49 research outputs found
Inheritance of Radiation Induced Sterility in Males of the Oriental Fruit Moth, Grapholitha Molesta Busck., (Lepidoptera: Tortricidae)
No full textAbstract GENCHEV, N. P. and E. M. GENCHEVA, 2006. Inheritance of radiation induced sterility in males of the Oriental fruit moth, Grapholitha molesta Busck., (Lepidoptera:Tortricidae). Bulg. J. Agric. Sci, Results of studies on the inheritance of radiation induced sterility in males of Grapholitha molesta Busck. are reported. The partially sterilizing effect of gamma radiation on irradiated as pupae male parents is characterized with moderate increase in parallel with the dose increase. The adult emergence from irradiated pupae, longevity of moths and sex ratio were not affected by the applied radiation doses. Female moths are more radiosensitive than males. The highest level of inherited sterility in males of F 1 generation was established after irradiation of their parents with dosage of 125 Gy. However, some decrease of this effect was observed in the variants with doses of 175 Gy and 200 Gy. The transmission of inherited sterility from F 1 males to their F 2 descendants is a slighter, but still perceptable process. The highest levels of inherited sterility were registered in the variants with dosages of 125 Gy and 150 Gy, but somewhat reduced effect was recorded for doses of 100, 175 and 200 Gy.Progeny of F 1 and F 2 males OFM manifested sex ratios significantly skewed in favor of male moths. As it seems, these sex ratio shifts are results of a dose-dependent process
Identification of nucleotide variation of growth hormone gene in rabbit populations reared in Bulgaria
No full text[EN] Five rabbit populations of New Zealand White (NZW), Californian (CAL), crossbred NZW×GW and two generations of the synthetic population – SPF1 and SPF2 reared in Bulgaria were included in the present study with the aim of detecting the genetic variability of the growth hormone encoding gene (GH) via polymerase chain reaction with the restriction fragment length polymorphism analysis and direct sequencing. The targeted region of the rabbit GH gene was amplified and a fragment of a total of 231 bp was obtained in all studied populations. Allele identification was determined after enzymatic digestion, where two fragments of 62 and 169 bp correspond to allele C and an undigested fragment of 231 bp corresponds to allele T. Two additional bands of 107 and 124 bp evidenced A/G genetic polymorphism in the rabbit GH gene. Thirtyeight percent of the studied rabbits were carriers of the double mutation (C/T+A/G) in the same locus as the studied GH gene. The sequence analysis revealed two nucleotide substitutions – g.111C>T and g.156A>G in the non-coding region between the regulatory TATA box and 5’ UTR region, and a novel g.255G>A genetic variant in intron 1 of GH gene. The A>G transition was most frequent (40.57%), compared to the other ones, G>A (28.57%) and C>T (10.80%), respectively. The most frequent genotype in the NZW population was homozygous TT (0.93), with a prevalence of the T allele (0.97) over allele C (0.03) for g.111C>T SNP site. The distribution of the allele and genotype frequencies at the sites g.156A>G and g.255G>A in this rabbit group was identical, with the highest value of 0.93 for alleles A and G, respectively. The rabbit populations CAL and NZW×GW showed equal frequencies of the prevalent T allele (0.83) and for homozygous TT genotype (0.67) according to g.111C>T SNP. The highest values were obtained for the allele А (0.83) and for homozygous AA genotype (0.67) at c.33A>G SNP in these rabbit groups. The highest values (0.67, 0.60 and 0.80) for the heterozygous genotypes at g.111C>T, g.156A>G and g.255G>A SNPs, respectively, were detected among the SPF2 rabbit population, compared to the both homozygous genotypes. The results obtained in the present research indicates a significant degree of genetic variability of the studied polymorphic GH locus in the SPF2 rabbit group.Gencheva, DG.; Koynarski, TV.; Dafova, V.; Tanchev, SG. (2021). Identification of nucleotide variation of growth hormone gene in rabbit populations reared in Bulgaria. World Rabbit Science. 29(1):19-29. https://doi.org/10.4995/wrs.2021.12693OJS1929291Abdel-Kafy E., Hussein B., Abdel-Ghany S., El-Din A., Badawi Y. 2015. Single nucleotide polymorphisms in growth hormone gene are associated with some performance traits in rabbit. Int. J. Biol. Pharm. Allied Sci., 4: 490-504.Abdel-Kafy, E., Darwish, S., Elkhishin, D. 2016. Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit. World Rabbit Sci., 24: 213-221. https://doi.org/10.4995/wrs.2016.4026Amalianingsih T., Brahmantiyo B. 2014. The variability of growth hormone gene associated with ultrasound imaging of longissimus dorsi muscle and perirenal fat in rabbits. Media Peternakan, 37: 1-7. https://doi.org/10.5398/medpet.2014.37.1.1Dimitrova I., Dimitrov T., Teneva A., Tzvetkova H. 2008. Rabbit production in Bulgaria. Biotechnol. Anim. Husbandry, 24: 149-154. https://doi.org/10.2298/BAH0802149DEl-Sabrout K., Aggag S. A. 2017. Associations between single nucleotide polymorphisms in multiple candidate genes and body weight in rabbits. Vet. World, 10: 136. https://doi.org/10.14202/vetworld.2017.136-139El-Sabrout K., Aggag S., de Souza Jr J.B.F. 2019. Some recent applications of rabbit biotechnology - a review. Anim. Biotechnol., 1-5. https://doi.org/10.1080/10495398.2018.1539005Fontanesi L., Dall'Olio S., Spaccapaniccia E., Scotti E., Fornasini D., Frabetti A., Russo V. 2012. A single nucleotide polymorphism in the rabbit growth hormone (GH1) gene is associated with market weight in a commercial rabbit population. Livest. Sci., 147: 84-88. https://doi.org/10.1016/j.livsci.2012.04.006Fontanesi L., Tazzoli M., Scotti E., Russo V. 2008. Analysis of candidate genes for meat production traits in domestic rabbit breeds. In Proc.: 9th World Rabbit Congress, June 10-13, 2008, Verona, Italy, 79-84.Gencheva D., Georgieva S., Velikov K., Koynarski T., Tanchev S. 2017. Single nucleotide polymorphism of the Growth Hormone Receptor (GHR ) encoding gene in Oryctolagus cuniculus. J. BioSci. Biotechnol., 6: 197-201.Heracle BioSoft. 2013. DNA Sequence Assembler v.4. https://www.DnaBaser.comHristova D.G., Tanchev S.G., Velikov K.P., Gonchev P.G., Georgieva S.J. 2018. Single nucleotide polymorphism of the growth hormone (GH ) encoding gene in inbred and outbred domestic rabbits. World Rabbit Sci., 26: 49-55. https://doi.org/10.4995/wrs.2018.7211Hristova D., Tanchev S., Velikov K., Gonchev P., Georgieva S. 2017. Rabbit growth hormone and myostatin gene polymorphisms. J. Agr. Res., 2: 000133. https://doi.org/10.23880/OAJAR-16000133Hussein B., Abdel-Kafy E.M., Abdel-Ghany S.M., Gamal A.Y., Badawi Y.M. 2015. Single nucleotide polymorphism in growth hormone gene are associated with some performance traits in rabbit. Int. J. Biol. Pharm. Allied Sci., 4: 490-504.Kumar S., Stecher G., Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 33: 1870-1874. https://doi.org/10.1093/molbev/msw054Migdal L., Palka S., Kmiecik M., Derewicka O. 2019. Association of polymorphisms in the GH and GHR genes with growth and carcass traits in rabbits (Oryctolagus cuniculus). Czech J. Anim. 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Nucleic Acids Res., 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673Wallis C., Wallis M. 1995. Cloning and characterisation of the rabbit growth hormone-encoding gene. Gene, 163: 253-256. https://doi.org/10.1016/0378-1119(95)00429-AYeh F., Yong R. 1999. POPGENE version 1.31 (02.04.2011). Microsoft based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada, http://www.ualberta.ca/~fyeh/fyehZaghloul A. R.; Khalil, M.H.; Iraqi, M. M.; Ramadan, Sh. and EL Nagar, A. G. 2019. Crossbreeding effects and polymorphic associations of genotypes of GH gene with growth traits in rabbits. Egyptian Journal of Rabbit Science, 29: 149-169. https://doi.org/10.21608/ejrs.2019.81100Zhang W.X., Zhang G.W., Peng J., Lai S.J. 2012. The polymorphism of GHR gene associated with the growth and carcass traits in three rabbit breeds. In Proc.: 10th World Rabbit Congress, Sharm El-Sheikh, Egypt, 75-78
Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits
No full text[EN] The objective of the present study was to evaluate the influence of the genotypes of two single nucleotide polymorphisms (SNPs) c.78C>T located in the growth hormone gene (GH) and c.106C>G in the growth hormone receptor gene (GHR) on individual body weight (IBW) during the growing period at 35, 70 and 90 d of age on a total of 107 weaned Californian breed rabbits. The restriction fragments obtained revealed that 74.8% of the rabbits carrying c.78C>T SNP and 52.3% of the rabbits carrying c.106C>G SNP were heterozygous, which indicated a moderate level of genetic diversity in this Californian population. Association analysis based on a single-gene approach revealed that c.78C>T polymorphism in the GH gene had a significant effect (P<0.05) on the weight at 70 and 90 d of age. The highest IBW (2530.4±66.6 g) was observed in rabbits carrying the c.78C>T TT genotype, and detected individuals were significantly affected by the dominance effect. Significant differences were observed between individuals with homozygous c.106C>G CC genotype and those with heterozygous CG genotype. The highest IBW (2462.0±198.3 g) was observed in rabbits carrying the c.106C>G CC genotype and detected individuals were significantly affected by the additive effect. A total of nine combined genotypes of c.78C>T and c.106C>G SNPs was found in the study, of which only four major groups (CT/CC, CC/CG, CT/CG, and CT/GG) were concerned in the diplotype analysis. Significant differences were observed between individuals with CT/CC and CC/CG genotype combinations, and between those with the CC/CG and CT/GG diplotypes. However, the highest IBW at 90 d of age (2447.2±213.8 g) was observed in rabbits carrying the CT/CC genotype combinations. The highest coefficient of determination found for individual body weight at 90 d of age (R2=10.8%) indicated a high effect of genotype combinations. In conclusion, the results obtained suggested that c.78C>T of GH gene and c.106C>G of GHR gene could be useful candidate genes to improve growth performance in Californian rabbits with potential application in rabbit breeding programmes.Gencheva, DG.; Velikov, KP.; Veleva, PM. (2022). Association analysis of nucleotide polymorphisms in growth hormone (GH) and its receptor (GHR) with body weight in Californian rabbits. World Rabbit Science. 30(1):95-102. https://doi.org/10.4995/wrs.2022.13127OJS95102301Bai W.L., Zhou C.Y., Ren Y., Yin R.H., Jiang W.Q., Zhao S.J., Zhang S.C., Zhang B.L., Luo G.B., Zhao Z.H. 2011. Characterization of the GHR gene genetic variation in Chinese indigenous goat breeds. Mol Biol Rep., 38: 471-479. https://doi.org/10.1007/s11033-010-0130-2Baselga M. 2004. Genetic improvement of meat rabbits. Programmes and diffusion. In Proc.: 8th World Rabbit Congress, September 7-10, 2004, Pueblo, Mexico, 1-13.Dekkers J.C. 2004. Commercial application of marker- and geneassisted selection in livestock: Strategies and lessons, J. Anim. Sci., 82, 13: 313-328.Deng X.S., Wan J., Chen S.Y., Wang Y., Lai S.J., Jiang M.S., Xu M. 2008. The correlations between polymorphism of growth hormone receptor gene and butcher traits in rabbit. Yi chuan. Hereditas, 30: 1427-1432. https://doi.org/10.3724/SP.J.1005.2008.01427Dimitrova I., Dimitrov T., Teneva A., Tzvetkova H. 2008. Rabbit production in Bulgaria. Biotech. Anim. Husbandry, 24: 1-2: 149-154. https://doi.org/10.2298/BAH0802149DEASRAB. Livestock Breeds in the Republic of Bulgaria Executive Agency for Selection and Reproduction in Animal Breeding, Catalogue, 5th ed. In: Nikolov V. (editor), Sofia, Bulgaria; 2017. pp. 80-81.El-Sabrout K., Aggag S.A. 2017. Associations between single nucleotide polymorphisms in multiple candidate genes and body weight in rabbits. Vet. World, 10: 136-139. https://doi.org/10.14202/vetworld.2017.136-139Fontanesi L., Dall’Olio S., Spaccapaniccia E., Scotti E., Fornasini D., Frabetti A., Russo V. 2012. Asingle nucleotide polymorphism in the rabbit growth hormone (GH1) gene is associated with market weight in a commercial rabbit population. Livest. Sci., 147: 84-88. https://doi.org/10.1016/j.livsci.2012.04.006Fontanesi L., Sparacino G., Utzeria V.J., Scottia E., Fornasini D., Dall’Olioa S., Frabetti A. 2016. Identification of Polymorphisms in the Rabbit Growth Hormone Receptor (GHR) Gene and Association with Finishing Weight in a Commercial Meat Rabbit Line. Anim. Biotechnol., 27: 77-83. https://doi.org/10.1080/10495398.2015.1101697Fontanesi L., Tazzoli M., Scotti E., Russo V. 2008. Analysis of candidate genes for meat production traits in domestic rabbit breeds. In Proc.: 9th World Rabbit Congress, June 10-13, 2008, Verona, Italy, 79-84.Frank S.J. 2001. Growth hormone signalling and its regulation: preventing too much of a good thing. Growth Horm IGF. Res., 11: 201-212. https://doi.org/10.1054/ghir.2001.0237Gencheva D., Georgieva S., Velikov K., Koynarski T., Tanchev S. 