170 research outputs found
Feedback sémantique dans un environnement de recherche d'informations géographiques
No full text@inproceedings{CN-LIMAM-2003, author = {Limam, M. O. A. and Dumoncel, F. and Gaio, M.}, title = {Feedback sémantique dans un environnement de recherche d'informations géographiques}, booktitle = {15eme conférence francophone sur l'Interaction Homme-Machine (IHM'03)}, year = {2003}, address = {Caen, France}, month = {novembre} }National audienc
Dialogue Homme-Machine pour l'accès à l'information géographique, Accès intelligent aux documents multimédias sur l'Internet
No full text@inproceedings{CN-LIMAM-2002, author = {Limam, M. O. A.}, title = {Dialogue Homme-Machine pour l'accès à l'information géographique, Accès intelligent aux documents multimédias sur l'Internet}, booktitle = {Journées Francophones D'Accès Intelligent aux Documents Multimédias sur l'Internet, Media Net}, year = {2002}, address = {Sousse, Tunisie}, pages = {105-116} }National audienc
Intéraction bimodale pour l'accès à l'information
No full text@inproceedings{CN-LIMAM-2002-2, author = {Limam, M. O. A. and Gaio, M.}, title = {Intéraction bimodale pour l'accès à l'information}, booktitle = {14eme conférence Francophone sur l'Intéraction Homme-Machine (IHM'02)}, year = {2002}, address = {Poitiers, France} }National audienc
Construction and specification of spatial configurations in a geographical information retrieval context
No full text@inproceedings{CI-LIMAM-2004, author = {Limam, M. O. A. and Gaio G.}, title = {Construction and specification of spatial configurations in a geographical information retrieval context}, booktitle = {proceedings of the 12th International Conference on Geoinformatics (Geoinformatics'04)}, year = {2004}, address = {Gavle, Sweden}, month = {June} }International audienc
Génération de texte guidée par un focus : description textuelle de schémas géographiques
No full text@inproceedings{CN-LIMAM-2002-3, author = {Limam, M. O. A. and Gaio, M.}, title = {Génération de texte guidée par un focus : description textuelle de schémas géographiques}, booktitle = {5ème colloque international sur le document électronique (CIDE'02)}, year = {2002}, address = {Hammamet, Tunisie}, pages = {289-308} }National audienc
An end user dedicated new language for geographical
No full text@inproceedings{CI-LIMAM-2003, author = {Limam, M. O. A. and Gaio, M. and Madelaine J.}, title = {An end user dedicated new language for geographical}, booktitle = {International Conference on Human-Computer Interaction (HCI'03)}, year = {2003}, address = {Crete, Greece} }International audienc
A Design Environment for Audio Services
No full text@inproceedings{CI-LIMAM-2005, author = {Limam, M. O. A. and Madelaine J. and Enjalbert P.}, title = {A Design Environment for Audio Services}, booktitle = {11th International Conference on Human-Computer Interaction (HCI'05)}, year = {2005}, address = {Las Vegas, Nevada, USA}, month = {July}, publisher = {Mira Digital Publishing} }International audienc
Production of Anti-Methicillin-Resistant Staphylococcus Activity from Bacillus subtilis sp Strain B38 Newly Isolated from Soil
No full textB38 bacterial strain, isolated from Tunisian soil showed a strong antimicrobial activity. Based on biochemical characterization and 16S rDNA sequence analysis, B38 strain was identified as Bacillus subtilis. Cell culture supernatant showed antibacterial activity against clinical isolates of methicillin-resistant Staphylococcus species and several Gram-positive and Gram-negative bacteria. Antifungal activity against phytopathogenic fungi was also observed. Antibacterial activity production started at early exponential growth phase, and maximum activity was reached at the stationary phase. This antibacterial activity was neither affected by proteases, lipase, and organic solvents, nor by surfactants. It was stable over a wide pH range and still active after autoclaving at 121 degrees C during 20 min. Thin layer chromatography followed by bioautography assay allowed the detection of four active spots with R(f) values of 0.30, 0.47, 0.70, and 0.82. The single spot with R(f) 0.30 showed antifungal activity, whereas the spots with R(f) values of 0.47, 0.70, and 0.82 exhibited antibacterial activity
A new antibacterial and antioxidant S07-2 compound produced by Bacillus subtilis B38
No full textAn antibacterial compound, S07-2, was purified to homogeneity by hydrophobic
interaction, anion exchange, C18 reverse-phase and HS PEG HPLC. The molecular
mass of S07-2 was 905.6 Da as determined by MS. The S07-2 compound was
resistant to high temperatures (up to 100 1C) and could withstand a wide range of
pH from 3 to 10. In addition, its antibacterial activity was preserved after
treatment with proteases. Biochemical characterization revealed its cyclic peptide
structure. This compound showed a bactericidal effect against important food-
spoilage bacteria and food-borne pathogens including Listeria monocytogenes and
Enterococcus faecalis with lethal concentration values of 62.5 mg mL1
and against
Salmonella enteritidis at a concentration of 31.25 mg mL1
. However, no cytotoxic
effect against human erythrocytes was recorded. Furthermore, the S07-2 com-
pound displayed a remarkable Fe
21
-chelating activity (EC50 = 9.76 mg mL1
) and
1-diphenyl-2-picrylhydrazyl-scavenging capacity (IC50 = 65 mg mL1
). All these
chemical and biological features make S07-2 a useful compound in the food
industry as a natural preservative
Anti HSV-2 activity of Peganum harmala (L.) and isolation of the active compound.
No full textGenital herpes is a sexually transmitted disease caused by herpes simplex virus type 2 (HSV-2). Nucleoside analogues such as acyclovir (ACV) are the usual therapy for treating HSV infection. However, the overuse of this drug has led to the emergence of resistant strains. Therefore, the search for new alternative or complementary molecules to overcome this obstacle is needed. In this objective, Peganum harmala was investigated for its HSV-2 activity. The organic extracts of the different plant organs were evaluated for their cytotoxicity on Vero cells by the MTT test and anti HSV-2 activity by plaque reduction assay. Only the methanol seeds extract was active with a 50% inhibitory concentration (IC50) and a selectivity index (SI) of 161 and 13.2 μg/mL, respectively. In addition, the study of the antiviral mode of action revealed that this extract exerts a virucidal action both during the entry of viruses and the release of the newly formed virions, whereas no cell protection effect was observed. The active compound was isolated by bio-guided purification using thin layer chromatography (TLC) and identified by GC-MS and HPLC-DAD-ESI-MSn as harmine. The combination of harmine standard compound with ACV showed a combination index (CI) of 0.5 indicating that these two compounds have a synergic effect. This data suggests that harmine could be associated to ACV to improve the treatment of genital herpes essentially for the immunocompromised patients
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