1,721,253 research outputs found

    Metabolic aspects in bovine preovulatory and in vitro matured oocytes: a cytochemical approach

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    Studies conducted with the mouse and, to a lesser extent, the rabbit provide the vast majoruty of the existing informations on the metabolic enzymes of the mammalina ovum. In sharp contrast such data are almost non existent for domestic animal ova. In the present preliminary study we used a cytochemical quantitative assay to measure succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activities in preovulatory oocytes collected from ovaries of non pregnant cows at the local abbatoir

    Fetal adnexa derived stem cells from domestic animal : progress and perspectives

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    The fetal adnexa such as umbilical cord, amnion and amniotic fluid have been proposed as ideal sources of different stem cell lineages. Use of adnexal tissue has many potential advantages, including the noninvasive nature of the isolation procedure, the large tissue mass from which cells can be harvested with high efficiency and the potential of these cells to differentiate. Moreover, particularly in human medicine, the harvesting of these tissues is more ethically acceptable making these sources of stem cells very attractive for regenerative therapies and biotechnological applications. The adnexal tissue cells preserve some of the characteristics of the primitive embryonic layers from which they originate. Indeed, many studies indicate that these stem cells exhibit some features of embryonic stem cells as expression of embryonic markers and proliferation capability, without showing immunogenicity. However, the differentiation potential of these cells, either in vivo or in vitro, is intermediate between the pluripotent embryonic stem cells and the multipotent adult stem cells. Non-embryonic extra-fetal derived stem cells have opened new perspectives for developmental biology and for regenerative medicine, not only in humans but also in animals. In this update, we report the state of the art of fetal adnexa-derived stem cells from domestic animals and analyze their applications and potential uses in veterinary medicine

    Valutazione dell'attività telomerasica in neoplasie mammarie della cagna :un potenziale strumento diagnostico

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    Negli ultimi anni nella letteratura scientifica si è sviluppato un considerevole interesse verso lo studio dell’attività telomerasica come target per l’intervento diagnostico e terapeutico in oncologia. Un vasto numero di lavori ha, infatti, dimostrato l’espressione e l’attività dell’enzima telomerasi nella maggioranza dei tessuti tumorali umani e scarsa o non determinabile attività nei tessuti somatici normali. Studi analoghi si stanno compiendo nelle specie domestiche, in particolare in quella canina e felina. In questo lavoro è stata valutata l’attività telomerasica in campioni di tumori mammari canini prelevati sia con biopsia e, dalla comparazione con i corrispondenti campioni mammari normali provenienti dagli stessi soggetti, è stato possibile caratterizzare, sotto il profilo dell’attività telomerasica, alcuni tipi di neoplasie mammarie
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