1,721,136 research outputs found
ELECTRONIC PARAMAGNETIC RESONANCE AND OPTICAL SPECTROSCOPIC STUDY OF THE HEME-HUMAN SERUM ALBUMIN COMPLEX.
No full textPHENYL BORONIC ACID AS LIGAND FOR A BIOSPECIFIC CHROMATOGRAPHY OF ß-N-ACETYLHEXOSAMINIDASE ISOENZYMES.
No full textRicerca, didattica, competenze, reti di relazioni scientifiche e tecnologiche nel settore delle biotecnologie
No full textPregnancy modulates the expression of B-N-acetyl-hexosaminidase in rat serum and tissues
No full textSOLUBILITY OF HEMATOPORPHYRIN AND PHOTODYNAMIC DAMAGES IN LIPOSOMAL SYSTEMS: OPTICAL AND ELECTRON SPIN RESONANCE STUDIES.
No full textA distinct beta-hexosaminidase isoenzyme separated from human leukemic lymphocytes and myelocytes
No full textThe beta-hexosaminidase (EC 3.2.1.30) isoenzymes were separated on the basis of their carbohydrate moieties by an affinity chromatography using immobilized phenylboronate. Normal lymphocytes and granulocytes contain two major forms of beta-hexosaminidase, acute lymphoblastic, acute myeloblastic, chronic lymphocytic and chronic myelocytic leukemic cells contain an extra, distinct isoenzyme of beta-hexosaminidase. This extra isoenzyme may be a marker for the leukemic conversion of hematopoietic tissue
Expression of a particular beta-N-acetylglucosaminidase isoenzyme in human haematopoietic leukemic cell-lines.
No full textN-acetyl-beta-D-glucosaminidase (NAG) activity and isoenzyme profiles were studied in myeloid, histiocytic, B-lymphoid, T-lymphoid and lymphoblastoid continuous cell lines in order to determine if N-acetyl-beta-D-glucosaminidase isoenzyme expression may help to distinguish among various types of leukemic proliferation. Total NAG activity in myeloid, histiocytic, erythroleukemic cell lines were higher than Burkitt's lymphoma derived cell lines (B-lymphoid), T- or lymphoblastoid cell lines. On chromatofocusing by PBE 94 coupled with an automated enzyme assay an intermediate (I) beta-N-acetyl-glucosaminidase form, eluting between forms B and A, was found in all leukemic and in Epstein-Barr virus infected lymphoblastoid cell lines analysed. The different profiles recorded, the expression of the I form and the different I/B ratios may be useful as markers of tumour proliferation
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