250 research outputs found
Beneficio clinico di lunga durata in paziente con carcinoma mammario metastatico HER2-positivo trattato con lapatinib e capecitabina
No full textSupratentorial High-Grade Gliomas: Maximal Safe Anatomical Resection Guided by Augmented Reality High-Definition Fiber Tractography
No full textObjectives:
In this study, the authors aimed to evaluate the safety and efficacy profiles of HDFT-F microscope- based AR cytoreductive surgery for newly diagnosed supratentorial HGGs.
Background: The theoretical advantages of augmented reality (AR) with diffusion tensor imaging (DTI)–based high- definition fiber tractography (HDFT) and sodium fluorescein (F) in high-grade glioma (HGG) surgery have not been investigated in detail.
Methods:
Data of patients with newly diagnosed supratentorial HGGs who underwent surgery using the AR HDFT-
F technique were reviewed and compared with those of a cohort of patients who underwent conventional white-light surgery assisted by infrared neuronavigation. The safety and efficacy of the techniques were reported based on the postoperative Neurological Assessment in Neuro-Oncology (NANO) scores, extent of resection (EOR), and Kaplan-Mei- er curves, respectively. The chi-square test was conducted for categorical variables. A p value < 0.05 was considered statistically significant.
Results:
A total of 54 patients underwent surgery using the AR HDFT-F technique, and 63 underwent conventional white-light surgery assisted by infrared neuronavigation. The mean postoperative NANO scores were 3.8 ± 2 and 5.2 ± 4 in the AR HDFT-F group and control group, respectively (p < 0.05). The EOR was higher in the AR HDFT-F group (p
< 0.05) than in the control group. With a mean follow-up of 12.2 months, the rate of progression-free survival (PFS) was longer in the study group (log-rank test, p = 0.006) than in the control group. Moreover, the complication rates were 9.2% and 9.5% in the study and control groups, respectively.
Conclusions:
Overall, AR HDFT-F–assisted surgery is safe and effective in maximizing the EOR and PFS rate for patients with newly diagnosed supratentorial HGGs, and in optimizing patient functional outcomes
Eribulin in male patients with metastatic brest cancer: first report of clinical outcome
No full textMicroscope-based augmented reality with diffusion tensor imaging and fluorescein in insular glioma resection
No full textMaximal safe resection is the goal of insular glioma surgery. The combination of intraoperative augmented reality (AR) diffusion tensor imaging (DTI) fiber tracking with fluorescein dye (F) helps achieve this goal throughout a microscope-based visualization of the tumor and white matter fiber tracts. The aim of the present video article was to show the technical key aspects of DTI-F microscope-based AR-assisted surgery during the gross-total resection of an insular Berger-Sanai type I+IV high-grade glioma in a 63-year-old patient, performed through a pterional transsylvian approach. The video can be found here: https://stream.cadmore.media/r10.3171/2021.10.FOCVID2157
pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers
No full textIsolated and purified reaction centers (RC) from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed
Pretreatment normalization of mild anemia with epoietin alfa: impact on the outcome in epithelial ovarian cancer
No full textHeavy metal ion influence on the photosynthetic growth of Rhodobacter sphaeroides
No full textThe potential of purple non-sulphur bacteria for bioremediation was assessed by investigating the ability of Rhodobacter sphaeroides strain R26.1 to grow photosynthetically in heavy metal contaminated environments. Bacterial cultures were carried out in artificially polluted media, enriched with the transition metal ions Hg2+, Cu2+, Fe2+, Ni2+, Co2+, MoO42-, and CrO42- in millimolar concentration range. For each investigated ion the effect on growth parameters was evaluated. The analysis of concentration-effect curves revealed a differentiated response, indicating that diverse mechanisms of tolerance and/or resistance are involved. Adaptation or selection procedures were not applied, leading to assess intrinsic abilities of coping with these contaminants. The microorganism proved to be highly tolerant to heavy metal exposure, especially towards Co2+, Fe2+ and MoO42-. In addition Ni2+ and Co2+ were found to decrease the cellular content of light harvesting complexes. A characteristic behavior was observed with mercuric ions, which produced a significant increase of the lag-phase. (c) 2005 Elsevier Ltd. All rights reserved
