1,721,136 research outputs found
Acute promyelocytic leukemia: a curable disease
No full textThe Second International Symposium on Acute Promyelocytic Leukemia (APL) was held in Rome in 12-14 November 1997. Clinical and basic investigators had the opportunity to discuss in this meeting the important advances in the biology and treatment of this disease achieved in the last 4 years, since the First Roman Symposium was held in 1993. The first part of the meeting was dedicated to relevant aspects of laboratory research, and included the following topics: molecular mechanisms of leukemogenesis and of response/resistance to retinoids, biologic and therapeutic effects of new agents such as arsenicals and novel synthetic retinoids; characterization of APL heterogeneity at the morphological, cytogenetic and immunophenotypic level. The updated results of large cooperative clinical trials using variable combinations of all-trans retinoic acid (ATRA) and chemotherapy were presented by the respective group chairmen, and formed the 'core' part of the meeting. These studies, which in most cases integrated the molecular assessment of response to treatment, provided a stimulating framework for an intense debate on the most appropriate frontline treatment options to be adopted in the future. The last day was dedicated to special entities such as APL in the elderly and in the child, as well as the role of bone marrow transplantation. The prognostic value of molecular monitoring studies was also discussed in the final session of the meeting. In this article, we review the major advances and controversial issues in APL biology and treatment discussed in this symposium and emerging from very recent publications. We would like to credit the successful outcome of this meeting to the active and generous input of all invited speakers and to participants from all over the world who provided constructive and fruitful discussions
Determination of cadmium and lead in fruit juices by stripping chronopotentiometry and comparison of two sample pretreatment procedures
No full textCampionamento e valutazione analitica del grado di inquinamento da idrocarburi policiclici aromatici di un sito industriale dismesso deposito di coke di petrolio
No full textConfronto di due tecniche per l'estrazioni di idrocarburi policiclici aromatici in campioni a diverso grado di inquinamento: determinazione LC-FD
No full textGli Idrocarburi Policiclici Aromatici (IPA) rappresentano una delle classi di composti il cui monitoraggio in matrici ambientali ed alimentari è di fondamentale importanza a causa del loro potere cancerogeno e mutagenico. Gli IPA sono composti da due o più anelli benzenici condensati che si formano durante processi di combustione incompleta ad alte temperature di materiale carbonioso; in ragione della loro natura idrofobica e della loro bassa solubilità tendono ad accumularsi nel particolato atmosferico che può essere diffuso in tutto l’ecosistema. Data la loro azione nociva, queste sostanze sono state incluse nella lista degli inquinanti principali dell’Unione Europea (UE) e dell’Environmental Protection Agency (EPA). Le acque reflue ricevono elevate quantità di IPA da diverse fonti, quali: immissioni industriali e domestiche da fonti fossili e acque urbane che dilavano questi composti depositati al suolo dai veicoli e/o dagli impianti di riscaldamento. Il problema principale riguardo l’estrazione e la determinazione degli IPA si riferisce ai bassi limiti di sensibilità richiesti ed alla presenza di potenziali interferenti all’interno di matrici complesse. A tal proposito sono state applicate diverse tecniche di estrazione, quali: soxhlet, estrazione liquido-liquido, sonicazione, estrazione in fase supercritica, estrazione con microonde. Inoltre sono state sviluppate differenti tecniche cromatografiche in grado di determinare gli IPA in modo selettivo sfruttando la loro capacità di separazione, soprattutto quando accoppiate a detector selettivi. In questo lavoro sono stati determinati i 16 idrocarburi policiclici aromatici inseriti nella lista degli inquinanti prioritari dell’EPA in campioni di percolati (3) e di acque reflue (3) provenienti da un impianto di rifiuti solidi urbani della provincia di Roma. L’estrazione è stata ottenuta mediante sonicazione e tecnica a microonde per i campioni di percolato ed estrazione liquido-liquido e tecnica a microonde per i campioni di acque reflue; la determinazione quantitativa è stata effettuata mediante cromatografia liquida con rivelatori UV e fluorimetrico in serie
Determinazione di idrocarburi policiclici aromatici in campioni di acqua transfrontalieri prelevati nel torrente Corno e nel fiume Isonzo mediante gascromatografia ad alta risoluzione accoppiata alla spettrometria di massa a bassa risoluzione
No full textIn questo lavoro si è voluto valutare l'eventuale inquinamento da idrocarburi policiclici aromatici in
campioni di acque transfrontalieri, relativamente ai 16 IPA inseriti nella lista degli inquinanti prioritari
dell’EPA, effettuando 6 opportuni campionamenti nel fiume Isonzo e nel torrente Corno, secondo le
metodologie ufficiali di campionamento. L’estrazione è stata effettuata mediante microestrazione in
fase solida e la determinazione è stata fatta mediante gascromatografia ad alta risoluzione accoppiata
alla spettrometria di massa
Terminal deoxynucleotidil transferase (TdT) activity in human leukemia. Quantitative evaluation with enzymatic immunoassay (EIA).
