1,721,059 research outputs found
Phase equilibria calculation by Group-contribution Perturbed Hard Sphere Chain Equation of state
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A comparison between semiempirical and molecular based equations of state for describing the thermodynamic of supercritical micronization processes.
No full textMETHOD FOR CELL REPROGRAMMING AND DIFFERENTIATION BY MICROFLUIDIC TECHNOLOGY
No full textA method for reprogramming differentiated cells and for then converting the reprogrammed cells into a differentiated phenotype of interest by means of microfluidic technology is described, together with a related kit for cell reprogramming
Microfluidics for secretome analysis under enhanced endogenous signaling
No full textCell secretome, the complex set of proteins that are secreted by the cells, is a fundamental mechanism of
cell-cell communication both in vitro and in vivo. In vivo, the analysis of proteins secreted into body fluids
can bring to the identification of biomarkers for important physiopathological conditions. However, due
to the complexity of the protein content of body fluids, a better understanding of the secreted proteins by
different cell types is highly desirable and can be performed in vitro for dissection. To this aim, microfluidic
culture systems could be particularly relevant because of the accumulation of extrinsic endogenous
signals at microliter scale, which better preserves the self-regulation occurring in the small
interstitial spaces in vivo. In this work, we perform a quantitative study to compare the secretome in
microfluidics and in a standard well plate. Human foreskin fibroblasts are used as a case study. This work
also represents an important technological advance in terms of feasibility of high-throughput quantitative
protein analyses in microfluidics
Mechanical mixing device for cell culture multiwells
No full textDispositivo per la miscelazione meccanica di colture
cellulari in sospensione contenute in una piastra di
coltura, comprendente: - un coperchio 10 destinato ad
essere amovibilmente associato ad una piastra di
coltura P per schermare il pozzetto o i pozzetti W di
quest’ultima limitando l’ingresso di solidi sospesi
nell’aria ambiente, e al contempo permettendo
l’ingresso di gas e umidità esterni; - almeno una
girante di miscelazione 20 per ciascun pozzetto W della
piastra di coltura P; - mezzi motori 30 collegabili
cinematicamente a ciascuna girante 20.
La girante 20 è rotazionalmente associata al coperchio
10 in corrispondenza della faccia di quest’ultimo
destinata ad essere rivolta verso detta piastra di
coltura P. Almeno il coperchio 10 e le giranti 20 sono
realizzate con materiali che possono essere sottoposti
a trattamenti di sterilizzazione e sono in grado di
resistere alle condizioni di umidità, temperatura e pH
che si creano in un incubatore biologic
Microfluidic technology enhances the potential of human pluripotent stem cells
No full textSince the discovery of human somatic cell reprogramming, human induced pluripotent stem cells (hiPSC)
have been increasingly recognized as the landmark for development of organs-on-chip. hiPSCs show a
remarkable plasticity that is related to their ability to promptly respond to the surrounding environment.
In vitro, the soluble culture microenvironment, with its critical balance between exogenous and cellsecreted
factors, plays a great role in inducing hiPSC response, for both preserving pluripotency and
controlling differentiation stages. Exploring the complexity of hiPSC microenvironment requires new
experimental tools, as a tight control is limited within conventional culture dishes. Microfluidic technology
is particularly attractive in hiPSC research because of its ability to mimic specific environmental
cues by accurate control of soluble factors with high spatiotemporal resolution and in a high-throughput
fashion. In this review, we highlight recent progress in hiPSC research enabled by microfluidic technology
as well as new emerging scenarios
The emergence of the circadian clock network in hiPSC-derived hepatocytes on chip
Full text linkThe circadian clock has paramount implications in physiology and pathology. Although the circadian clock has been widely investigated in adults, up to now very little is known about how circadian rhythms emerge during embryonic development. Some studies about the ontology of the circadian system are focused on the development of the central pacemaker, whereas there is still no agreement about the development of the circadian clock in peripheral tissues. Our work represents the first attempt at investigating the onset of peripheral circadian clocks in the liver, which has a central role in controlling several aspects of human physiology. We profile the emergence of the circadian genes during the transition from the initial state of human pluripotency to the final state of hepatic maturation. We demonstrate that circadian rhythmicity is absent in human pluripotent stem cells, and it arises gradually during the process of hepatic commitment. The clock genes expression reaches a peak at the hepatic progenitor stage. At this point o hiPSC-derived f differentiation the gene oscillations start to be observed with a period of 13 h and approaches 24 h in a later stage when the clock primary feedback loop starts working properly. At the end of differentiation, circadian rhythmicity appears, with genes of primary and secondary feedback loops in antiphase (CLOCK, BMAL1 and REV-ERBα) a sign that the system becomes to be functional
Determination of glucose metabolic fluxes in live myoblasts by microfluidic nanosensing and data analysis
No full textPolymer processing for biomedical applications by innovative and sustenaible technologies.
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