1,721,034 research outputs found
Polyphenol-Rich Botanicals Modulate Intestinal Oxidative Stress and Epithelial Integrity in vitro
No full textEstablishment and characterization of an SV40 immortalized chicken intestinal epithelial cell line
Full text linkABSTRACT: Primary chicken intestinal epithelial cells or 3D enteroids are a powerful tool to study the different biological mechanisms that occur in the chicken intestine. Unfortunately, they are not ideal for large-scale screening or long-term studies due to their short lifespan. Moreover, they require expensive culture media, coatings, or the usage of live embryos for each isolation. The aim of this study was to establish and characterize an immortalized chicken intestinal epithelial cell line to help the study of host–pathogen interactions in poultry. This cell line was established by transducing into primary chicken enterocytes the SV40 large-T antigen through a lentiviral vector. The transduced cells grew without changes up to 40 passages maintaining, after a differentiation phase of 48 h with epidermal growth factor, the biological properties of mature enterocytes such as alkaline phosphatase activity and tight junction formation. Immortalized enterocytes were able to generate a cytokine response during an inflammatory challenge, and showed to be susceptible to Eimeria tenella sporozoites invasion and generate a proper immune response to parasitic and lipopolysaccharide (Escherichia coli) stimulation. This immortalized cell line could be a cost-effective and easy-to-maintain model for all the public health, food safety, or research and pharmaceutical laboratories that study host–pathogen interactions, foodborne pathogens, and food or feed science in vitro
A thymol-based blend of botanicals can protect intestinal cells during a chronic inflammatory challenge in vitro
No full textThyme essential oil and its phenolic derivatives are effective anticoccidial and antibacterial against Eimeria tenella and Clostridium perfringens in vitro
No full textCoccidiosis, caused by protozoan parasites of the genus Eimeria, is one of the main threats for aviculture, as it causes severe hemorrhagic diarrhea and acts as a predisposing factor for necrotic enteritis, whose causative agent is Clostridium perfringens. The aim of this study was to investigate in vitro the anticoccidial and anticlostridial efficacy of thyme essential oil and its main phenolic bioactive compounds (thymol, carvacrol and eugenol) against E. tenella and C. perfringens, respectively by invasion and minimal inhibitory concentration (MIC) assays, in comparison with conventional antibiotics.
For the invasion assay, confluent Madin-Darby bovine kidney cells were challenged with E. tenella sporozoites (5x104/cm2), without (control) or with various treatments: salinomycin (5 ppm), thyme oil (40 ppm, thymol content 50%), thymol, carvacrol or eugenol (20 ppm each). After 2 and 24 h at 37°C the efficiency of invasion was determined by counting free sporozoites left in the medium outside cells. For the MIC assay, a broth microdilution method on 96-well microtiter plates was used to test a range of concentrations (2-fold dilutions) of bacitracin (64-0.5 ppm), thyme essential oil (2000-62.5 ppm, thymol content 50%), thymol, carvacrol or eugenol (7.5-0.23 mM). Clostridium perfringens ATCC® 13124 strain (105 CFU/mL) was incubated with the substances under anaerobic conditions in Reinforced Clostridial Medium at 37°C for 24 h. After incubation, the absorbance at 630 nm was read with a spectrophotometer to measure bacterial growth. The MIC was defined as the lowest concentration that resulted in null absorbance. Data were analyzed with one-way ANOVA and differences considered significant at p<0.05.
The invasion assay showed that all the treatments significantly inhibited E. tenella invasion efficiency compared to the control already after 2 hours by 55% on average and similarly at 24 h with the highest reduction for salinomycin (-54% compared to control) and an average reduction of 35% for all the botanical treatments (p<0.001). Against C. perfringens the MIC values were found for bacitracin at 2 ppm, for thymol and carvacrol at 1.87 mM, for eugenol at 3.75 mM and for thyme oil at 500 ppm (equivalent to 1.66 mM of thymol).
In conclusion, both thyme essential oil and its main phenolic bioactive compounds demonstrated anticoccidial and bactericidal activity against E. tenella and C. perfringens in vitro. Thus, these botanicals could be valuable tools to counteract the causative agents of avian coccidiosis and necrotic enteritis
Effet d’un mélange microencapsulé de substances aromatiques chez le porcelet sevré, infecté artificiellement avec Escherichia coli F4
No full textThyme oil and thymol as zinc oxide alternatives to protect cultured enterocytes from E. coli K88/F4 during an in vitro infection
No full textAntimicrobial kinetics of a blend of bioactive compounds and its role in maintaining epithelial integrity during a Clostridium perfringens challenge on a chicken intestinal cell line
No full textP117. Development of an in vitro model to study intestinal integrity during an E. coli K88 challenge
No full textProtective effect of a microencapsulated mixture of botanicals in the feed of weaning piglets artificially challenged with Escherichia coli lipopolysaccharide
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