77 research outputs found
Anti-nociceptive and anti-inflammatory activities of crude root extract and solvent fractions of Cucumis ficifolius in mice model
No full textDesalegn Getnet Demsie,1,2 Ebrahim M Yimer,1 Abera Hadgu Berhe,1 Birhanetensay Masresha Altaye,3 Derbew Fikadu Berhe11Department of Pharmacology and Toxicology, College of Health Sciences, Mekelle University, Mekelle, Ethiopia; 2Department of Pharmacy, College of Medicine and Health Sciences, Adigrat University, Adigrat, Ethiopia; 3Department of Pharmacy, College of Medicine, Debre Berhan University, Debre Berhan, EthiopiaBackground: Societies in developing countries use traditional medicine as alternatives for management of pain and inflammation. The plant Cucumis ficifolius has been used in Ethiopia to treat many ailments including inflammation and pain. The objective of this study was to evaluate the antinociceptive and anti-inflammatory activities of the crude root extract and solvent fractions of C. ficifolius.Methods: The analgesic activity of crude extract and solvent fractions of C. ficifolius was evaluated with acetic acid-induced writhing, hot plate, and formalin-induced paw licking tests. The anti-inflammatory effect of crude methanolic root extract and solvent fractions of C. ficifolius was evaluated using carrageenan-induced paw edema. The crude extract was given at 200, 400 and 800 mg/kg. Butanol and aqueous fractions were given at 100 and 200 mg/kg doses. The negative control groups were treated with distilled water (10 mL/kg). Standard drugs used wereacetylsalicylic acid(ASA) in acetic acid, formalin tests and carrageenan-induced paw edema and morphine (20 mg/kg) in hot plate test.Results: The crude extract, at its maximum dose, produced comparable analgesic activity (72.5%) to ASA in acetic acid writhing test. In the hot plate test, both the crude extract and solvent fractions exhibited a significant prolongation of nociception reaction time. Formalin test result indicated a significant reduction of mean lick time with maximal protection of 64% (early phase) and 83% (late phase). Aqueous and butanol fractions showed good analgesic activity in the three models. Inflammation was decreased by 69% with butanol (200 mg/kg); 71% (800 mg/kg) of crude extract and by 41% and 56% with the use of aqueous fraction at 100 and 200 mg/kg, respectively (p<0.001).Conclusion: The present study indicates that the crude methanolic root extract, as well as butanol and aqueous solvent fractions, showed anti-nociceptive and anti-inflammatory activities.Keywords: hot plate test, writhing test, paw edema, formalin test, carrageenan, 80% methano
Netrin as a Novel Biomarker and Its Therapeutic Implications in Diabetes Mellitus and Diabetes-Associated Complications
No full textDiabetes is a multifactorial metabolic syndrome and is one of the shared long-lasting illnesses globally. It is linked to long-term microvascular and macrovascular complications that contribute to disability, compromised quality of life, and reduction in lifespan, which eventually leads to death. This disease is not only incurring significant economic burden but also adversely affects the patients, caregivers, communities, and the society at large. The interruption of diabetes progress and its complications is a primary focus of scientific communities. In spite of various diagnostic modalities for diabetes, there is a limited marker to investigate the risk and progress of its complications. Netrin has recently received more attention as a biomarker of diabetes and a broader range of long-term complication. Therefore, the impetus of this review is to exhaustively discuss the role of Netrin as a potential biomarker and its therapeutic implication in diabetes and diverse sets of microvascular and macrovascular complications of diabetes. It also discourses the possible mechanisms of Netrin for the said pharmacological effect for a better understanding of the development and progression of diabetes and its complications in relation to this protein. It enables protective measures to be applied at the subclinical stage and the responses to preventive or therapeutic measures to be scrutinized. Besides, it might also facilitate the appraisal of novel therapeutic options for diabetes and various complications through modifying the endogenous Netrin and provide surrogate endpoints for intervention
Repurposing of the β-Lactam Antibiotic, Ceftriaxone for Neurological Disorders: A Review
No full textTo date, there is no cure or disease-modifying agents available for most well-known neurological disorders. Current therapy is typically focused on relieving symptoms and supportive care in improving the quality of life of affected patients. Furthermore, the traditional de novo drug discovery technique is more challenging, particularly for neurological disorders. Therefore, the repurposing of existing drugs for these conditions is believed to be an efficient and dynamic approach that can substantially reduce the investments spent on drug development. Currently, there is emerging evidence that suggests the potential effect of a beta-lactam antibiotic, ceftriaxone (CEF), to alleviate the symptoms of different experimentally-induced neurological disorders: Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, epileptic-seizure, brain ischemia, traumatic brain injuries, and neuropathic pain. CEF also affects the markers of oxidative status and neuroinflammation, glutamatergic systems as well as various aggregated toxic proteins involved in the pathogenesis of different neurological disorders. Moreover, it was found that CEF administration to drug dependent animal models improved the withdrawal symptoms upon drug discontinuation. Thus, this review aimed to describe the effects of CEF against multiple models of neurological illnesses, drug dependency, and withdrawal. It also emphasizes the possible mechanisms of neuroprotective actions of CEF with respective neurological maladies
