68 research outputs found
Regulation de la lactase intestinale chez le rat adulte
No full textSIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc
Batch experiments on the removal of U(VI) ions in aqueous solutions by adsorption onto a natural clay surface
No full textThe efficiency of the clay from Bikougou deposit (Gabon) as adsorbent for removing U(VI) ions dissolved artificially in aqueous solutions has been studied. Batch experiments have been performed for that by varying pH, U(VI) ions solution concentration, ionic solution strength, clay dosage, interaction duration and temperature. The U(VI) ions uptake per unit mass of clay, increases with the increase in pH (2-7), U(VI) ions solution concentrations and temperature. It decreases with the increase in ionic solution strength and clay dosage. The adsorption isotherm is best described by Dubinin-Kaganer-Radushkevich isotherm model. The kinetics of the adsorption of U(VI) ions on clay surface follows the pseudo-second-order kinetic model and the interaction realized spontaneously is exothermic. The mean values of thermodynamic constants ?H°, ?S° and ?G° obtained at 308k are respectively-62.54kJ/mol, -0.18kJ/K.mol and -8.68 kJ/mol. Keywords: Adsorption capacity, adsorbent, U(VI) ions solution, isotherm model, distribution constan
Digestion of extracted <i>Loa loa</i> DNA by EcoRI and BamHI endonuclease.
No full text2A: Undigested (lines: 1-3-5-7-9-11) compared to digested with EcoRI (lines: 2-4-6-8-10-12) DNA; extracts were analyzed via agarose gel electrophoresis (1%) and visualized under UV light. The values on the left indicate the size of the bands. PM = molecular marker. 2B: Undigested (lines:1-3-5-7-9-11) compared to digested with BamHI (lines:2-4-6-8-10-12) DNA; extracts were analyzed via agarose gel electrophoresis (1%) and visualized under UV light. The values on the left indicate the size of the bands. PM = molecular marker.</p
Antiproliferative Effect Of Alcoholic Extracts Of Some Gabonese Medicinal Plants On Human Colonic Cancer Cells
No full textExtracts from Piptadeniastrum africanum Brenan (Mimosaceae),
Petersianthus macrocarpus (Breauv) L. (Lecydaceae), Cissus debilis
Planch (Vitaceae) and Dieffenbachia seguine Jacq. (Araceae) were
tested in vitro for their antiproliferative activity on human colon
cancer cell line (CaCo-2). The highest antiproliferative activities
were obtained with the alcoholic extracts of the roots of
Piptadeniastrum africanum (G-PAR), the leaves of Petersianthus
macrocarpus (G-PMF) and the stem of Cissus debilis (G-CDL), with 50%
inhibition concentrations (IC50) of 15 μg/ml, 17 μg/ml and 25
μg/ml respectively. Only one extract (leaves of Dieffenbachia
seguine (G-DSF)) exhibited weak antiproliferative activity with 50%
inhibition concentration (IC50) higher than 50 μg/ml
Amplification of DNA extracted by PCR.
No full textComparison of amplicon obtained after amplification of Loa loa DNA extracted with the six methods as template. The primers were designed from the Brugia ALT1 gene. Results of the analysis of amplicons after agarose gel electrophoresis (1.5%) and visualization under UV light. The methods are listed on the top of each band: phenol:chlorof line 1; Qiagen line 2; salting out line 3; Tris-EDTA line 4; Methanol line 5; CTAB line 6; band 7 is a negative control. PM = molecular marker. The values on the left represent the size of DNA.</p
Comparative analysis of spectrometric data from six DNA extraction methods.
No full textComparative analysis of spectrometric data from six DNA extraction methods.</p
Comparison of six methods of <i>Loa loa</i> DNA extraction.
No full textExtracted DNA in duplicate was analyzed by electrophoresis in a 0.8% agarose gel and visualized under UV light. PM = standard molecular weight. Lines 1–2: DNA from phenol/chloroform; 3–4: Qiagen extract; 5–6: Salting-out extract; 7–8: Tris-EDTA extract; 9–10: Methanol extract; 11–12: CTAB extract. The values on the left are the size of molecules.</p
S1 Raw images -
No full textFig 1. Comparison of six methods of Loa loa DNA extraction. PM = standard molecular weight. Lines 1–2: DNA from phenol/chloroform; 3–4: Qiagen extract; 5–6: salting-out extract; 7–8: Tris-EDTA extract; 9–10: methanol extract; 11–12: CTAB extract. The values on the left are the size of molecules. Fig 2. Digestion of extracted Loa loa DNA by EcoRI and BamHI endonuclease. 2A: Undigested (lines: 1-3-5-7-9-11) compared to digested with EcoRI (lines: 2-4-6-8-10-12) DNA; PM = molecular marker. 2B: Undigested (lines:1-3-5-7-9-11) compared to digested with BamHI (lines:2-4-6-8-10-12) DNA; extracts were analyzed via agarose gel electrophoresis (1%) and visualized under UV light. The values on the left indicate the size of the bands. PM = molecular marker. Fig 3. Amplification of DNA extracted by PCR. The methods are listed on the top of each band: phenol:chlorof line 1; Qiagen line 2; salting out line 3; Tris-EDTA line 4; Methanol line 5; CTAB line 6; band 7 is a negative control. PM = molecular marker. The values on the left represent the size of DNA. (PDF)</p
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