6 research outputs found
Production Methods of D-Allulose
No full textRare sugars are of great interest as alternative sweeteners because they are beneficial for human health and have a high industrial value. The existence of rare sugars in nature in very limited quantities has encouraged studies to convert common sugars obtained from plants into rare sugars by enzymatic, chemical or other methods. D-allulose, which has a very important place among rare sugars, is a sugar that stands out with its low calorie and sweetness very close to sucrose. It has the ability to regulate many biological functions such as lowering blood glucose level, improving insulin resistance, reducing fat accumulation in the body and reducing fever, as well as having high solubility and positive effects on food tissue, making the use of this sugar more efficient in food processing. D-allulose is known as "indigestible carbohydrate". It occurs naturally in many fruits and beverages and some cereal products. Today, D-allulose can be produced in many ways such as plant extraction, chemical synthesis, enzymatic conversion and can be safely used in the production of some foodstuffs. In this review, D-allulose production methods are presented, differences in these methods and their advantages and disadvantages are compared to each other. © 2022 by the Author(s)
Fatty acids, triglycerides, tocol, and sterol contents of oils of some Moringa seed varieties
No full textThree moringa oliefera varieties (MOMAX 3, ODC and PKM-1) seeds were used in this study. The oil was extracted from the seeds of ODC, PKM1 and MOMAX 3 moringa varieties by cold press (CP) and the solvent extraction (SE) method. Fatty acids, triglycerides, tocols (tocopherols and tocotrienols) and sterol contents of moringa seed oils obtained by two different methods were determined and compared with one another. The crude oil yield was between 26.46 - 28.19% in solvent extraction and 24.20 - 26.30% in the cold press method. It was determined that the main fatty acid in moringa seed oils (ODC, PKM1 and MOMAX 3) was Oleic acid (63.31 - 70.04%). Other dominant fatty acids were determined to be palmitic acid (16:0) (5.59-7.26%), stearic acid (18:0) (5.37-5.89%), oleic acid (18:1 n9) (%63.31-70.04), arachidic acid (20:0) (2.67 - 3.71 %) and behenic acid (22:0) (3.82 5.73%). It was determined that the main triglyceride in moringa seed oils was triolein (000; 35.63 - 36.50%), the main sterol was [3-Sitosterol (39.04 - 42.11 %) and the main tocopherol was a-tocopherol (15.88 -18.91 pg/g).Adana Al- parslan T0rke Science and Technology University Scientific Research Projects Unit [20332002]Acknowledgment This study was financially supported by Adana Al- parslan T0rke Science and Technology University Scientific Research Projects Unit (Project number: 20332002)
Fatty acids, sterols and triglycerides composition of cold pressed oil from pomegranate seeds
No full textPomegranate seed oil (PSO) was obtained by cold pressing of seeds of pomegranate (Punica granatum L.) grown in Turkey. Total crude oil was obtained from pomegranate seeds on a 14.62% dry matter (DM) and it was determined to having a high content of polyunsaturated fatty acids (87.82%). The dominant fatty acid in pomegranate seeds was determined to be punicic acid (C18:3n-5; 9c, 11t, 13c) and it was found that it constitutes about 76.14% of the total fat content. It was observed that the total tocopherol content of PSO was 258.40 mg/100g and the main tocopherol was alpha-tocotrienol and the others were alpha-tocopherol (189.89 mg/100g) and gamma-tocopherol (53.14 mg/100g), respectively. The total sterol content of PSO was 930 mg/100g with a predominance of beta-sitosterol in our study and the beta-sterol content was 87.32%. The major triacylglycerols (TAG) in pomegranate seed oil were determined to be Stearic-Punicic-Punicic acid (SPuPu; 4.34%), Oleic-Punicic-Linoleic acid (OPuL; 1.10%) and Palmitic-Punicic-Linoleic acid (PPuL; 0.71%), respectively
Recombinant D-tagatose 3-epimerase production and converting fructose into allulose
No full textIn this study, D-tagatose 3-epimerase gene was amplified from Escherichia coli JM109 by PCR and re-cloned into E. coli JM109 using HincII digested pUC18 cloning vector. Digestion of recombinant plasmid by HincII and PCR amplification of the gene from recombinant plasmid produced 789 bp gene band on agarose gel which indicated the gene integration. The host cell was grown in a shaking incubator at 37 degrees C for 24 hr and then the optical density (OD) of the cells was measured in a spectrophotometer at 600 nm wavelengths. When the OD600 value reached 0.61, the protein expression was induced by the addition of 0.1 mM IPTG into the growth medium. Through His-select gel column purification, highly purified and stable DTE protein was produced. The conversion rate of D-fructose into D-allulose was determined by HPLC. The native and recombinant enzymes could effectively convert D-fructose into D-allulose with a turnover ratio of 20.76%. Novelty impact statement A recombinant enzyme, D-tagatose 3-epimerase, was produced and D-fructose was converted into D-allulose using this recombinant enzyme.REPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND FORESTRY GENERAL DIRECTORATE OF AGRICULTURAL RESEARCH AND POLICIES [TAGEM-18/AR-GE/30]REPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND FORESTRY GENERAL DIRECTORATE OF AGRICULTURAL RESEARCH AND POLICIES, Grant/Award Number: TAGEM-18/AR-GE/3