Integrated hydrogeological modelling of hard-rock semi-arid terrain: supporting sustainable agricultural groundwater use in Hout catchment, Limpopo Province, South Africa
No full textAn integrated hydrogeological modelling approach applicable to hard-rock aquifers in semi-arid data-scarce Africa was developed using remote sensing, rainfall-runoff modelling, and a three-dimensional (3D) dynamic model. The integrated modelling approach was applied to the Hout catchment, Limpopo Province, South Africa, an important agricultural region where groundwater abstraction for irrigation doubled during 1968–1986. Since the 1960s, groundwater levels in irrigated areas have displayed extended periods of decline with partial or full recovery in response to major decadal rainfall events or periods. The integrated dynamic 3D hydrogeological flow model, based on the One-Water Hydrologic Flow Model (MODFLOW-OWHM), helped to understand recharge and flow processes and inform water use and management. Irrigation abstraction was estimated based on irrigated crop area delineated using the Landsat Normalized Difference Vegetation Index (NDVI) and crop water requirements. Using groundwater level data, the model was calibrated (2008–2012) and validated (2013–2015). Estimated mean diffuse recharge (3.3 ± 2.5% of annual rainfall) compared well with estimates from the Precipitation Runoff Modelling System model. Recharge and groundwater storage showed significant inter-annual variability. The ephemeral river was found to be losing, with mean net flux to the aquifer (focused recharge) of ~1.1% of annual rainfall. The results indicate a delicate human-natural system reliant on the small but highly variable recharge, propagating through variable pumping to an even more variable storage, making the combined system vulnerable to climate and anthropogenic changes. The integrated modelling is fundamental for understanding spatio-temporal variability in key parameters required for managing the groundwater resource sustainably
Advancing digital agriculture for climate resilience and economic transformation in Malawi
No full textMalawi, a landlocked country in Southeastern Africa, is vulnerable to the impacts of climate change. Agriculture is the backbone of its economy, supporting more than 80% of the population, contributing 30% to the national Gross Domestic Product (GDP), and generating 80% of export earnings. The World Bank projects that, under a business-as-usual scenario, climate change could reduce Malawi’s GDP by
3–9% by 2030, 6–20% by 2040 and 8–16% by 2050. These losses are primarily attributed to infrastructure damages, including roads and bridges, and decreased labor productivity due to heat stress. Additionally, climate shocks could push an estimated two million people into poverty by 2030 and increase to four million by 2040. However, this critical sector is largely dominated by smallholder farmers who rely on rainfed systems and face persistent structural, technological, and climate-related challenges. As climate variability intensifies—bringing more frequent droughts, floods, and erratic rainfall—the urgency to adapt and innovate to ensure the resilience of the country’s agrifood system becomes paramount. In this
context, digital agriculture offers a transformative pathway to improve productivity, resilience, and inclusivity in the face of climate and economic uncertainty. This brief synthesizes findings from a study conducted by the International Water Management Institute (IWMI) and the Global Center on Adaptation (GCA). Drawing on literature reviews, field consultations, and surveys from districts including Lilongwe, Dowa, Zomba and Mchinji, the report evaluates the role of digital agriculture in climate adaptation, identifies barriers to adoption, and proposes strategic investments for inclusive agricultural transformation
Simulation-optimization approach for evaluating the feasibility of managed aquifer recharge in the Samail Lower Catchment, Oman
No full textThis article presents a simulation-optimization approach for evaluating the feasibility of managed aquifer recharge (MAR) in the Samail Lower Catchment, Oman. The objective is to provide a maximum recharge and extraction rate through MAR in an annual cycle of two successive injection and recovery periods, while meeting operational and system constraints such as water level, gradient, and travel time. Three groundwater management problems were solved by coupling a simulation model with successive linear programming (SLP) and the nondominated sorting genetic algorithm (NSGA-II) multiobjective genetic algorithm. Sensitivity analysis was also completed to examine the overall response of the simulation-optimization results to changes in hydraulic conductivities and maximum injection rates. Results using the SLP algorithm showed that the total volume of injected water for 4 months of injection without recovery is as high as 8 × 106 m3, and the total recovered volume of water for 4months injection and 8 months recovery is approximately 5.3 × 106 m3, giving a total recovery efficiency of approximately 66%. For the same setup the NSGA-II algorithm derived the entire nondominated front of solutions for two conflicting objectives: maximizing recovery rate and maximizing minimum groundwater head close to the sea (for preventing seawater intrusion). This algorithm includes travel time constraints directly in the optimization process. In conclusion, the proposed approach provides a cost-effective means to evaluate MAR in a coastal aquifer
The Effect of Metformin in Experimentally Induced Animal Models of Epileptic Seizure
