17 research outputs found

    Organic Edible Insects—What Would It Take?

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    Edible insect farming represents a promising sector focused on producing sustainable and nutritional food and feed. Compared to conventionally farmed animals, insects are more sustainable, thanks to lower greenhouse gas emissions, efficient bioconversion, and minimal space requirements. These characteristics make edible insect farming remarkably eco-friendly: this concept aligns with the core principles of the organic supply chain. However, current organic regulations do not yet include insects. This review investigates various aspects of edible insect rearing to better understand how an “organic edible insect farm” could be established. Nine insect species that have been authorized as ingredients for the production of either food or feed were included, such as Hermetia illucens, Musca domestica, Tenebrio molitor, Alphitobius diaperinus, Locusta migratoria, Gryllodes sigillatus, Gryllus assimilis, Acheta domesticus, and Bombyx mori. Among the evaluated features of insect farming, insect welfare and the use of chemical substances (such as veterinary drugs and pesticides) are thoroughly examined in the literature review. These represent the most significant challenges given the scarce knowledge both on the well-being of insects, currently identified as non-sentient beings, and on the degradation and metabolism of drugs or pesticides that could harm the animals but also undermine consumer safety

    Toxoplasma gondii in sheep: Serological occurrence at slaughterhouse level in Italy and environmental risk factors

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    : Toxoplasmosis is a parasitic disease affecting a wide range of species, including humans, and can be responsible for important clinical manifestations such as abortion and neurological signs. Sheep show a remarkable susceptibility to its causative agent, Toxoplasma gondii, and zoonotic transmission may occur in case of consumption of undercooked meat obtained from infected animals. Toxoplasma gondii seroprevalence in sheep can significantly vary on a geographical basis, as shown by numerous surveys conducted worldwide. To investigate environmental and climate conditions that may affect the likelihood of ovine infection, 405 serum samples from selected sheep raised in 91 farms were collected from two abattoirs, with each abattoir receiving animals from two regions (1/Tuscany-Latium and 2/Campania-Basilicata). The seroprevalence of infection in all examined animals was 53.8%. Young animals (n = 165) had a lower likelihood of being T. gondii positive compared to the adults (OR = 0.21), and the seropositive rate of animals slaughtered in abattoir 2 was significantly higher than that of animals slaughtered in abattoir 1 (60.5 vs. 43.2%, p < 0.01). The significant bioclimatic variables (p < 0.05) associated with the presence of T. gondii antibodies were related to areas with a lower range of temperature and higher precipitation. In conclusion, this study expands on the interpretation of serological data, with the inclusion of environmental and climatic variables, as possible risk factors in the spread of toxoplasmosis in the study area. These findings provide novel insights to support public health measures, such as risk-based control plan, and contribute to a "One Health" approach, taking into account the environmental and climatic perspectives

    Exploring the potential of black soldier fly live larvae as a sustainable protein source for laying hens: A comprehensive study on egg quality

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    Live insect larvae were recently proposed for use in laying hens in intensive chicken farming as an innovative form of environmental enrichment. This study aimed to evaluate the effect of laying hen age and feeding with live Black Soldier Fly larvae (BSFL) on egg quality attributes, i.e., chemical composition, fatty acid (FA) profile, and metabolic profile using nuclear magnetic resonance (NMR) spectroscopy. To this aim, 108 Lohman Brown hens were housed in 27 cages (9 replicates per treatment, 4 birds per pen) and monitored between 16 and 34 weeks of age. The hens were split into three experimental groups: a control group fed a commercial diet, and two experimental groups fed the same commercial diet plus 15% or 30% of live BSFL, as fed basis on the expected daily feed intake (DFI). The experimental treatments did not affect the egg and eggshell quality attributes. The supplementation with live BSFL did not influence the chemical composition in terms of macronutrients or the main NMR profiles of egg yolk and albumen. The FA profile of the egg yolk significantly changed as the eggs from hens fed BSFL presented higher rates of SFA and PUFA (P&lt;0.05), lower rate of MUFA (P&lt;0.001), and higher rates of C18:2n 6 (P&lt;0.05) and C18:3 n3 compared to the control eggs (P&lt;0.001). There were no significant differences in the ratio of n-6 to n-3 PUFA. The age of the hens strongly affected egg quality traits (P&lt;0.001), mainly the egg weight, shell weight, shell thickness, eggshell-breaking strength, and eggshell redness (a*) and yellowness (b*), besides the metabolic profile of both egg yolk and albumen. Considering the interaction diet * age of hens, only a few significant effects occurred on egg quality attributes and FA profile. In conclusion, a supplementation with live BSFL up to 30% of DFI may be safely used in laying hen feeding without impairing egg quality

    An Investigation of Virulence Genes of Staphylococcus aureus in Autologous Vaccines Against Sheep Mastitis

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    Staphylococcus aureus is well known to be the primary causal agent of clinical or subclinical mastitis in dairy sheep. The production of virulence factors allows S. aureus strains to cause mastitis. In the present study, 96 strains isolated from dairy sheep farms used for the production of autologous vaccines were tested for enterotoxin and hemolysin genes by PCR. In addition, 14 strains isolated from half udders of ewes with subclinical mastitis belonging to a single farm were also tested for the same genes. The phylogenetic trees were constructed, and spatial analysis was performed. Overall, 20 gene patterns were identified, but 43.64% of the tested strains showed the same profile (sec+, sel+, hla+, hld+, hlgAC+). Considering only the enterotoxin genes, four profiles were identified while the evaluation of the hemolysin genes revealed the presence of 12 gene patterns. In the farm with subclinical mastitis, six gene profiles were found. Spatial analysis of the isolated strains and their virulence genes did not show a specific pattern. The present study highlights the importance of identifying and analyzing virulence genes of S. aureus strains involved in dairy sheep mastitis, and the presence of different strains in the same farm

    Effect of PrP genotype and route of inoculation on the ability of discriminatory Western blot to distinguish scrapie from sheep bovine spongiform encephalopathy

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    Procedures for discriminating scrapie from bovine spongiform encephalopathy (BSE) in sheep are relevant to ascertain whether BSE has entered the sheep population. This study was aimed at investigating whether the suitability of an official EU discriminative method is affected by the sheep PrP genotype and route of infection.</jats:p
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