2017. Single nucleotide polymorphism of the Growth Hormone Receptor (GHR) encoding gene in Oryctolagus cuniculus. J. BioSci. Biotechnol., 6: 197-201.Grigorov I. 2005. How to grow rabbits. Zemizdat, Sofia. Helal M.M. 2019. Association between growth hormone receptor gene polymorphism and body weight in growing rabbits. Adv. Anim. Vet. Sci., 7: 994-998. https://doi.org/10.17582/journal.aavs/2019/7.11.994.998Herrington J., Carter-Su C. 2001. Signaling pathways activated by the growth hormone receptor. Trends Endocrinol Metab., 1: 252-257. https://doi.org/10.1016/S1043-2760(01)00423-4Hristova D., Tanchev S., Velikov K., Gonchev P., Georgieva S. 2017. Rabbit Growth Hormone and Myostatin Gene Polymorphisms. J. Agri. Res., 2: 1-6. https://doi.org/10.23880/OAJAR-16000133Hristova D.G., Tanchev S.G., Velikov K.P., Gonchev P.G., Georgieva S.J. 2018. Single nucleotide polymorphism of the growth hormone (GH) encoding gene in inbred and outbred domestic rabbits. World Rabbit Sci., 26, 1: 49-55. https://doi.org/10.4995/wrs.2018.7211Hussein B., Abdel-Kafy E.M., Abdel-Ghany S.M., Gamal A.Y., Badawi Y.M. 2015. Single nucleotide polymorphism in growth hormone gene are associated with some performance traits in rabbit. Int. J. Biol. Pharm. Allied Sci., 4: 490-504. Labate J. 2000. Software for population genetic analyses of molecular marker data. Crop Sci., 40: 1521-1528. https://doi.org/10.2135/cropsci2000.4061521xLeung D.W. Spencer S.A., Cachianes G., Hammonds R.G., Collins C., Henzel W.J., Barnard, R., Waters M.J., Wood W.I. 1987. Growth hormone receptor and serum binding protein: purification, cloning and expression. Nature, 330: 537-543. https://doi.org/10.1038/330537a0Marinov B., Grigorov I., Gurov B., Peshev R. 2009. Raising rabbits for meat. Sofia, pp. 1-334. Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. U.S.A. 70: 3321-3323. https://doi.org/10.1073/pnas.70.12.3321Polasik D., Kmiec M., Liefers S.,Terman A. 2005. Single nucleotide polymorphisms in exon 10 of the chinchilla growth hormone receptor (GHR) gene. J. Appl Genet. 46: 403-406.Ramadan S., Manaa E., El-Attrony M., EL Nagar A. 2020. Association of growth hormone (GH), insulin-like growth factor 2 (IGF2) and progesterone receptor (PGR) genes with some productive traits in Gabali rabbits. World Rabbit Sci., 28: 135-144.https://doi.org/10.4995/wrs.2020.12610Ruane J., Colleau J. J. 1996. Marker-assisted selection for a sex-limited character in a nucleus breeding population. J. 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Research of the Emotianal Inteligence of Bulgarian Physiotherapy Students
No full textEmotional intelligence is perceived as cognitive ability, social competence or personal disposition. It is a range of emotional competencies which determine the efficiency of human behavior. The aim of the research was to establish the level of emotional intelligence of Bulgarian physiotherapy students and to compare it with the average values for the population. The subject of the research was a total of 50 students with major kinesitherapy who study at the National Sports Academy with mean age 23.5 years(S= 3,66): 23 men and 27 women with mean age 22.96 years (S=3,24) and 24 years (S=3,96) respectively.We used Emotional Intelligence Questionnaire of Schutte, N. S., Malouff, J. M., Hall, L. E., Haggerty, D. J., Cooper, J. T., Golden, C. J. & L. Dornheim. (1998). The SEIS consists of 33 items responded to on a 5-point Likert Scale.The possible range of scoresis from 33 to 165. This questionnaire was translated into Bulgarian in 2006 and adapted for Bulgarian conditions by StoyanovaS. (2010) with a sample consisting mainly of students (N=724). It allows to be interpreted as emotional intelligence both as a general construct and as different components of the construct. Results The physiotherapy students, included in this pilot research, show a little over average levels of emotional intelligence measured with the questionnaire of Schutte,еtal (1998). With the advancement in age and experience the women show a little higher level of emotional intelligence. The Bulgarian version of the emotional intelligence scale of Schutte can be used for determining the level of emotional intelligence of students so that the level of their performance at university as well as their future professional realization can be prognosticated. Conclusion The higher emotional intelligence will help the future physiotherapeutic specialists to fulfill successfully their personal potential. They will have a positive social attitude and good ability for adaptation in the community environment. They will also be able to build healthy relationships with their patients which is a major factor for their motivation to actively take part in the healing process. DOI: 10.7176/JMPB/52-0