Some Nutraceutical and Bioactive Compounds of Seed Oils Obtainedfrom Seeds of Different Gundelia Varieties
No full text<p>The goal of this research was aimed to investigate the nutraceutical and bioactive compounds of Gundelia seed and its seed oils. The contents of fatty acids, triglycerides, sterols, tocopherol, tocotrienol (tocols) and some physicochemical properties of the cold pressed Gundelia seed oils were analyzed by HPLC and GC devices. The main fatty acid was found to be linoleic acid (45.078-75.113%), which is a polyunsaturated fatty acid (PUFA), and the main triglyceride structure was LLL (trilinolein, 13.39-44.6%). The main sterol was beta-sterol (37.65-72.62%) and the main tocol was alpha-tocopherol (31.10-77.95 mu g/g). It was determined that the total phenolic content of the oils of Gundelia seeds varied between 974.55 and 3439.09 mu g GAE/mg oil and their antioxidant capacity ranged between 113.56 and 287.26 mg trolox/l. The oils obtained from the seeds of different Gundelia have similar properties to some edible oils and have a rich content in terms of nutraceutical and bioactive compounds.</p>
Vâsînî el-Aʿrac'ın 'Hârisetu'z-Zilâl' adlı romanında yapı ve tema
No full textVÂSÎNÎ EL-AʿRAC’IN ‘HÂRİSETU’Z-ZİLÂL’ ADLI ROMANINDA YAPI VE TEMA İhsan DEMİRTAŞ Erciyes Üniversitesi, Sosyal Bilimler Enstitüsü Yüksek Lisans Tezi, Temmuz 2024 Danışman: Prof. Dr. Metin PARILDI ÖZET Bu çalışmada Cezayir romanının önde gelen isimlerinden biri olan Vâsînî el-Aʿrac’ın yazdığı ve Fransa'da yayımlanan en iyi romanlar arasında seçilen ‘Hârisetu'z-Zilâl’ adlı roman incelendi. Hârisetu'z-Zilâl, Cezayir'deki bağımsızlık savaşı sonrası otuz yıllık süreçte ve 90'lı yıllarda patlak veren iç savaş döneminde Cezayir halkının yaşadığı sıkıntıları anlatmaktadır. Cezayir halkının maruz kaldığı baskı ve zulmün yanı sıra şehirdeki tarihi ve kültürel yapının nasıl tahrip edildiğini de ele almaktadır. Mekân kurgusu, Cervantes'in Cezayir esaretine dayanmaktadır. Romanın genel atmosferi karamsardır, ancak “Hârisetu'z-Zilâl efsanesi” gibi umudu simgeleyen unsurlar da bulunmaktadır. Çalışmanın başında, genel olarak çağdaş Arap edebiyatında ve özel olarak Cezayir edebiyatında roman türünün gelişmesi üzerinde kısaca duruldu, daha sonra Vâsînî el-Aʿrac’ın hayatı ve eserleri hakkında bilgi verildi. Romanın incelenmesine ayrılan bölümlerde Hârisetu’z-Zilâl’da yapısal ögeler, dil ve anlatım özellikleri üzerinde duruldu. Romanda tercih edilen karakterler, mekânlar ve bunlara ait özellikler detaylı bir şekilde incelendi. Çalışmada, yazarın vermek istediği mesajı ve olayların yaşandığı dönemin özelliklerini anlama ve aktarmaya öncelik verildi. Romandaki olaylar, şahsiyetler ve mekânlara, Cezayir tarihindeki olaylar, şahsiyetler ve mekânlar göz önünde bulundurularak bütüncül bir bakış açısıyla yaklaşıldı, romanın gerçek hayatla olan ilgisi tespit edilerek analiz edildi. Anahtar Kelimeler: Arap Dili ve Belagati, Modern Arap Edebiyatı, Cezayir Romanı, Vasini el-Aʿrac, Harisetu’z-Zilal STRUCTURE AND THEME IN WASINI AL-A'RAJ'S NOVEL ‘HARISAT AL-ZILAL’ İhsan DEMİRTAŞ Erciyes University, Graduate School of Social Sciences Master's Thesis, July 2024 Supervisor: Prof. Metin PARILDI ABSTRACT In this study, the novel Hārisat al-Zilāl', written by one of the leading figures of the Algerian novel, Wāsīnī al-Aʿraj, and selected among the best novels published in France, is analyzed. Hārisat al-Zilāl describes the hardships of the Algerian people in the thirty years after the Algerian war of independence and during the civil war that broke out in the 90s. In addition to the oppression and persecution that the Algerian people were subjected to, it also deals with how the historical and cultural structure of the city was destroyed. The setting is based on Cervantes' Algerian captivity. The general atmosphere of the novel is pessimistic, but there are also elements that symbolize hope, such as the "legend of Hārisat al-Zilāl ". At the beginning of the study, the development of the novel genre in contemporary Arabic literature in general and Algerian literature in particular was briefly discussed, followed by information on the life and works of al-Wāsīnī al-Aʿraj. In the chapters devoted to the analysis of the novel, the structural elements, language and narrative features of Hārisat al-Zilāl were emphasized. The characters, places and their characteristics were analyzed in detail. In the study, priority was given to understanding and conveying the message the author wanted to give and the characteristics of the period in which the events took place. The events, personalities and places in the novel were approached from a holistic perspective, taking into account the events, personalities and places in Algerian history, and the relevance of the novel to real life was determined and analyzed. Keywords: Arabic Language and Rhetoric, Modern Arabic Literature, Algerian Novel, Wasini al-A'raj, Shadows Guardian </p
