90 research outputs found
Assessing Endangered Felid Puma concolor Sperm Fertility by In Vitro Fertilization with Domestic Cat Oocytes
No full textThe Puma concolor population has been decreasing during the last 30 years. Semen cryopreservation of this species has been accomplished successfully and offers the possibility of preserving endangered species. We previously showed that fertilizing capability of wild felid spermatozoa can be evaluated using intracytoplasmic sperm injection (ICSI) with in vitro-matured domestic cat oocytes (Moro et al. 2014 Reprod. Domest. Anim. 49, 693-700). Due to the lack of homologous oocytes, we evaluated the capability of the Puma concolor sperm to induce domestic cat oocyte fertilization and subsequent pre-implantation embryo development. In the present study, cryopreserved sperm obtained by electroejaculation from five different males were used for IVF of in vitro-matured (IVM) domestic cat oocytes. Straws were thawed by exposing them to air for 10 s and then immersing in a 37°C water bath for 30 s. The contents of the straws were poured into a sterile 1.5-mL microtube pre-warmed to 37°C. The sperm suspension was diluted (1:3 v/v) by the slow (drop-by-drop) addition of a modified Tyrode’s solution. For IVF, IVM oocytes (n = 370) were co-incubated with 0.5 × 105 motile spermatozoa mL−1 in an atmosphere of 21% O2 in air at 38.5°C for 18 to 20 h. Presumptive zygotes were cultured in vitro in 50-μL drops of modified Tyrode’s medium on 6.5% CO2 in air at 38.5°C. Cleavage was determined at 48 h post-fertilization, and 5% FBS was added at Day 5 of in vitro culture. Blastocyst stage was evaluated at Day 8. Results (mean ± SEM) showed a high cleavage rate (179/370, 49.0 ± 4.0%), and a high development to morula stage (137/370, 34.4 ± 7.2%), and to blastocyst stage (94/370, 23.4 ± 4.7%) for all males. These results indicated that Puma concolor spermatozoa can induce domestic cat oocyte activation and development to blastocyst stage in similar rates to domestic cat homologous IVF: IVM oocytes (n = 291), cleavage rate (199/291, 67.1 ± 6.1%), development to morula stage (144/291, 47.8 ± 4.9%), and to blastocyst stage (86/291, 30.1 ± 1.6%). In conclusion, we demonstrated that domestic cat oocyte can be used to evaluated cryopreserve sperm samples from another felid species.Fil: Duque Rodriguez, Matteo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Sestelo, A.. Ecoparque Interactivo de Buenos Aires; ArgentinaFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
No full textSeveral equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling pathway during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic.Fil: Gambini, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Duque Rodriguez, Matteo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; ArgentinaFil: Rodriguez, Maria Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; ArgentinaFil: Briski, Olinda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; ArgentinaFil: Flores Bragulat, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Demergassi, Natalia. Fundación Temaikén; ArgentinaFil: Losinno, Luis. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Fisiología Animal; Argentin
Canine IVM With SOF Medium, Insulin-Transferrin-Selenium, and Low O2 Tension Improves Oocyte Meiotic Competence and Decreases Reactive Oxygen Species Levels
No full textAssisted reproductive technologies in canine species are limited due to the low efficiency of in vitro maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48–72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA–2.5% fetal bovine serum (FBS) and TCM-199 with 10% FBS. Additionally, we evaluated the effect of supplementation with insulin-transferrin-selenium (ITS) and low O2 tension in oocyte maturation, reactive oxygen species (ROS) levels, membrane integrity, and embryo development following parthenogenetic activation (PA). After 72 h of culture, SOF + BSA, SOF + BSA + FBS, and TCM-199 + FBS show 5, 7, and 4% of MII, respectively, without a statistical difference. However, SOF + BSA produced significantly higher degeneration rates compared to SOF + BSA + FBS (44 and 23%, respectively). Remarkably, supplementation with 1 μl/ml of ITS under high O2 tension demonstrated a beneficial effect by improving maturation rates up to 20% compared to the other groups. Low O2 tension increased maturation rates to 36.5%, although there were no statistical differences compared to high O2 tension in the presence of ITS. Lower ROS levels and higher integrity of the cytoplasmic membrane were found in the presence of ITS despite no differences in maturation rates under low O2 tension groups. Additionally, after PA, 1% development until the eight-cell stage was obtained after activation of in vitro-matured oocytes