žclozapine. antipsychotic drug ŽAPD. in rats. A single injection of the two drugs elicited
a significant increase in the mRNA levels of NR-2B in the nucleus accumbens, whereas only haloperidol was able to elevate NR-2A and
NR-2B in the hippocampus. Following a 21 day treatment, significant differences in the regulatory pattern of NMDA-R subunits were
observed. Haloperidol increased their mRNA levels in striatum whereas clozapine, consistent with its relatively weaker influence on
nigro-striatal dopamine function, did not change the expression of NR subunits in this region. Both APD’s were able to decrease the
expression of NR-2 subunits in the hypothalamus, but only clozapine was capable of reducing NR-2C in frontal cortex and accumbens.
The regulation of NMDA-R subunits in specific brain regions may represent a novel and important mechanism through which APD’s
exert some of their effects on brain function
Efficacy of tuohy needle in oocytes collection from excised mare ovaries
No full textOocyte aspiration from equine follicles gives a low recovery rate and yields oocytes largely denuded of cumulus cells. Follicle scraping is labour intensive and increases the time required for collection,
extending the holding time of oocytes that delays their maturation.
The aim of this work was to develop an effective method for collecting equine oocytes combining the feature of aspiration (fastness) with that of scraping (high recovery rate of cumulus-intact oocytes). Furthermore, we examined differences in cumulus morphology and maturation rates, comparing this technique to aspiration and scraping, with or without tunica albuginea removal. Collection by vacuum pump aspiration was performed using a 16 g needle while the combination of aspiration and scraping was performed using a Tuohy needle (16 g) that is usually employed for inserting an epidural catheter and its tip shape is similar to a small curette. In unpeeled ovaries, the recovery rates by the Tuohy needle was higher (p < 0.05) than in the 16 g needle aspiration and in the scraped ovaries (57% vs. 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% vs. 39.36%) but lower than scraping (46.97%) (p < 0.001). In unpeeled ovaries there was no difference in maturation rate of oocytes recovered by Tuohy needle in respect to scraping in peeled ovaries (58.54% vs. 58.24% respectively; p < 0.05). In conclusion, combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM
y-conglutin, the lupin seed-glucose lowering glycoprotein, interferes on insulin pathway in HepG2 cells
No full textGamma-conglutin (γ-C), a Lupinus albus, seed glycoprotein, has been shown to lower blood glucose in hyperglycaemic rats and increase glucose consumption in HepG2 cells. Aim of the present work was to assess the modalities of interaction of this protein with the target cells, so as to contribute unveiling its mechanism of action. γ-Conglutin internalization: HepG2 cells, pretreated for 30’ w/wo inhibitors of caveolae mediated endocytosis [filipin (5 μg/ml) and genistein (200 μM)], clathrin-mediated pathway [chlorpromazine (25 μM), methyl-β-cyclodextrin (5 mM)], and macropinocytosis [amiloride (5 mM)], were incubated with FICT-γ-conglutin (50 μg/ml) for 4 h at 37°C, followed by FACS analysis. The macropinocitosis pathway was the mechanism of γ-conglutin internalization (69% inhibition). 2D-electrophoresis of cell lysates and antibody reaction of the blotted maps showed that γ-conglutin is internalized in intact form. Moreover, the protein is modified inside the cell by multiple phosphorylation. Insulin pathway: reduced effect on AMPK activity (-50% vs controls) and increased expression of phosphorylated AKT form (+ 25% vs controls) were recorded in HepG2 cells, following western-blot analysis, exposed to 10 mM γ-conglutin and/or 100 nM insulin, or metformin 10 mM
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