162,803 research outputs found

    Too good to be true: pitfalls of usingmean Ellenberg indicator values in vegetation analyses

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    Question: Mean Ellenberg indicator values (EIVs) inherit information about compositional similarity, because during their calculation species abundances (or presence–absences) are used as weights. Can this similarity issue actually be demonstrated, does it bias results of vegetation analyses correlating mean EIVs with other aspects of species composition and how often are biased studies published? Methods: In order to separate information on compositional similarity possibly present in mean EIVs, a new variable was introduced, calculated as a weighted average of randomized species EIVs. The performance of these mean randomized EIVs was compared with that of the mean real EIVs on the one hand and random values (randomized mean EIVs) on the other. To demonstrate the similarity issue, differences between samples were correlated with dissimilarity matrices based on various indices. Next, the three mean EIV variables were tested in canonical correspondence analysis (CCA), detrended correspondence analysis (DCA), analysis of variance (ANOVA) between vegetation clusters, and in regression on species richness. Subsequently, a modified permutation test of significance was proposed, taking the similarity issue into account. In addition, an inventory was made of studies published in the Journal of Vegetation Science and Applied Vegetation Science between 2000 and 2010 likely reporting biased results due to the similarity issue. Results: Using mean randomized EIVs, it is shown that compositional similarity is inherited into mean EIVs and most resembles the inter-sample distances in correspondence analysis, which itself is based on iterative weighted averaging. The use of mean EIVs produced biased results in all four analysis types examined: unrealistic (too high) explained variances in CCA, too many significant correlations with ordination axes in DCA, too many significant differences between cluster analysis groups and too high coefficients of determination in regressions on species richness. Modified permutation tests provided ecologically better interpretable results. From 95 studies using Ellenberg indicator values, 36 reported potentially biased results. Conclusions: No statistical inferences should bemade in analyses relatingmean EIVs with other variables derived from the species composition as this can produce highly biased results, leading to misinterpretation. Alternatively, a modified permutation test using mean randomized EIVs can sometimes be used

    Aphorismes mathématiques de J. Ellenberg

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    J. S. Ellenberg est un des meilleurs mathématiciens de notre temps. Il a écrit un livre remarquable intitulé en anglais "How not to be wrong", traduit en français sous le titre "L'art de ne pas dire n'importe quoi". D'après l'auteur, " il voulait expliquer aux gens, en détail, combien les maths sont fantastiques". Nous reprenons dans cette note quelques extraits qui nous semblent contenir des idées profondes et pertinentes à propos des mathématiques, de leur enseignement et de leur heuristique (c'est-à-dire la façon de les trouver); nous les appelons des "aphorismes mathématiques"

    Nuclear Actin: A Lack of Export Allows Formation of Filaments

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    Actin has been found in nuclei of many cell types, but little is known about its form and function. A recent study has shown that a lack of specific export allows actin to accumulate in the nucleus, where it forms a network of actin filaments that may be required to stabilize the giant nucleus of the Xenopus oocyte

    Ellenberg-type indicator values for European vascular plant species

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    Aims: Ellenberg-type indicator values are expert-based rankings of plant species according to their ecological optima on main environmental gradients. Here we extend the indicator-value system proposed by Heinz Ellenberg and co-authors for Central Europe by incorporating other systems of Ellenberg-type indicator values (i.e., those using scales compatible with Ellenberg values) developed for other European regions. Our aim is to create a harmonized data set of Ellenberg-type indicator values applicable at the European scale. Methods: We collected European data sets of indicator values for vascular plants and selected 13 data sets that used the nine-, ten- or twelve-degree scales defined by Ellenberg for light, temperature, moisture, reaction, nutrients and salinity. We compared these values with the original Ellenberg values and used those that showed consistent trends in regression slope and coefficient of determination. We calculated the average value for each combination of species and indicator values from these data sets. Based on species’ co-occurrences in European vegetation plots, we also calculated new values for species that were not assigned an indicator value. Results: We provide a new data set of Ellenberg-type indicator values for 8908 European vascular plant species (8168 for light, 7400 for temperature, 8030 for moisture, 7282 for reaction, 7193 for nutrients, and 7507 for salinity), of which 398 species have been newly assigned to at least one indicator value. Conclusions: The newly introduced indicator values are compatible with the original Ellenberg values. They can be used for large-scale studies of the European flora and vegetation or for gap-filling in regional data sets. The European indicator values and the original and taxonomically harmonized regional data sets of Ellenberg-type indicator values are available in the Supporting Information and the Zenodo repository

    [Report to Chief J. E. Curry, by an unknown author #1]

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    Report to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney

    [Report to Chief J. E. Curry, by an unknown author #2]

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    Report to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney

    Self-Organization of MTOCs Replaces Centrosome Function during Acentrosomal Spindle Assembly in Live Mouse Oocytes

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    SummaryChromosome segregation in mammalian oocytes is driven by a microtubule spindle lacking centrosomes. Here, we analyze centrosome-independent spindle assembly by quantitative high-resolution confocal imaging in live maturing mouse oocytes. We show that spindle assembly proceeds by the self-organization of over 80 microtubule organizing centers (MTOCs) that form de novo from a cytoplasmic microtubule network in prophase and that functionally replace centrosomes. Initially distributed throughout the ooplasm, MTOCs congress at the center of the oocyte, where they contribute to a massive, Ran-dependent increase of the number of microtubules after nuclear envelope breakdown and to the individualization of clustered chromosomes. Through progressive MTOC clustering and activation of kinesin-5, the multipolar MTOC aggregate self-organizes into a bipolar intermediate, which then elongates and thereby establishes chromosome biorientation. Finally, a stable barrel-shaped acentrosomal metaphase spindle with oscillating chromosomes and astral-like microtubules forms that surprisingly exhibits key properties of a centrosomal spindle

    A New Model for Asymmetric Spindle Positioning in Mouse Oocytes

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    SummaryAn oocyte matures into an egg by extruding half of the chromosomes in a small polar body. This extremely asymmetric division enables the oocyte to retain sufficient storage material for the development of the embryo after fertilization. To divide asymmetrically, mammalian oocytes relocate the spindle from their center to the cortex. In all mammalian species analyzed so far, including human [1], mouse [2], cow [3], pig [4], and hamster [5], spindle relocation depends on filamentous actin (F-actin). However, even though spindle relocation is essential for fertility [6], the involved F-actin structures and the mechanism by which they relocate the spindle are unknown. Here we show in live mouse oocytes that spindle relocation requires a continuously reorganizing cytoplasmic actin network nucleated by Formin-2 (Fmn2). We found that the spindle poles were enriched in activated myosin and pulled on this network. Inhibition of myosin activation by myosin light chain kinase (MLCK) stopped pulling and spindle relocation, indicating that myosin pulling creates the force that drives spindle movement. Based on these results, we propose the first mechanistic model for asymmetric spindle positioning in mammalian oocytes and validate five of its key predictions experimentally

    Müller-Dombois, D. & Ellenberg, H. — Aims and Methods of Vegetation Ecology. New York, London, Sydney & Toronto, 1974, John Willey & Sons

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    Guillaumet J.-L. Müller-Dombois, D. & Ellenberg, H. — Aims and Methods of Vegetation Ecology. New York, London, Sydney & Toronto, 1974, John Willey & Sons. In: La Terre et La Vie, Revue d'Histoire naturelle, tome 33, n°4, 1979. pp. 658-659

    Murder on the mountain: author talk with Peter J. Wosh

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    Author talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
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