No full textBackground. Epilepsy is one of the common neurological illnesses which affects millions of individuals globally. Although the majority of epileptic patients have a good response for the currently available antiepileptic drugs (AEDs), about 30-40% of epileptic patients are developing resistance. In addition to low safety profiles of most of existing AEDs, there is no AED available for curative or disease-modifying actions for epilepsy so far. Objectives. This systematic review is intended to evaluate the effect of metformin in acute and chronic animal models of an epileptic seizure. Methods. We searched PubMed, SCOPUS, Sciences Direct, and grey literature in order to explore articles published in English from January 2010 to November 2018, using key terms “epilepsy,” “seizure,” “metformin,” “oral hypoglycemic agents,” and “oral antidiabetic drugs”. The qualities of all the included articles were assessed according to the Collaborative Approach to Meta-Analysis and Review of Animal Data from Experimental Studies (CAMARADES). Results. Out of six hundred fifty original articles retrieved, eleven of them fulfilled the inclusion criteria and were included for final qualitative analysis. In these studies, metformin showed to control seizure attacks by attenuating seizure generation, delaying the onset of epilepsy, reducing hippocampal neuronal loss, and averting cognitive impairments in both acute and chronic models of an epileptic seizure. The possible mechanisms for its antiseizure or antiepileptic action might be due to activation of AMPK, antiapoptotic, antineuroinflammatory, and antioxidant properties, which possibly modify disease progression through affecting epileptogenesis. Conclusion. This review revealed the benefits of metformin in alleviating symptoms of epileptic seizure and modifying different cellular and molecular changes that affect the natural history of the disease in addition to its good safety profile
An assessment of groundwater use in irrigated agriculture using multi-spectral remote sensing
No full textHydrogeological characterization of crystalline aquifer in the Hout River Catchment, Limpopo Province, South Africa
No full textThis study attempted to conceptualize the hydrogeological setting of the Hout River Catchment, located in the Limpopo River Basin, using multiple methods that include groundwater flow patterns, structural analysis, stable (18O, 2H and 13C) and radiogenic (14C) isotopes of water and Water Table Fluctuation methods. The hydrogeological system of the catchment is represented by fractured crystalline basement aquifer as the main host for groundwater and is overlain by weathered rocks that act as a vadose zone and shallow aquifer in various places. Groundwater from the fractured basement rocks is the main source of water for large-scale irrigation and domestic use. Potential aquifers in the area are evident within the Hout River granitic gneiss and the Goudplaats granitic gneiss besides the younger granites as a result of fracturing and weathering. Groundwater flow map shows a flow pattern from the southern part of the catchment towards the north-eastern part of the catchment dictated by dolerite dykes and tectonic lineaments that trend in the ENE and E direction (088° and 075°) with the dip angle of 50° to 55°. The deeper aquifer in the southern and central part of the catchment contain old groundwater with high salinity due to long residence time. The stable isotopes further confirmed the limited possibility of local recharge, with rather dominance of regional groundwater circulation into the catchment. The northern part of the catchment seems to be receiving recent recharge with the groundwater of high 14C content derived from the mountains that border the catchment
Potential Effect of Hydroxychloroquine in Diabetes Mellitus: A Systematic Review on Preclinical and Clinical Trial Studies
No full textBackground. Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia. It affects millions of people globally. In spite of many antidiabetic drugs that are available, an adequate level of control remains challenging. Hydroxychloroquine is an immunomodulatory drug that has been used for the treatment of malaria and autoimmune diseases. There is an emerging evidence that suggests its beneficial effect against diabetes mellitus. Therefore, this systematic review is aimed at discoursing the role of hydroxychloroquine against diabetes mellitus and its potential mechanisms of actions. Methods. A systematic and manual searching was carried out to retrieve relevant articles (preclinical and clinical studies) published from January 2014 to July 2019. Electronic databases including PubMed and Scopus as well as clinicaltrials.gov have been searched using different searching terms: “hydroxychloroquine,” “diabetes mellitus,” “hyperglycemia,” and “insulin resistance.” The MeSH terms (PubMed) and text words were combined with “AND” or “OR.” In addition, manual searching of Google Engine and Google Scholar was conducted. Quality assessment of all the included studies was performed using CAMARADES (preclinical studies) and the Newcastle-Ottawa Scale and Cochrane Collaboration’s tools (clinical studies). Results. A total of eighteen studies (three experimental and fifteen clinical studies) were found to be eligible for the present systematic review. Among the included clinical studies (six randomized control trials, five observational studies, and four cohort studies), about 55,776 study participants were involved. Most of these studies showed significant improvement of lipid profile and insulin levels and substantial diminution of hemoglobin A1c, fasting plasma glucose, and postprandial blood glucose levels. Reduction in lysosomal degradation of the internal insulin-insulin receptor complex and enhancement in insulin sensitivity and adiponectin levels are some of the hypothesized mechanisms for the antidiabetic effect of hydroxychloroquine. Conclusion. The current review provides preliminary evidence for potential antidiabetic properties of hydroxychloroquine. Though the provided available data were promising, further clinical trials and mechanistic studies are needed to determine its long-term effects
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