1,689 research outputs found
The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration
No full textHydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various experimental tests are typically performed to assess these materials' ability to promote angiogenesis, re-epithelialization, and the production and maturation of new extracellular matrix. Immunohistochemistry is important in this context because it allows for the visualization of in situ target tissue factors involved in the various stages of wound healing using antibodies labelled with specific markers detectable with different microscopy techniques. This review provides an overview of the various immunohistochemical techniques that have been used in recent years to investigate the efficacy of various types of hydrogels in assisting skin healing processes. The large number of scientific articles published demonstrates immunohistochemistry's significant contribution to the development of engineered biomaterials suitable for treating skin injuries
Faust und Gretchen / nach Carton von F. Rothbart. Gest. von C. Deis
No full textFAUST UND GRETCHEN / NACH CARTON VON F. ROTHBART. GEST. VON C. DEIS
Faust und Gretchen / nach Carton von F. Rothbart. Gest. von C. Deis (1)
Illustration: Faust und Gretchen. (1
Faust und Gretchen / nach Carton von F. Rothbart. Gest. von C. Deis
No full textFAUST UND GRETCHEN / NACH CARTON VON F. ROTHBART. GEST. VON C. DEIS
Faust und Gretchen / nach Carton von F. Rothbart. Gest. von C. Deis (1)
Faust und Gretchen (1
Electron microscopy to characterise nanoparticles in the biological environment
No full textNowadays the use of nanomaterials has led a growing interest in biomedical application as drug delivery systems for the treatment and the diagnosis of different pathologies. Different analytical techniques have been applied to characterise nanoparticles in the biological environment. However, in the attempt to describe in detail the interaction of NPs with the living systems and to detect the possible occurrence of cell damage or death, electron microscopies proved to be especially suitable and actually are irreplaceable techniques thanks to their image resolution at the nanoscale. In this review article, the attention will first be focused on the influence of features on their interaction with tissues and cells; then, the advantages and limits of transmission and scanning electron microscopy to evaluate the suitability of nanovectors as drug-delivery systems will be discussed
In Vitro Models of Biological Barriers for Nanomedical Research
No full textNanoconstructs developed for biomedical purposes must overcome diverse biological barriers before reaching the target where playing their therapeutic or diagnostic function. In vivo models are very complex and unsuitable to distinguish the roles plaid by the multiple biological barriers on nanoparticle biodistribution and effect; in addition, they are costly, time-consuming and subject to strict ethical regulation. For these reasons, simplified in vitro models are preferred, at least for the earlier phases of the nanoconstruct development. Many in vitro models have therefore been set up. Each model has its own pros and cons: conventional 2D cell cultures are simple and cost-effective, but the information remains limited to single cells; cell monolayers allow the formation of cell–cell junctions and the assessment of nanoparticle translocation across structured barriers but they lack three-dimensionality; 3D cell culture systems are more appropriate to test in vitro nanoparticle biodistribution but they are static; finally, bioreactors and microfluidic devices can mimicking the physiological flow occurring in vivo thus providing in vitro biological barrier models suitable to reliably assess nanoparticles relocation. In this evolving context, the present review provides an overview of the most representative and performing in vitro models of biological barriers set up for nanomedical research
Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine
No full textNanoconstructs intended to be used as biomedical tool must be assessed for their capability to cross biological barriers. However, studying in vivo the permeability of biological barriers to nanoparticles is quite difficult due to the many structural and functional factors involved. Therefore, the in vitro modeling of biological barriers -2D cell monocultures, 2D/3D cell co-cultures, microfluidic devices- is gaining more and more relevance in nanomedical research. Microscopy techniques play a crucial role in these studies, as they allow both visualizing nanoparticles inside the biological barrier and evaluating their impact on the barrier components. This paper provides an overview of the various microscopical approaches used to investigate nanoparticle translocation through in vitro biological barrier models. The high number of scientific articles reported highlights the great contribution of the morphological and histochemical approach to the knowledge of the dynamic interactions between nanoconstructs and the living environment
Genève : jardin zoologique : plan général
No full textF. GindrozLithographie1 carton en haut à gauche: "Plan de situation
Deterministic pushdown automata can compress some normal sequences
No full textIn this paper, we give a deterministic pushdown transducer and a normal sequence of digits compressed by it. This solves positively a question left open in a previous paper by V. Becher, P. A. Heiber and the first author
ZANFERRARI, A.; BOLLETTINARI, G.; CAROBENE, L.; CARTON, A.; CARULLI, G.B.; CASTALDINI, D.; CAVALLIN, A.; PANIZZA, M.; PELLEGRINI, G.B.; PIANETTI, F.; SAURO, U. ;
No full textNel lavoro sono sintetizzati ed elaborati, con modifiche, reinterpretazioni e aggiornamenti, i documenti prodotti dai ricercatori del Sottoprogetto "Neotettonica" (Progetto Finalizzato "Geodinamica" del C.N.R.) che hanno operato nell'Italia Nord Orientale dal 1977 al 198
- …