Heavy metal ion influence on the photosynthetic growth of Rhodobacter sphaeroides
No full textThe potential of purple non-sulphur bacteria for bioremediation was assessed by investigating the ability of Rhodobacter sphaeroides strain R26.1 to grow photosynthetically in heavy metal contaminated environments. Bacterial cultures were carried out in artificially polluted media, enriched with the transition metal ions Hg2+, Cu2+, Fe2+, Ni2+, Co2+, MoO42-, and CrO42- in millimolar concentration range. For each investigated ion the effect on growth parameters was evaluated. The analysis of concentration-effect curves revealed a differentiated response, indicating that diverse mechanisms of tolerance and/or resistance are involved. Adaptation or selection procedures were not applied, leading to assess intrinsic abilities of coping with these contaminants. The microorganism proved to be highly tolerant to heavy metal exposure, especially towards Co2+, Fe2+ and MoO42-. In addition Ni2+ and Co2+ were found to decrease the cellular content of light harvesting complexes. A characteristic behavior was observed with mercuric ions, which produced a significant increase of the lag-phase. (c) 2005 Elsevier Ltd. All rights reserved
Intraoperative Augmented Reality High-Definition Fiber Tractography for High-Grade Gliomas of The Primary Motor Area
No full textIntroduction
The theoretical advantages of augmented reality (AR) with diffusion tensor imaging (DTI) based high-definition fiber tractography (HDFT) in high-grade glioma (HGG) surgery have not been investigated in detail.
Objectives
The present study aimed to evaluate the safety and efficacy profiles of HDFT-F microscope-based AR cytoreductive surgery for newly diagnosed HGGs involving the primary motor area.
Methods
A consecutive institutional series of patients with newly diagnosed HGGs of the central lobe that were operated on using the AR HDFT technique were reviewed and compared with that of a cohort of patients who underwent conventional white-light surgery assisted by infrared neuronavigation. The safety and efficacy of the technique were reported based on the postoperative Neurologic Assessment in Neuro-Oncology (NANO) scores, the extent of resection (EOR), and the Kaplan–Meier curves, respectively. A chi-squared test was conducted for categorical variables. A p-value < 0.05 was considered statistically significant.
Results
A total of 11 patients were operated on using the AR HDFT-F technique, and 9 underwent conventional white-light surgery. The average postoperative NANO scores were 5.4 ± 2 and 5.7 ± 3 in the AR HDFT-F and control group, respectively. The EOR was higher in the AR HDFT group than in the control group. On an average follow-up of 10.9 months, the rate of progression-free survival (PFS) was longer in the study group than in the control group (log-rank p = 0.045).
Conclusions
AR HDFT assisted surgery is safe and effective in maximizing the EOR and PFS rate, as well as in optimizing the patient’s functional outcomes, of newly diagnosed HGGs of the primary motor area
PH dependence of the charge recombination kinetics in bacterial RC reconstituted in liposomes
No full textThe photosynthetic Reaction Center from the carotenoidless mutant strain of the purple non sulphur bacterium Rhodobacter (R.) sphaeroides was reconstituted in artificial phospholipid vesicles (liposomes) to mimic the physiological membrane environment. The pH dependence in the interval 5-10 of the rate of the charge-recombination reactions from the final electron acceptors QA and QB to the primary electron donor (namely kAD and kBD) have been investigated. The liposomes were constituted of either the zwitterionic phosphatidylcholine (PC) or the negatively charged phosphatidylglycerol (PG), two of the main phospholipids found in the photosynthetic membrane of the bacterium. In both cases, the kAD has no pH dependence similarly to the detergent case. The kBD also has a pH dependence similar to the detergent case, having two distinct regions below pH 7 and above pH 9. Fitting of the titration curve to a function involving two protonation sites results in a marked shift of the pKAs between the different solubilizing environments. These differences are discussed in the frame of possible physiological implications
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