1,062 research outputs found
Studies on obesity-related dysfunction in insulin-target tissues : insulin receptor isoforms and intraocular in vivo liver imaging
No full textObesity, caused by poor dietary habits and sedentary lifestyle, has brought on a global health crisis. This condition affects over one-third of adults and largely increases the risk of developing metabolic diseases, such as type 2 diabetes and steatotic liver disease. Insulin is a central hormone in regulating metabolism and insulin receptors are ubiquitously expressed. In mammals, the insulin receptor (IR) has two isoforms, IR-A and IR-B, which differ in structure and function and trigger different signalling pathways within the cell. The first half of this thesis focuses on studying the IR isoforms and their role in metabolic dysfunction. Insulin resistance has been associated with variations in IR isoform expression, however, the mechanisms behind tissue/cell type-specific changes in metabolic disease are poorly understood. Using mouse models of obesity/diabetes we report IR isoform expression patterns in different tissues. We further investigated a shift in IR isoform ratio in perigonadal adipose tissue, and found tissue remodeling and immune cells infiltration to be responsible, rather than a change in IR isoform expression in adipocytes. We also worked to identify novel and isoform-specific IR interaction partners, to understand more about the intracellular signalling triggered by the receptors. The second half of this thesis focusses on the liver, a key player in metabolic regulation. The liver is inaccessible for optical imaging and there is a lack of high-resolution non-invasive imaging techniques. Addressing this need, we developed a novel in vivo imaging platform to monitor liver function longitudinally at cellular resolution. We use the anterior chamber of the mouse eye as a transplantation site for liver spheroids, which engraft on the iris. The cornea acts as a natural body window and allows repeated imaging of the same cells over time. We show that the liver spheroids in the eye retain hepatocyte-specific and liver-like features and perform typical hepatic functions. Importantly, we show that in feeding graft-bearing animals an obesogenic diet, the intraocular grafts developed hepatosteatosis, thereby reporting on endogenous liver function. Thus, we foresee this new technology could provide a unique tool to study steatotic liver disease in both basic and pre-clinical settings.List of scientific papersI. Moruzzi, N., Lazzeri-Barcelo, F., Valladolid-Acebes, I., Moede, T., Paschen, M., Leibiger, B., Berggren, P. O., & Leibiger, I. B. (2021). Tissue-specific expression of insulin receptor isoforms in obesity/type 2 diabetes mouse models. Journal of cellular and molecular medicine. 25(10), 4800–4813. https://doi.org/10.1111/jcmm.16452 II. Lazzeri-Barcelo, F., Leibiger, B., Beusch, C. M., Sabatier, P., Zubarev, R. A., Leibiger, I. B., Berggren, P. O., & Moruzzi, N. BioID-mediated identification of novel IR interaction partners. [Manuscript]III. Lazzeri-Barcelo, F., Oliva-Vilarnau, N., Baniol, M.,Leibiger, B., Bergmann, O., Lauschke, V. M., Leibiger, I. B., Moruzzi, N., & Berggren, P. O. (2024). Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function. Nature communications. 15(1), 767. https://doi.org/10.1038/s41467-024-45122-4 IV. Lazzeri-Barcelo, F., Ciardo, P., Leibiger, B., Leibiger, I. B., Berggren, P. O., & Moruzzi, N. (2024). In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye. J Vis Exp. (205), e66234. https://doi.org/10.3791/66234 </p
Taylor, M. D., Klaine, S. J., Carvalho, F. P., Barcelo, D. & Everaarts, J. (eds). — Pesticide residues in coastal tropical ecosystems. Distribution, fate and effects. Taylor & Francis, London & New York. 2003
No full textErard Christian. Taylor, M. D., Klaine, S. J., Carvalho, F. P., Barcelo, D. & Everaarts, J. (eds). — Pesticide residues in coastal tropical ecosystems. Distribution, fate and effects. Taylor & Francis, London & New York. 2003. In: Revue d'Écologie (La Terre et La Vie), tome 60, n°2, 2005. p. 191
Post-mortem toxicology in the diagnosis of sudden death in young and middle-aged victims
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The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of alpha-3',4'-anhydrovinblastine in Catharanthus roseus (L)
No full textTraffic and QoS Management in Wireless Multimedia Networks
No full textThe proceedings contain 6 papers. The topics discussed include: the research agenda of the COST 290; packet scheduling and congestion control; performance evaluation and traffic modeling; mobility support; multihop wireless networks; and services, optimization, and economic aspects
Ecotoxicity of basil (Ocimum Basilicum) extract in aquaculture feeds: Is it really eco-safe for the aquatic environment?
