278 research outputs found
Towards adualism: becoming and nihilism in Nietzsche's philosophy
No full textThis chapter argues that Nietzsche held two doctrines of becoming: one more radical, which he requires to fend off nihilism, and one much more moderate—the ontology of relations he develops under the label ‘will to power’. Based on the latter he develops what the author call his ‘adualistic’—neither monistic nor dualistic—practice of thought, a ‘simultaneity-thinking’ (Zugleich-Denken) that is no longer subject to nihilism. For Nietzsche’s belief in the reality of the threat of nihilism to be intelligible, the author attributes to Nietzsche at least three assumptions that underpin his entire project: (1) ‘what there is, is becoming (and not being)’, (2) ‘most (if not all) strongly believe in being’, and (3) nihilism is a function of the belief in being
Nietzsche’s critique of staticism: Introduction to Nietzsche on time and history
No full textWhy are we still intrigued by Nietzsche? What the author argues in this chapter is that this sustained interest stems from Nietzsche’s challenge to what we might call the ‘staticism’ inherent in our ordinary experience. ‘Staticism’ can be defined, roughly speaking, as the view that the world is a collection of enduring, re-identifiable objects that change only very gradually and according to determinate laws. This article claims that as long as human beings subscribe to the ‘staticist picture’ Nietzsche will remain of interest. First, the chapter discusses Nietzsche’s rejection of the remnants of staticism in Hegel and Schopenhauer (both of whom, he holds, remain fundamentally opposed to taking time and history seriously). Second, it briefly outlines why Nietzsche deems the belief in any variant of the staticist picture as problematic. Finally, it examines Nietzsche’s adualistic-dialetheic stance towards the staticist worldview
CaMKII inhibition reduces arrhythmogenic Ca2+ events in subendocardial cryoinjured rat living myocardial slices
No full textSpontaneous Ca2+ release (SCR) can cause triggered activity and initiate arrhythmias. Intrinsic transmural heterogeneities in Ca2+ handling and their propensity to disease remodeling may differentially modulate SCR throughout the left ventricular (LV) wall and cause transmural differences in arrhythmia susceptibility. Here, we aimed to dissect the effect of cardiac injury on SCR in different regions in the intact LV myocardium using cryoinjury on rat living myocardial slices (LMS). We studied SCR under proarrhythmic conditions using a fluorescent Ca2+ indicator and high-resolution imaging in LMS from the subendocardium (ENDO) and subepicardium (EPI). Cryoinjury caused structural remodeling, with loss in T-tubule density and an increased time of Ca2+ transients to peak after injury. In ENDO LMS, the Ca2+ transient amplitude and decay phase were reduced, while these were not affected in EPI LMS after cryoinjury. The frequency of spontaneous whole-slice contractions increased in ENDO LMS without affecting EPI LMS after injury. Cryoinjury caused an increase in foci that generates SCR in both ENDO and EPI LMS. In ENDO LMS, SCRs were more closely distributed and had reduced latencies after cryoinjury, whereas this was not affected in EPI LMS. Inhibition of CaMKII reduced the number, distribution, and latencies of SCR, as well as whole-slice contractions in ENDO LMS, but not in EPI LMS after cryoinjury. Furthermore, CaMKII inhibition did not affect the excitation-contraction coupling in cryoinjured ENDO or EPI LMS. In conclusion, we demonstrate increased arrhythmogenic susceptibility in the injured ENDO. Our findings show involvement of CaMKII and highlight the need for region-specific targeting in cardiac therapies.sponsorship: David A. Eisner served as editor. We thank Dr. Filippo Perbellini for technical advice on LMS preparation. E. Dries was funded by a postdoctoral fellowship Fund for Scientific Research Flanders and international mobility awards by Scientific Research Flanders and University of Leuven. I. Bardi was funded by a National Heart and Lung Institute studentship. R. NunezToldra was funded by British Heart Foundation project grant PG/20/8/34856. We also thank the BritishHeart Foundation Centre for Cardiac Regeneration at Imperial College London for financial support. The authors declare no competing financial interests. Author contributions: Experimental design: C.M. Terracciano and E. Dries. Conducting experiments: E. Dries, I. Bardi, R. NunezToldra, and B. Meijlink. All authors contributed to the manuscript preparation. All authors approved the manuscript. (postdoctoral fellowship Fund for Scientific Research Flanders, Scientific Research Flanders, University of Leuven, National Heart and Lung Institute studentship, British Heart Foundation|PG/20/8/34856, BritishHeart Foundation Centre for Cardiac Regeneration at Imperial College London)status: Publishe
The role of microdomains in regulating ryanodine receptors (RyRs) and the alterations after myocardial infarction in cardiomyocytes
No full textstatus: Publishe
Minocycline-conditioning brings surveying and reactive microglial cells to an alerted state according to their potassium channel profile
No full textMicroglial cells, the macrophages of the central nervous system (CNS), are key players during inflammatory processes in the CNS and are characterized by the expression of several types of voltage-gated potassium (Kv) channels, such as the delayed rectifying potassium (Kdr) current in reactive microglial cells. This Kdr current has been reported as a physiological marker for microglial activation and is an important target to control microglial activities. In this study we investigated whether minocycline, a known microglial activation inhibitor, was able to inhibit the functional expression of this Kdr current. Therefore, the I/V relationships and the function of the Kv channels of primary cultured rat microglial cells were measured using the whole-cell patch-clamp technique. The I/V profiles were compared in control and lipopolysaccharide (LPS)-activated (100 ng/ml) microglial cells in absence and presence of minocycline (400 µg/ml)