Complex formation of monomeric and dimeric copper(II) complexes with creatinine in organic media
No full textInfluence of Yoga Practices on Stress Coping Strategies
No full textStress coping strategies are used every day. By choosing a different coping style, people seek solution to a complicated task or situation. One of the reliable forms of motor activity, which influences favorably the development of valuable psychic and physical qualities, is yoga. The aim of the research is to determine the positive effect of yoga practices on stress coping strategies. The research was done among 76 women - 42 of them practice yoga in different clubs of the Bulgarian Yoga Federation and yoga students at NSA “Vassil Levski” – experimental group; and control group consisting of 34 women not engaged in any kind of sport or motor activity. The stress coping strategies were evaluated with the questionnaire СОРЕ-1, (Carver, C, & Scheier, M. 1985), adapted for Bulgarian conditions by A. Rusinova-Hristova and G. Karastoyanov (2000). Results show that the first seven stress coping strategies have approximately equal values with the control group. The passive strategies, related to emotion-focused coping with the problem, are dominant. In this case, the structure of the stress coping strategies with the women not engaged in sport is more imperfect than the one with the women practicing yoga. The arrangement of the leading stress coping strategies, as well as the reliable differences with six of the strategies (43% of all 14 strategies) in favor of the EG is indicative of more harmonious and more perfect structure of the preferred stress coping strategies with women practicing yoga. Conclusion: The leading stress coping strategies with women not engaged in sport are passive. They experience greater difficulty in solving their problems in stressful situations. The leading strategies with women practicing yoga are active. The researched individuals react immediately in stressful situations, concentrate on the problem and actively cope with it. Keywords: Stress coping strategies, yoga, motor activity
Mechanistic Control of Carcinoembryonic Antigen-related Cell Adhesion Molecule-1 (CEACAM1) Splice Isoforms by the Heterogeneous Nuclear Ribonuclear Proteins hnRNP L, hnRNP A1, and hnRNP M
No full textAbsolute structure of a new paramagnetic platinum(II)-creatinine complex with a columnar structure
No full textMicrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping
No full textWe have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with either chicken or frog histones, were separately digested to mononucleosomes using either micrococcal nuclease (MNase) or caspase-activated DNase (CAD). Both enzymes preferentially cleave internucleosomal (linker) DNA, although they do so by markedly different mechanisms. MNase has hitherto been very widely used to map nucleosomes, although concerns have been raised over its potential to introduce bias. Having identified the locations and quantified the strength of both the chicken or frog histone octamer binding sites on each DNA, the results obtained with the two enzymes were compared using a variety of criteria. Both enzymes displayed sequence specificity in their preferred cleavage sites, although the nature of this selectivity was distinct for the two enzymes. In addition, nucleosomes produced by CAD nuclease are 8-10 bp longer than those produced with MNase, with the CAD cleavage sites tending to be 4-5 bp further out from the nucleosomal dyad than the corresponding MNase cleavage sites. Despite these notable differences in cleavage behaviour, the two nucleases identified essentially equivalent patterns of nucleosome positioning sites on each of the DNAs tested, an observation that was independent of the histone type. These results indicate that biases in nucleosome positioning data collected using MNase are, under our conditions, not significant. (C) 2012 Elsevier Ltd. All rights reserved.</p