in the presence of ITS. Taken together, these results indicate that SOF supplemented with 8% BSA and 2.5% FBS is suitable for IVM of canine oocytes and ITS supplementation was beneficial for both high and low O2 tension. Furthermore, the addition of ITS in the cultured system lowers ROS levels and increases membrane integrity in domestic dog oocytes after IVM.Fil: Duque Rodriguez, Matteo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Cittadini, Camila O.. Universidad de Buenos Aires; ArgentinaFil: Teplitz, Gabriela Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: De Stefano, Adrian M.. Universidad de Buenos Aires; ArgentinaFil: Lombardo, Daniel M.. Universidad de Buenos Aires; ArgentinaFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentin
Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres
No full textHeterospecific embryo transfer of an endangered species has been carried out using recipients from related domestic females. Aggregation of an embryo from an endangered species with a tetraploid embryo from the species to be transferred could improve the development of pregnancy to term. The main objective of the present study was to analyze embryo aggregation in domestic cat model using hybrid embryos. For this purpose, we compared in vitro development of synchronic (Sync) or asynchronic (Async) and asynchronic with a tetraploid (Async4n) aggregation of domestic cat IVF embryos. Furthermore, aggregated blastocyst quality was analyzed by evaluation of the total cell number, cell allocation by mitotrackers staining of embryonic cells, expression of Oct4, Nanog, Sox2, Cdx2 genes, number of OCT4+ nuclei, and presence of DNA fragmentation. Additionally, the developmental rates of Async4n aggregation of domestic cat with Leopardus geoffroyi hybrid (hLg) embryos were evaluated. Async aggregation increased blastocyst cell number and the number of OCT4+ nuclei as compared to non-aggregated diploid (2n) and tetraploid (4n) embryos. Moreover, blastocysts produced by Async4n aggregation showed reduced rates of fragmented DNA. No differences were found in the expression of the pluripotent genes, with exception of the Cdx2 expression, which was higher in 4n and aggregated embryos as compared to the control group. Interestingly, hybrids embryos derived by Async4n aggregation with domestic cat embryos had similar rates of blastocysts development as the control. Altogether, the findings support the use of two-cell-fused embryos to generate tetraploid blastomeres and demonstrate that Async4n aggregation generates good quality embryos.Fil: Duque Rodriguez, Matteo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; ArgentinaFil: Gambini, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; ArgentinaFil: Ratner, Laura Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Sestelo, Adrian J.. Gobierno de la Ciudad de Buenos Aires. Secretaría de Ambiente. Ecoparque Interactivo; ArgentinaFil: Briski, Olinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Gutnisky, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Rulli, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Martin Fernández, Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; ArgentinaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentin
Supersonic Axial & Radial Rotor Design for ORC Applications: Using Method of Characteristics
No full textThe growing environmental concerns has put a lot of political & ethical pressure on the conventional industrial practices. The research community is ever so eager to investigate new technologies that can offer an efficient and environmentally sound solution to the issues caused by the polluting industrial processes. One of the potential solution is an Organic Rankine Cycle (ORC) which is proven to be an effective technology to efficiently extract energy from low-temperature sources. The ORC is basically a Rankine cycle but employs a high molecular weight organic fluid as the energy carrier. The peculiar non-classical gas dynamics behaviour of these fluids during dry expansion close to the vapour saturation line (in the dense gas region) poses certain challenges on the turbine design. Furthermore, the low speed of sound in organic fluids and high expansion ratio required in an ORC turbine leads to highly supersonic flows in the turbine. Such high speed flows within a turbine stage are susceptible to high shock losses, if necessary corrective steps are not taken during early design phase. This has sparked a wave of interest in the scientific community to come up with an efficient supersonic ORC turbine design guidelines. The design of a supersonic turbine rotor is crucial to ensure efficient performance of the turbine. In the mid-20th century, a supersonic impulse rotor design methodology for axial turbines was proposed