1,721,659 research outputs found

    Dustin Michael Daniels gravemarker and fall decorations, hanging chimes, Logan, Utah, 1999 (1 of 2)

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    Dustin Michael Daniels gravemarker decorations in the Logan, Utah, Cemetery (1000 North 1200 East). Image is a close-up of a wind chime hanging from a metal hook. Also visible is an arrangement of dried branches in the fixed vase next to the stone

    Dustin Michael Daniels gravemarker and fall decorations, pumpkin and solar light, Logan, Utah, 1999

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    Close up of Dustin Michael Daniels gravemarker and its decorations in the Logan Cemetery (1000 North 1200 East) Logan, Utah. The closeup focuses on a pumpkin placed next to the headstone, a solar light, and a small American flag stuck into the ground. There is also a 94 high school tassle looped around the base of the fixed vase

    Dustin Michael Daniels gravemarker and fall decorations, graduation tassel, Logan, Utah, 1999 (1 of 2)

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    Closeup of Dustin Michael Daniels gravemarker decoations in the Logan, Utah, Cemetery (1000 North 1200 East). A '94' graduation tassel has been tied to one of the flower vases to the side of the headstone. Part of a pumpkin is also visible

    Dustin Michael Daniels gravemarker and fall decorations, engraved portrait, Logan, Utah, 1999

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    Close up of Dustin Michael Daniels portrait engraved on a gravemarker in the Logan Cemetery (1000 North 1200 East, Logan, Utah). Also visible is part of a pumpkin set next to the corner of the stone, and a '94 graduation tassel looped around one of the flower vases on the gravestone base

    Dustin Michael Daniels gravemarker and fall decorations, graduation tassel, Logan, Utah, 1999 (2 of 2)

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    Closeup of Dustin Michael Daniels gravemarker decoations in the Logan, Utah, Cemetery (1000 North 1200 East). A '94' graduation tassel has been tied to one of the flower vases to the side of the headstone. Part of a pumpkin is also visible

    Dustin Michael Daniels gravemarker and fall decorations, back view, Logan, Utah, 1999

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    Dustin Michael Daniels gravemarker and decorations in the Logan, Utah, Cemetery (1000 North 1200 East). The image shows the back of the headstone and the associated decorations placed around the stone. The back of the stone is engraved with the epitaph: 'DUSTIN The heartache and loneliness linger, but so do the memories of your warm laughter, mischievous grin, enthusiastic support, compassionate spirit, and tender love. A vibrant young man tragically plucked from the earth in full bloom, you will never be replaced or forgotten, only loved and remembered forever.' Various autumn decorations, including a decorative fence of Thanksgiving shapes, a wreath made of ripe wheat, a set of wind chimes, and autumn flower arrangements have been placed around the stone. Also visible are two solar lights on either side of the grave

    Dustin Michael Daniels gravemarker and fall decorations, front view, Logan, Utah, 1999 (2 of 2)

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    Dustin Michael Daniels gravemarker and decorations in the Logan, Utah, Cemetery (1000 North 1200 East). The image shows the headstone with its engraved portrait of Daniels, and the associated decorations placed around the stone. Both flower vases are filled with dried and artificial autumn flowers and branches. Another arrangement of artificial flowers in a basket has been set to the side of the stone, and a pumpkin has placed on the other side. To the left of the stone is a decorative Thanksgiving decoration made of three pieces of wood carved and painted to resemble a pilgrim holding a pumpkin, a cornstalk with yellow corncobs, and a turkey. Two solar lights are also visible on either side of the grave

    Dustin Michael Daniels gravemarker and Winter decorations, Logan, Utah, 2000 (1 of 2)

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    Dustin Michael Daniels gravemarker and decorations in the Logan, Utah, Cemetery (1000 North 1200 East). The image shows the back of the headstone and associated decorations. Both flower vases have an arrangement of dried flowers and branches and artificial leaves, plus bird ornaments, including a duck whirligig mounted on a wire. A tall hook holds a set of metal wind chimes, and a large evergreen wreath sits on a stand. Two solar lights have been placed to either side of the stone. The back of the stone is engraved with the epitaph: 'DUSTIN The heartache and loneliness linger, but so do the memories of your warm laughter, mischievous grin, enthusiastic support, compassionate spirit, and tender love. A vibrant young man tragically plucked from the earth in full bloom, you will never be replaced or forgotten, only loved and remembered forever.

    The immune synapse: Past, present, and future

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    Immunological synapses are specialized cell-cell junctions characterized by (1) close apposition of the immune cell membrane with the membrane of another cell driven by adaptive or innate immune recognition, (2) adhesion, (3) stability, and (4) directed secretion. This phenomenon was first recognized in the 1970s and the early 1980s through electron microscopy of ex vivo functioning immune cells. Progressive advances in fluorescence microscopy and molecular immunology in the past 20 years have led to rapid progress on understanding the modes of cell-cell interaction and underlying molecular events. This volume contains a diverse range of protocols that can be applied to the study of the immunological synapses and related immune cell junctions both in vitro and in vivo; and in disease settings in animal models and humans. We have also included chapters on critical molecular tools such as protein expression and mRNA electroporation that underpin or expand imaging approaches, although they are not specific to the study of immune synapses. We hope that these chapters will be of use to people entering the field as well as seasoned practitioners looking to expand their repertoire of methods
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