Minocycline-conditioning brings surveying and reactive microglial cells to an alerted state according to their potassium channel profile
No full textMicroglial cells, the macrophages of the central nervous system (CNS), are key players during inflammatory processes in the CNS and are characterized by the expression of several types of voltage-gated potassium (Kv) channels, such as the delayed rectifying potassium (Kdr) current in reactive microglial cells. This Kdr current has been reported as a physiological marker for microglial activation and is an important target to control microglial activities. In this study we investigated whether minocycline, a known microglial activation inhibitor, was able to inhibit the functional expression of this Kdr current. Therefore, the I/V relationships and the function of the Kv channels of primary cultured rat microglial cells were measured using the whole-cell patch-clamp technique. The I/V profiles were compared in control and lipopolysaccharide (LPS)-activated (100 ng/ml) microglial cells in absence and presence of minocycline (400 µg/ml)
Mining predictive k-CNF expressions
No full textWe adapt Mitchell's version space algorithm for mining k-CNF formulae. Advantages of this algorithm are that it runs in a single pass over the data, is conceptually simple, can be used for missing value prediction, and has interesting theoretical properties, while an empirical evaluation on classification tasks yields competitive predictive results.sponsorship: This work was supported by a Postdoc grant from the Research Foundation-Flanders. This work is partially supported by the GOA project 2008/08 Probabilistic Logic Learning. This work uses HPC resources http://ludit.kuleuven.be/hpc. Anton Dries was the corresponding author. (Research Foundation-Flanders, GOA|2008/08)status: Publishe
Single-component organic solar cells-Perspective on the importance of chemical precision in conjugated block copolymers
No full textThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. The authors acknowledge financial support from Hasselt University, the Research Foundation-Flanders (FWO Vlaanderen; projects W000620N, I006320N, and 1S99620N), and the European Research Council (ERC; grant agreement 864625)
Resuspended freeze-dried Nannochloropsis as a model laboratory system for concentrated fresh Nannochloropsis in ultrasound cell disruption experiments
No full textMicroalgae have rigid, complex cell walls hindering direct lipid extraction. Cell disruption techniques are used to rupture these cellular structures to increase lipid extraction. Researchers investigating the downstream processing of microalgae do not always have access to microalgal cultivation systems to generate large amounts of fresh microalgal biomass. Using resuspended freeze-dried microalgal biomass as a model laboratory system for concentrated fresh biomass during cell disruption experiments offers greater flexibility in experimental planning and omits investment costs of microalgal cultivation equipment. So far, it however remains unclear whether freeze-dried resuspended biomass can be used as a model laboratory system to represent concentrated fresh biomass during cell disruption and lipid extraction experiments. This paper thus evaluated the suitability of resuspended freeze-dried Nannochloropsis as a model laboratory system for concentrated fresh Nannochloropsis during cell disruption. Ultrasound assisted cell disruption was used as example cell disruption technique and lipid extraction efficiency and free fatty acid content were investigated. Tap water and 3% sodium chloride are both suitable resuspension media for the resuspension of freeze-dried Nannochloropsis. Resuspension duration should be limited (< 120 min) to prevent the formation of free fatty acids. The condition of the biomass (concentrated fresh, or resuspended freeze-dried) prior to ultrasound assisted cell disruption did not influence the resulting lipid extraction efficiency. Resuspended freeze-dried Nannochloropsis biomass in tap water or 3% sodium chloride can thus be used as a model laboratory system for fresh microalgal biomass during research on ultrasound assisted lipid extraction. The generalization of the results to other cultivation conditions, cell disruption techniques, components of interest or microalgal species should be carefully assessed.The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by Flanders’ Food and funded by Flanders Innovation and Entrepreneurship (VLAIO) through the cSBR
project EffSep (Grant number HBC.2019.0012)
A Retrospective Examination of a Successful Developmental Reviewing Process
No full textIn this essay, we describe a retrospective examination of a review process for a manuscript that was published in the Journal of Management Studies (JMS) in 2015 (Hannah &Robertson, 2015). The two authors of the essay are (a) the first author of the JMS manuscript, David Hannah, and (2)the JMS editor of that manuscript, Dries Faems. We originally engaged in this examination to prepare for a presentation at the Strategy Process Interest Group workshop on“The Process of Publishing Process Research” during the 2015 Strategic Management Society meeting in Denver, Colorado. We have written this essay with a goal of sharing our observations about this review process. Although we share some of the content of the original manuscript herein, we focus most of our attention on describing each step in the review process from the perspective of the author as well as the editor. We conclude by offering what we hope are usefuland generalizable lessons about the challenges that authors and editors face in the review process, how to navigate them, and how to systematically improve the overall review process.We begin in July 2013, with JMS submission P0431, titled, “Why do Employees put Confidential Information at Risk? CI Protection and Confidentiality Tension in High-Tech Employees.” The paper reported the findings of a qualitative, theory-elaborating study involving 55 semistructured interviews with the employees of two high-tech companies
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