70 research outputs found
NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover
Get PDFBackground Most of the cells of the mammalian retina are terminally differentiated, and do not regenerate once fully developed. This implies that these cells have strict controls over their metabolic processes, including protein turnover. We report the use of metabolic labelling procedures and secondary ion mass spectrometry imaging to examine nitrogen turnover in retinal cells, with a focus on the outer nuclear layer, inner nuclear layer, and outer plexiform layer. Results We find that turnover can be observed in all cells imaged using NanoSIMS. However, the rate of turnover is not constant, but varies between different cellular types and cell regions. In the inner and outer nuclear layers, turnover rate is higher in the cytosol than in the nucleus of each cell. Turnover rates are also higher in the outer plexiform layer. An examination of retinal cells from mice that were isotopically labeled very early in embryonic development shows that proteins produced during this period can be found in all cells and cell regions up to 2 months after birth, even in regions of high turnover. Conclusions Our results indicate that turnover in retinal cells is a highly regulated process, with strict metabolic controls. We also observe that turnover is several-fold higher in the synaptic layer than in cell layers. Nevertheless, embryonic proteins can still be found in this layer 2 months after birth, suggesting that stable structures persist within the synapses, which remain to be determined
Isospectral, non-isometric Riemannian manifolds
Get PDFsummary:The author gives a survey of the history of isospectral manifolds that are non-isometric discussing the work of Milnor, Vign\'eras, Sunada, and de Turck and Gordon. She describes the construction of continuous isospectral deformations as introduced by Gordon, Wilson, De Turck et al. She also discusses the construction of isospectral plane domains due to Gordon, Webb, and Wolpert. Some new examples of isospectral non-isometric manifolds are given
Valdis Auzins and son Ollie and modified 1929 Chevrolet truck
No full textBlack & white photo of Valdis Auzins and his son Ollie in their modified 1929 Chevrolet turck in front of their homestead home at Pigeon Lake, Alberta15.0 Family Photos, 15.1.3 Family life in Alberta, 5.0 Early Life of Latvians in Albert
Correlation between family history of Hepatitis B and positive HBV serologic tests in patients attending dental clinics In Riyadh
No full textThe aim of this study Was Investigate the importance of family history In the Identification of HBV sero positive subject. A total of 121 patients attending dental clinics were recruited for this study. Inquiry about family history of hepatitis, if any. Was obtained from subject. Subjects 'who reported family history of hepatitis. or hepatitis-like conditions 'were called and sent for laboratory Investigation for hepatitis marker using the ELISA techniqu
INTRAORAL MAXILAY MOLAR DISTALIZATION
No full textThe aim of this study Was Investigate the importance of family history In the Identification of HBV sero positive subject. A total of 121 patients attending dental clinics were recruited for this study. Inquiry about family history of hepatitis, if any. Was obtained from subject. Subjects 'who reported family history of hepatitis. or hepatitis-like conditions 'were called and sent for laboratory Investigation for hepatitis marker using the ELISA techniqu
Correlation between family history of Hepatitis B and positive HBV serologic tests in patients attending dental clinics in Riyadh
No full textThe aim of this study was to investigate the importance of family history in the identification of HBV sero positive subjects.
A total of 121 patients attending dental clinics were recruited for this study. Inquiry about family history of hepatitis, if any
was obtained from each subject. Subjects who reported family history of hepatitis or hepatitis-like conditions were called
and sent for laboratory investigation for hepatitis markers using the ELISA technique. Data from 116 subjects with age
range 3-60 years, mean 33.3 was analyzed. There were 74 (63.8%) males and 42 (36.2%) females. A great majority of
subjects 66 (56.8%) were in their second and third decades. Family history of hepatitis was positive for 106 (91.4%) of the
subjects. The correlation relationship of detection of HBsAg with the family history was found only marginally significant
chi-square (P=0.052). In conclusion, a positive family history of HBV infection necessitates serologic investigation to rule
out sub-clinical carrier state
NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover
No full textAbstract
Background
Most of the cells of the mammalian retina are terminally differentiated, and do not regenerate once fully developed. This implies that these cells have strict controls over their metabolic processes, including protein turnover. We report the use of metabolic labelling procedures and secondary ion mass spectrometry imaging to examine nitrogen turnover in retinal cells, with a focus on the outer nuclear layer, inner nuclear layer, and outer plexiform layer.
Results
We find that turnover can be observed in all cells imaged using NanoSIMS. However, the rate of turnover is not constant, but varies between different cellular types and cell regions. In the inner and outer nuclear layers, turnover rate is higher in the cytosol than in the nucleus of each cell. Turnover rates are also higher in the outer plexiform layer. An examination of retinal cells from mice that were isotopically labeled very early in embryonic development shows that proteins produced during this period can be found in all cells and cell regions up to 2 months after birth, even in regions of high turnover.
Conclusions
Our results indicate that turnover in retinal cells is a highly regulated process, with strict metabolic controls. We also observe that turnover is several-fold higher in the synaptic layer than in cell layers. Nevertheless, embryonic proteins can still be found in this layer 2 months after birth, suggesting that stable structures persist within the synapses, which remain to be determined.Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659Georg-August-Universität Göttingen (1018
P4I/O: Intent-Based Networking with P4
No full textSwitches that can be (re)programmed through the network programming language P4 are able to completely change – even while in the field – the way they process packets. While powerful, P4 code is inherently static, as it is written and installed to accommodate a particular network requirement. Writing new P4 code each time new requirements arise may be complex and limits our agility to deal with changes in network traffic and services.In this paper, we present P4I/O, a new approach to data- plane programmability based on the philosophy of Intent-Based Networking. P4I/O provides an intent-driven interface that can be used to install and/or remove P4 programs on the switches when needed and which is easy to use. In particular, to realize P4I/O, we (1) describe an extensible Intent Definition Language (IDL), (2) create a repository of P4 code templates, which are parsed and merged based on the intents, (3) provide a technique to realize the resulting P4 program in a programmable switch, while accommodating intent modifications at any time, and finally (4) implement a proof-of-concept to demonstrate that intent modifications can be done on-the-fly.Embedded System
L'aventure d’habiter sa langue. Lire et traduire Susana Thénon
No full textCette édition mise en ligne est présentée sans les annexes de traductions.This thesis examines the possibility of translating into french the poetry of Argentinian author Susana Thénon (1935-1991), whose complete works were published in Buenos Aires in 2001 under the title La Morada imposible. We hypothesize a favorable moment for this reception, linked to the evolution of the reception of Hispano-American literature in France and the valorization of foreign authoress: translator Antoine Berman speaks of a translational “kairos”. First, this reception would take the form of a translation into French. The first part of this article examines the challenges of translation, and the specific translation difficulties posed by Thénon’s poetics. The second part takes us on a reading tour of Thénon's work, in which we discover a difficulty in inhabiting the language of writing, felt as an “impossible dwelling”. Gradually, the poet opts for polyphony and discursive chaos. This evolution calls for great caution when translating. For this reason, the third, exploratory, part focuses on attempts to accurately render the multiple voices and words present in Thénon's poems. This thesis is also the story of a reading experience and an opening to writing, through poetic translation. The translations proposed in this thesis are personal and unpublished.Ce mémoire interroge la possibilité d’accueillir en français la poésie de l’autrice argentine Susana Thénon (1935-1991), dont les œuvres intégrales ont été publiées en 2001 à Buenos Aires, sous le titre La Morada imposible. Nous y faisons l’hypothèse d’un moment favorable pour cet accueil, lié à l’évolution de la réception des littératures hispanoaméricaines en France et à la valorisation des autrices étrangères : le traductologue Antoine Berman parle à ce sujet de « kairos » traductif. Cette réception se matérialiserait, en premier lieu, par une traduction des poèmes en français. La première partie s’interroge ainsi sur les enjeux de cette entreprise de traduction et les difficultés de traduction spécifiques que posent la poétique thénonienne. La seconde partie propose un parcours de lecture dans l’œuvre de Thénon : nous y lisons une difficulté à habiter la langue d’écriture, ressentie comme une « demeure impossible ». Progressivement, la poétesse fait alors le choix de la polyphonie et du chaos discursif. Cette évolution invite à la plus grande prudence lors de la traduction. Pour cette raison, la troisième partie, exploratoire, s’attache aux tentatives de rendre avec justesse les multiples voix et paroles présentes dans les poèmes de Thénon. Ce mémoire est aussi le récit d’une expérience de lecture et d’une ouverture à l’écriture, par le biais de la traduction poétique. Les traductions proposées dans ce mémoire sont personnelles et inédites
Using bacterial biomarkers to identify early indicators of cystic fibrosis pulmonary exacerbation onset
No full textAcute periods of pulmonary exacerbation are the single most important cause of morbidity in cystic fibrosis patients, and may be associated with a loss of lung function. Intervening prior to the onset of a substantially increased inflammatory response may limit the associated damage to the airways. While a number of biomarker assays based on inflammatory markers have been developed, providing useful and important measures of disease during these periods, such factors are typically only elevated once the process of exacerbation has been initiated. Identifying biomarkers that can predict the onset of pulmonary exacerbation at an early stage would provide an opportunity to intervene before the establishment of a substantial immune response, with major implications for the advancement of cystic fibrosis care. The precise triggers of pulmonary exacerbation remain to be determined; however, the majority of models relate to the activity of microbes present in the patient's lower airways of cystic fibrosis. Advances in diagnostic microbiology now allow for the examination of these complex systems at a level likely to identify factors on which biomarker assays can be based. In this article, we discuss key considerations in the design and testing of assays that could predict pulmonary exacerbations
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