22 research outputs found
PENGARUH KEDISIPLINAN DAN MOTIVASI KERJA TERHADAP KINERJA PEGAWAI (STUDI PADA DINAS TENAGA KERJA PEMERINTAH KABUPATEN GRESIK)
No full textKedisiplinan kerja dan motivasi kerja yang baik sangat menentukan dalam proses Kinerja kerja yang tujuannya untuk meningkatkan kinerja pegawai dalam menjalankan tugas yang dibebankan kepada masing-masing pegawai. Pada penelitian ini obyek yang diambil adalah pada Dinas Tenaga Kerja Pemerintahan Kabupaten Gresik, sumber data meliputi data primer dan sekunder dengan variabel independent Disiplin Kerja (X1), Motivasi Kerja (X2) dengan variabel dependent Kinerja Kerja. Populasi dalam penelitian ini adalah 41 pegawai. Sedangkan sampel yang diambil adalah 5% dari tabel krejcie yaitu 36 Pegawai. Teknik analisis datanya adalah teknik regresi linier berganda, Uji t, Uji F, dan Uji Dominan.
Hasil analisis dengan alat bantu statistik program SPSS ver. 15.0 diperoleh Hasil persamaan regresi linier berganda penelitian ini adalah Y=8,829+0,315X1+0,597X2+e. Berdasarkan hasil penelitian menunjukkan bahwa hasil variabel disiplin kerja terhadap Kinerja kerja maka di dapat t hitung > t tabel atau 0,602 t tabel atau 2,189 > 2,0345 sehingga H0 ditolak dan H1 diterima berarti motivasi kerja berpengaruh secara signifikan terhadap Kinerja kerja pegawai. Sedangkan secara simulta (bersama-sama) tidak pengaruh kedisiplinan kerja dan motivasi kerja (F hitung > F tabel atau 2,736 < 3,28). Adjusted R Square = 0,142 dapat dikatakan bahwa perubahan variabel terikat (Y) sebesar 21,4% terhadap variabel X1 dan X2, sedangkan sisanya 78,4% disebabkan oleh faktor lain yang tidak ada dalam model ini.
Dari hasil uji dominan disimpulkan bahwa motivasi kerja lebih dominan terhadap Kinerja kerja dibanding kedisiplinan kerja. Diperoleh dengan hasil motivasi kerja 0,355 dan kedisiplinan kerja 0,097
Morphology of <i>H. pylori</i> cells in mono and co-culture.
No full text<p><i>H. pylori</i> NCTC 11637 cells in monoculture ((a), (c), (e) and (g)) and in co-culture with <i>S. mitis</i> ((b), (d), (f) and (h)) were recovered after 1 ((a) and (b)), 2 ((c) and (d)), 4 (e) and (f)) and 6 ((g) and (h)) of growth. The bacteria were Gram-stained and microscopically examined with a 1000X magnification.</p
Growth of <i>Salmonella</i> on F-Asn as sole nitrogen source.
No full text<p>Growth of wild-type MA43 and <i>fraB1</i>::kan mutant MA59 on F-Asn. Bacteria were grown overnight in LB at 37°C shaking, centrifuged, resuspended in water, and subcultured 1∶1000 into NCE medium lacking a nitrogen source (NCE-N) but containing the indicated carbon source at 5 mM. The optical density at 600 nm was then read at time points during growth at 37°C with shaking. Controls included NCE-N with no carbon source, NCE-N with 5 mM glucose, and NCE-N with glucose that was not inoculated, as a sterility control. Each point represents the mean of four cultures and error bars represent standard deviation.</p
A Self-Assembling Lanthanide Molecular Nanoparticle for Optical Imaging
No full textChromophores that incorporate f-block elements have considerable potential for use in bioimaging applications because of their advantageous photophysical properties compared to organic dye, which are currently widely used. We are developing new classes of lanthanide-based self-assembling molecular nanoparticles as reporters for imaging and as multi-functional nanoprobes or nanosensors for use with biological samples. One class of these materials, which we call lanthanide "nano-drums", are homogeneous 4d-4f clusters approximately 25 to 30 angstrom in diameter. These are capable of emitting from the visible to near-infrared wavelengths. Here, we present the synthesis, crystal structure, photophysical properties and comparative cytotoxicity data for a 32 metal Eu-Cd nano-drum [Eu8Cd24L12(OAc)(48)] (1). We also explored the imaging capabilities of this nano-drum using epifluorescence, TIRF, and two-photon microscopy platforms.Welch Foundation F-816, F-1018, F1515Ministry of High Education (MOHE), Malaysia under High Impact Research (HIR) - MOHE project UM.C/625/1/HIR/MoE/CHAN/13/6 H-50001-00-A000034NIH/NIAID 1U01AI078008-3Centre for Blast Injury Study at Imperial College LondonCPRIT R1003NIH-NCI CA68682National Institutes of HealthNational Science FoundationCancer Prevention Research Institute of TexasNational Science Foundation CHE-0741973Chemistr
Growth of <i>Salmonella</i> on F-Asn in the presence or absence of tetrathionate or oxygen.
No full text<p>Growth of wild-type MA43 and <i>fraB1</i>::kan mutant MA59 on 5 mM F-Asn or 5 mM glucose anaerobically (A and B) or aerobically (C and D) in the presence (A and C) or absence (B and D) of 40 mM tetrathionate (S<sub>4</sub>0<sub>6</sub><sup>2−</sup>). Bacteria were grown overnight in LB at 37°C shaking, centrifuged, resuspended in water, and subcultured 1∶1000 into NCE medium containing the indicated carbon source. The optical density at 600 nm was then read at time points during growth at 37°C with shaking. Each point represents the mean of four cultures with error bars indicating standard deviation.</p
A proposed model of Fra protein localization and functions.
No full text<p>A proteomic survey of subcellular fractions of <i>Salmonella</i> previously identified FraB (the deglycase) as cytoplasmic and FraE (the asparaginase) as periplasmic <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004209#ppat.1004209-Brown1" target="_blank">[79]</a>. Therefore, it is possible that F-Asn is converted to F-Asp in the periplasm by the asparaginase and that the transporter and kinase actually use F-Asp as substrate rather than F-Asn. The FraD kinase of <i>Salmonella</i> shares 30% amino acid identity with the FrlD kinase of <i>E. coli</i>. FrlD phosphorylates F-Lys to form F-Lys-6-P <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004209#ppat.1004209-Wiame2" target="_blank">[28]</a>. Therefore, we hypothesize that FraD phosphorylates F-Asp to form F-Asp-6-P. The FrlB deglycase of <i>E. coli</i> shares 28% amino acid identity with FraB of <i>Salmonella</i>. The FrlB deglycase converts F-Lys-6-P to lysine and glucose-6-P <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004209#ppat.1004209-Wiame2" target="_blank">[28]</a>, so we hypothesize that FraB of <i>Salmonella</i> converts F-Asp-6-P to aspartate and glucose-6-P.</p
Growth of wild-type and <i>fraB1</i>::kan mutant <i>Salmonella</i> on Amadori products.
No full text<p>Growth of wild-type MA43 and <i>fraB1</i>::kan mutant MA59 on F-Asn (A), F-Arg (B), F-Lys (C), asparagine, arginine, lysine, or glucose (D). Bacteria were grown overnight in LB at 37°C shaking, centrifuged, resuspended in water, and subcultured 1∶1000 into NCE medium containing the indicated carbon source at 5 mM. The optical density at 600 nm was then read at time points during growth at 37°C with shaking. Controls included NCE with no carbon source, and NCE with glucose that was not inoculated, as a sterility control (D). E) Complementation of a <i>fraB1</i>::kan mutation with plasmid pASD5006 encoding the <i>fra</i> island (ASD6000) or the vector control, pWSK29 (ASD6010). Each point in (A)–(E) represents the mean of three cultures with error bars indicating standard deviation. F) The structure of F-Asn (CAS#34393-27-6).</p
Fructose-asparagine is a primary nutrient during growth of Salmonella in the inflamed intestine.
No full textSalmonella enterica serovar Typhimurium (Salmonella) is one of the most significant food-borne pathogens affecting both humans and agriculture. We have determined that Salmonella encodes an uptake and utilization pathway specific for a novel nutrient, fructose-asparagine (F-Asn), which is essential for Salmonella fitness in the inflamed intestine (modeled using germ-free, streptomycin-treated, ex-germ-free with human microbiota, and IL10-/- mice). The locus encoding F-Asn utilization, fra, provides an advantage only if Salmonella can initiate inflammation and use tetrathionate as a terminal electron acceptor for anaerobic respiration (the fra phenotype is lost in Salmonella SPI1- SPI2- or ttrA mutants, respectively). The severe fitness defect of a Salmonella fra mutant suggests that F-Asn is the primary nutrient utilized by Salmonella in the inflamed intestine and that this system provides a valuable target for novel therapies
Impact of molecular genetic profle of meningiomas on the clinical course and recurrence using combined modality treatment
No full textIntroduction. Meningioma is one of the most common central nervous system tumors, accounting for 39.7 % of all primary brain tumors. The tumor originates from arachnoid meningothelial cells and is characterized by a wide range of histological types classified into 15 subtypes. The histological classification of meningiomas allows us to predict meningioma behavior and the risk of disease recurrence, as well as to define treatment strategies. However, clinical outcomes in histological subgroups of patients are often inconsistent with the histological grade of malignancy. Thus, a more reliable method is needed both to determine the histological subtype of the tumor and to predict the clinical course of the disease with the potential for targeted treatment.The purpose of the study was to summarize the available data on the effect of results of the genomic and proteomic tumor analysis on carcinogenesis with the relationship between the mutational changes and noninvasive diagnosis, treatment and the course of the disease.Material and Methods. Literature search was carried out in the PubMed, Elibrary system, publications were included mainly from 2010 to 2023. with the identification of articles by the keyword “genetic analysis of meningiomas” and synonyms. 550 articles were found, of which 55 were used to write a literature review.Conclusion. The study of the molecular genetic profile of meningiomas will improve the classification and establish a correlation with MRI data, the course of the disease and prognosis
Competitive index measurements of a <i>fraB1</i>::kan mutant during <i>in vitro</i> growth.
No full text<p>Cultures were grown overnight in LB, pelleted and washed in water, subcultured 1∶10,000 and grown for 24 hours at 37°C in NCE minimal medium containing 5 mM F-Asn, aerobically or anaerobically, in the presence or absence of tetrathionate (S<sub>4</sub>O<sub>6</sub><sup>2−</sup>), as indicated. A) Anaerobic growth in the presence of tetrathionate, B) anaerobic growth in the absence of tetrathionate, C) aerobic growth in the presence of tetrathionate, D) aerobic growth in the absence of tetrathionate. Each data point represents the CI from one culture with the median shown by a horizontal line. Statistical significance of each group being different than 1 was determined by using a one sample Student's <i>t</i> test. Statistical significance between select groups was determined using a Mann-Whitney test. ** = P value<0.01, *** = P value<0.001.</p