No full textMetodologie analitiche di campionamento e determinazione di metalli pesantinelle emulsioni oleose utilizzati in co-combustione nei forni da cemento
No full textMetodologie analitiche di campionamento e determinazione di metalli pesanti nelle emulsioni oleose utilizzate in co-combustione nei forni da cemento
No full textBiogenic amines in wine: occurrence and influence on wine aroma
No full textAroma is a highly important aspect determining the quality of wine. Primary aromas are those belonging to and characteristic of the variety of grape used for the elaboration of the wine. The compounds originating from fermentation – the most abundant – are responsible for the fruity and/or flowery aromas of wine and are known as secondary aromas. Finally, the compounds giving rise to the tertiary aromas come from the oak wood during the ageing process in the cask and subsequent evolution in the bottle.
To this regard, the presence of amines in wine in high concentrations, can produce a negative impact on the aroma of the product and, in some cases, detrimental health effects. Several factors influence the content of amines in wine, among them the yeast strain and the amino acid composition of the must. Among these amines, histamine is the most toxic and is known to cause headaches and low blood pressure. Tyramine and phenylethylamine can produce hypertension. Putrescine and cadaverine, although not toxic in themselves, intensify the adverse effects of the abovementioned amines because they interfere with the enzymes that metabolize them. Volatile amines do not have a toxic action on the human organism, but they can have a negative effect on wine aroma. Biogenic amines and phenylethylamine, which is a volatile amine, have their origin in the microbial decarboxylation of amino acids. Although the origin of volatile amines has not been fully studied, it is thought that they probably come from amination of non-nitrogen compounds such as aldehydes and ketones.
These nitrogenated compounds are mainly formed during the alcoholic and malolactic fermentations of the wine, their importance lying in the negative effects on human health that can arise with their ingestion.
These findings are important in that they warn the enologist not to drop their guard in the control of these post-malolactic fermentation compounds, especially if part of the wine production is to be exported. Histamine, tyramine and putrescine are the 3 biogenic amines who can develop in substantial amounts during and after malolactic fermentation (MLF).
The study deals with the chromatographic determination of histamine, tyramine, putrescine and cadaverine and their possible relation with wine organoleptic characteristics
Kaposi's sarcoma following malignant mesothelioma.
No full textWe report the unusual occurrence of Kaposi's sarcoma following asbestos-related malignant mesothelioma, in a human deficiency virus (HIV)-negative Italian man. Seropositivity to human herpes virus 8 (HHV8) was documented at the time of mesothelioma diagnosis and preceded the onset of Kaposi' sarcoma with a time lapse of 13 months. HHV8 DNA was detected by polymerase chain reaction in lesional Kaposi's sarcoma but not within mesothelioma. By immunostaining, mesothelioma cells expressed interleukin-6 and platelet-derived growth factor, which are important for survival of Kaposi's sarcoma cells. Besides the possibility of a casual association, we hypothesize that mesothelioma-linked factors may have contributed to the development of Kaposi'sarcoma in the presence of HHV8 infection
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