that boasts shock-free turning of supersonic flows using the vortex-flow theory. This procedure relies on Method of Characteristics (MOC) to solve hyperbolic governing PDEs. The method got enriched later with the capability to account for dense gas effects on the rotor geometry which is essential for ORC applications. However, there are no such design guidelines available in the literature to generate a radial rotor blade especially for supersonic flows. The conventional 1D preliminary design methods, generally available for radial rotor blades, are not very reliable in supersonic flow regime. This calls for a novel design philosophy for supersonic radial rotors that accounts for supersonic flow phenomenon early in the design phase to ensure efficient turbine performance. The objective of this thesis is to put forth a design methodology for supersonic radial rotors that acknowledges the flow equations in the design procedure. The proposed design ideology is to extend the supersonic axial rotor design procedure by including the fictitious forces' effects in the governing equations to generate a radial rotor using MOC. These pseudo forces (Centrifugal & Coriolis) are experienced by the flow in the radial runner if seen from the rotor's rotating frame of reference. Furthermore, the dense gas effects are also included in the design using the properties from CoolProp library. A design tool is developed in PYTHON that implements the aforementioned ideology in the design procedure. This thesis takes the first step towards developing a fully functional design methodology for supersonic radial rotors. Therefore, a simple case of a constant force's influence on the rotor geometry is studied in this work and compared with the CFD simulations in both perfect & dense gas region. Future research will focus on understanding the complex behaviour of organic fluids in the presence of an external force and modify the design procedure accordingly.Mechanical Engineering | Energy and Process Technolog
Application of Advanced Technologies for CO2 Capture from Industrial Sources
No full textThe great majority of the research on CO2 capture worldwide is today devoted to the integration of new technologies in power plants, which are responsible for about 80% of the worldwide CO2 emission from large stationary sources. The remaining 20% are emitted from industrial sources, mainly cement production plants (∼7% of the total emission), refineries (∼6%) and iron and steel industry (∼5%). Despite their lower overall contribution, the CO2 concentration in flue gas and the average emission per source can be higher than in power plants. Therefore, application of CO2 capture processes on these sources can be more effective and can lead to competitive cost of the CO2 avoided with respect to power plants. Furthermore, industrial CO2 capture could be an important early-opportunity application, or a facilitate demonstration of capture technology at a relative small scale or in a side stream.This paper results from a collaborative activity carried out within the Joint Programme on Carbon Capture and Storage of the European Energy Research Alliance (EERA CCS-JP) and aims at investigating the potentiality of new CO2 technologies in the application on the major industrial emitters
The First X-Ray Polarization Observation of the Black Hole X-Ray Binary 4U 1630-47 in the Steep Power-law State
No full textFull list of the authors: Rodriguez Cavero, Nicole; Marra, Lorenzo; Krawczynski, Henric; Dovčiak, Michal; Bianchi, Stefano; Steiner, James F.; Svoboda, Jiri; Capitanio, Fiamma; Matt, Giorgio; Negro, Michela; Ingram, Adam; Veledina, Alexandra; Taverna, Roberto; Karas, Vladimir; Ursini, Francesco; Podgorný, Jakub; Ratheesh, Ajay; Suleimanov, Valery; Mikušincová, Romana; Zane, Silvia; Kaaret, Philip; Muleri, Fabio; Poutanen, Juri; Malacaria, Christian; Petrucci, Pierre-Olivier; Gau, Ephraim; Hu, Kun; Chun, Sohee; Agudo, Iván; Antonelli, Lucio A.; Bachetti, Matteo; Baldini, Luca; Baumgartner, Wayne H.; Bellazzini, Ronaldo; Bongiorno, Stephen D.; Bonino, Raffaella; Brez, Alessandro; Bucciantini, Niccolò; Castellano, Simone; Cavazzuti, Elisabetta; Chen, Chien-Ting; Ciprini, Stefano; Costa, Enrico; De Rosa, Alessandra; Del Monte, Ettore; Di Gesu, Laura; Di Lalla, Niccolò; Di Marco, Alessandro; Donnarumma, Immacolata; Doroshenko, Victor; Ehlert, Steven R.; Enoto, Teruaki; Evangelista, Yuri; Fabiani, Sergio; Ferrazzoli, Riccardo; García, Javier A.; Gunji, Shuichi; Hayashida, Kiyoshi; Heyl, Jeremy; Iwakiri, Wataru; Jorstad, Svetlana G.; Kislat, Fabian; Kitaguchi, Takao; Kolodziejczak, Jeffery J.; La Monaca, Fabio; Latronico, Luca; Liodakis, Ioannis; Maldera, Simone; Manfreda, Alberto; Marin, Frédéric; Marinucci, Andrea; Marscher, Alan P.; Marshall, Herman L.; Massaro, Francesco; