6 research outputs found
College of Engineering Drexel E-Repository and Archive (iDEA)
No full textwww.library.drexel.edu The following item is made available as a courtesy to scholars by the author(s) and Drexel University Library and may contain materials and content, including computer code and tags, artwork, text, graphics, images, and illustrations (Material) which may be protected by copyright law. Unless otherwise noted, the Material is made available for non profit and educational purposes, such as research, teaching and private study. For these limited purposes, you may reproduce (print, download or make copies) the Material without prior permission. All copies must include any copyright notice originally included with the Material. You must seek permission from the authors or copyright owners for all uses that are not allowed by fair use and other provisions of the U.S. Copyright Law. The responsibility for making an independent legal assessment and securing any necessary permission rests with persons desiring to reproduce or use the Material. Please direct questions to [email protected] F M Sasoglu, A J Bohl and B E Layton Microbeam array for nN force measurement Design and microfabrication a high-aspect-ratio PDMS microbeam array for parallel nanonewton force measurement and protein printin
Towards a method for printing a network of chick forebrain neurons for biosensor applications
No full textPaper presented at the 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07, Lyon, France.The primary goal of this work is to establish a
robust, repeatable method for printing arrays of neurons. This
work has two endpoints. One is to use a neural array as an
experimental testbed for investigating neuronal cell growth
hypotheses. The other endpoint is to enable the next generation
of cell-based sensors. Herein we compare microcontact printing
results previously published by our group with a new method
of dip-pen printing. We present preliminary results for neurons
growing on these microprinted arrays, assessing contact
frequencies and growth characteristics
Microfabrication procedure of PDMS microbeam array using photolithography for laminin printing and piconewton force transduction on axons
No full textMicrofabrication procedure of PDMS microbeam array using photolithography for laminin printing and piconewton force transduction on axons
No full textPaper presented at 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS), New York, NY: Aug 30 - Sept 3, 2006.The purpose of this paper is to introduce our
design for transducing forces on the order of tens of
piconewtons by optically measuring deflection of a
microfabricated beam tip as it pulls on an array of
flexible structures such as axons in an array of lamininprinted
neurons. To achieve this we have designed
polymeric beams with spring constants on the order of
10pN/μm. We have fabricated circular microbeams with
Sylgard® polydimethylsiloxane (PDMS). The elastic
modulus of PDMS was determined experimentally using
a microscale and a micrometer at different
concentrations of curing agent and base agent and found
to be on the order of 100kPa. The designed geometry is a
100x100 tapered microcone array with each beam
having a length of 100μm, and a base diameter of 10 μm.
A SU-8 negative photoresist is etched using
photolithography and used as a mold for PDMS soft
lithography. PDMS was injected into the mold and the
array peeled from the mold
Design and microfabrication of a high-aspect-ratio PDMS microbeam array for parallel nanonewton force measurement and protein printing
No full textJournal of Micromechanics and Microengineering, 17(3): pp. 623-632.Cell and protein mechanics has applications ranging from cellular development
to tissue engineering. Techniques such as magnetic tweezers, optic tweezers, and atomic
force microscopy have been used to measure cell deformation forces on the order of
piconewtons to nanonewtons. In this study, an array of polymeric polydimethylsiloxane
(PDMS) microbeams with diameters of 10-40μm and lengths of 118μm was fabricated
from Sylgard® with curing agent concentrations ranging from 5% to 20%. Resulting
spring constants were 100-300nN/μm. The elastic modulus of PDMS was determined
experimentally at different curing agent concentrations and found to be 346kPa to 704kPa
in a millimeter-scale array and ~1MPa in a microbeam array. Additionally, the
microbeam array was used to print laminin for the purpose of cell adhesion. Linear and
non-linear finite element analyses are presented and compared to the closed-from
solution. Conclusion: The highly compliant, transparent, biocompatible PDMS may offer
a method for more rapid throughput in cell and protein mechanics force measurement
experiments with sensitivities necessary for highly compliant structures such as axons