Get PDFPlant extract and essential oils are gaining application in aquaculture, but data about their environmental impact are limited and their potential effects on aquatic organisms are largely unknown. For this study, ecotoxicity tests were performed under standardized conditions on fish feed supplemented with 3 % w/w of a basil supercritical extract (F1-BEO; substance A), F1-BEO extract (substance B), and fish feed without F1-BEO extract (substance C) on three model species of different trophic levels (bacteria, primary producer, primary consumer) considered representative for freshwater (Aliivibrio fischeri, Raphidocelis subcapitata, Daphnia magna) and marine (A. fischeri, Phaeodactylum tricornutum, Paracentrotus lividus) ecosystems. Ecotoxicological response was largely comparable within the same trophic level (whichever the ecosystem). EC50 was not calculable in the concentration range here tested (3.9–500 mg/L) for freshwater and marine microalgae, suggesting that none of the substances were toxic for primary producers. Reduction of A. fischeri bioluminescence at the tested concentration (0.5–10 mg/L) was observed only for substance A (EC50 9.53 mg/L and 9 mg/L for freshwater and marine ecosystems, respectively). Notably, in P. lividus embryotoxicity was higher for substances A (EC50 1.80 mg/L) and C (EC50 4.6 mg/L) than for substance B (EC50 7.10 mg/L), suggesting a toxic effect due to feed dissolution. In contrast, substance B was more toxic (EC50 0.34 mg/L) in D. magna than substances A (EC50 3.98 mg/L) and C (EC50 5.50 mg/L). Based on the Globally Harmonized System of Classification and Labelling of Chemicals, all substances were categorized Acute 2, except for substance A which was categorized Acute 1 for D. magna. Overall, the substances were found to be potentially toxic for an aquatic ecosystem, especially for primary consumer. Further study of plant extract and essential oils is needed to better understand their effects and fate on the aquatic environment
Mussel watch program for microplastics in the Mediterranean sea: Identification of biomarkers of exposure using Mytilus galloprovincialis
Get PDFMicroplastics (MPs) are ubiquitous pollutants that have also been detected in the aquatic ecosystems at high concentrations. The use of shellfish as bioindicators is widespread for assessing and monitoring the environmental quality in both freshwater and marine environments. On this path, biomarkers represent an effective tool in monitoring programs. This minireview would broaden the existing knowledge on biomarkers of MPs in the Mediterranean mussel Mytilus galloprovincialis. This species was selected as it is widely distributed across the Mediterranean Sea and used as a bioindicator to monitor the presence of MPs in the marine environment. The literature search returned only 11 studies, mainly related to oxidative stress biomarkers. Although certain biomarkers were explored to estimate the effects of MPs on M. galloprovincialis, a battery of standardized and validated biomarkers as well as the inclusion of new ones are needed in future studies to obtain more comparable and robust findings across the Mediterranean Sea
The Research Agenda of the COST 290
No full textThe COopération Scientifique et Technique (European Cooperation in Science and Technology) (COST) 290 Action was initiated in 2002 by a small team of researchers at a time when multimedia services were not yet very strongly manifested in the wireless environment; moreover, wireless networks of different scales offering broadband throughput were not so widely deployed and easily accessed. However, the strategic agenda of the COST 290 proposed back in 2002 today appears viable and feasible, still promising significant achievements in the future. Nevertheless, the original agenda can be easily extended further to cover new research topics of great significance. In 4 years, the Action became much greater in the scope of involvement - more than 60 institutions from 25 countries involved - with quite strong involvement of the European research community
Diseño de un elevador de bandejas industrial
No full textHurtado Barcelo, F.; Ribera Navarro, A. (2013). Diseño de un elevador de bandejas industrial. https://riunet.upv.es/handle/10251/36551.Archivo delegad
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