962 research outputs found

    Oral History Program Interview: Viola Elizabeth Shackelford Holliday and Grover Clevel and Holliday

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    CALIFORNIA STATE COLLEGE, BAKERSFIELD CALIFORNIA ODYSSEY The 1930s Migration to the Southern San Joaquin Valley Oral History Program Interview Between INTERVIEWEE : Viola Elizabeth Shackelford Holliday and Grover Clevel and Holliday PLACE OF BIRTH: Board Camp , Polk Countyt.Arkansas Mt . Ida, Montgomery County, Arkansa

    TEXTA Book Club in the QUT Art Museum

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    TEXTA runs as a type of book club that we have previously labelled as ‘bespoke’ (Ellison, Holliday and Van Luyn 2012). We visualise TEXTA as a meeting place between the community and the university, as a space for discussion and engagement with both visual art forms and written texts. In today’s presentation, we shall briefly establish the ‘bespoke’ bookclub. We then want to introduce the idea of TEXTA as an example of a book club that negotiates Edward Soja’s Thirdspace (1996) – a space that incorporates and extends concepts of First and Secondspace (or perceived and conceived spaces). In doing so, we showcase two recent sessions of TEXTA as case studies. We will then illustrate some ideas we have for expanding TEXTA beyond the boundaries of Brisbane city, and invite feedback on how to further extend the opportunities for community engagement that TEXTA can offer in regional areas

    Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus

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    RecG is a DNA translocase encoded by most species of bacteria. The Escherichia coli protein targets branched DNA substrates and drives the unwinding and rewinding of DNA strands. Its ability to remodel replication forks and to genetically interact with PriA protein have led to the idea that it plays an important role in securing faithful genome duplication. Here we report that RecG co-localises with sites of DNA replication and identify conserved arginine and tryptophan residues near its C-terminus that are needed for this localisation. We establish that the extreme C-terminus, which is not resolved in the crystal structure, is vital for DNA unwinding but not for DNA binding. Substituting an alanine for a highly conserved tyrosine near the very end results in a substantial reduction in the ability to unwind replication fork and Holliday junction structures but has no effect on substrate affinity. Deleting or substituting the terminal alanine causes an even greater reduction in unwinding activity, which is somewhat surprising as this residue is not uniformly present in closely related RecG proteins. More significantly, the extreme C-terminal mutations have little effect on localisation. Mutations that do prevent localisation result in only a slight reduction in the capacity for DNA repair. © 2014 The Author(s)

    Chronicles of Oklahoma

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    Article provides historical context for the songs and poetry created by cowboys making a living herding cattle in the Cherokee Outlet in the late 1800s. Shawn Holliday provides a detailed analysis of individual songs and poems included in the article

    Appendix_3rd_1 – Supplemental material for The Role of Maternal Intimate Partner Violence Victimization on Neonatal Mortality in Ethiopia

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    Supplemental material, Appendix_3rd_1 for The Role of Maternal Intimate Partner Violence Victimization on Neonatal Mortality in Ethiopia by Tenaw Yimer Tiruye, Catherine Chojenta, Melissa L. Harris, Elizabeth Holliday and Deborah Loxton in Journal of Interpersonal Violence</p

    Local artist and photographer C.W. Holliday sitting in front of a painting

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    Holliday was also the author of the book 'Valley of Youth'

    How Do Cities Grow?

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    How do cities grow? Why do we build them the way we do? Who decides what buildings get built - where they are and what they look like? What does the architecture of our cities tell us about our own cultural histories? As an architecture historian, Kathryn Holliday asks and answers these kinds of questions. She is the author most recently of the book Ralph Walker: Architect of the Century (Rizzoli, 2012) and serves as Director of the David Dillon Center for Texas Architecture, an initiative of the School of Architecture to research and promote public dialogue about architecture and urbanism in North Texas. This lecture is part of the GIS Day annual event, so Dr. Holliday will discuss a current project focused on the history of Dallas and Fort Worth as stored in maps and images. For the past three years, Dr. Holliday and the students in her course, The Life of Cities: Modernism in Context, have taken scans of maps from UT Arlington Library\u27s Special Collections and georeferenced them using ArcGIS software. By overlaying these georeferenced maps atop each other, students and other researchers can piece together a broader architectural history of the region. Once the maps were georeferenced, Dr. Holliday\u27s students compiled metadata and converted the maps into Google Earth format. During fall, 2012, UCLA\u27s Hypercities Project, a leader in digital humanities resources, began to spotlight these DFW maps

    Replication fork collisions cause pathological chromosomal amplification in cells lacking RecG DNA translocase

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    This is an open access article, shared under a Creative Commons licence. Copyright © 2009 The Authors.Duplication and transmission of chromosomes require precise control of chromosome replication and segregation. Here we present evidence that RecG is a major factor influencing these processes in bacteria. We show that the extensive DnaA-independent stable DNA replication observed without RecG can lead to replication of any area of the chromosome. This replication is further elevated following irradiation with UV light and appears to be perpetuated by secondary events that continue long after the elimination of UV lesions. The resulting pathological cascade is associated with an increased number of replication forks traversing the chromosome, sometimes with extensive regional amplification of the chromosome, and with the accumulation of highly branched DNA intermediates containing few Holliday junctions. We propose that the cascade is triggered by replication fork collisions that generate 3′ single-strand DNA flaps, providing sites for PriA to initiate re-replication of the DNA and thus to generate linear duplexes that provoke recombination, allowing priming of even further replication. Our results shed light on why termination of replication in bacteria is normally limited to a single encounter of two forks and carefully orchestrated within a restricted area, and explain how a system of multiple forks and random termination can operate in eukaryotes.The Medical Research Council and the Leverhulme Trust

    Mean transport and variability in the subpolar North Atlantic

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    The Atlantic Meridional Overturning Circulation (AMOC) plays an important role in the Earth’s heat and carbon budgets and sets the properties of the global water masses. Deep water formed in high latitudes, travel southwards as part of the AMOC’s lower limb and have properties that can be traced globally. As the AMOC’s upper limb transports warm water northwards it releases heat to the atmosphere, contributing to the moderate climate of Western Europe and impacts all aspects of high latitudinal climate. In the northern North Atlantic there is a lack of long-term transoceanic measurements, enabling estimates of the AMOC’s transport variability. For this reason, it is essential that quantification of the AMOC’s mean transports in the subpolar region are as accurate as possible. Furthermore, the possibility of quantifying the AMOC’s variability in this region could improve our understanding of the AMOC’s driving mechanisms.The Extended Ellett Line (EEL) is a repeat hydrographic section located between Iceland and Scotland and is the focus area for this study. This section captures 90% of the water flowing northwards into the Nordic Seas and half the returning dense water; two key parts of the AMOC. Data has been collected near-annually at the EEL since 1996, providing two decades of hydrographic and direct velocity measurements. The first objective of this study is to quantify the long-term averages of the currents that shape the AMOC across the EEL, using direct velocity measurements. Improved estimates of the long-term (1997-2015) mean absolute velocity field and volume, temperature and freshwater transports are presented. Analysis of the time-mean velocity field contributes to knowledge of the location and variability of the currents transporting water northwards. There is evidence that a branch of the North Atlantic Current is located at the Rockall-Hatton Plateau (RHP). The net transport at the EEL is 3.5 ± 0.9 Sv, of which there is 5.9 ± 1.4 Sv in the upper ocean and -3.3 ± 0.7 Sv in the overflow water. There is a temperature transport of 0.22 ± 0.04 PW, dominated by the transport strength, and a freshwater transport of 44.1 ± 8.8 mSv southwards. The slope current and RHP are identified as key routes for the transport of heat northwards.The second objective of this study is to assess the spatial resolution of the EEL over the slope current North of Scotland. Comparisons of the EEL spatial resolution with altimetry and the OSNAP array, shows that the slope current is better resolved in the former. The altimeters do represent the large-scale circulation pattern at the EEL, but they do not capture the narrow slope current. The OSNAP array does not have a high enough spatial resolution to resolve the key heat pathway of the slope current. This study shows the necessity of a higher sampling spatial resolution in this location
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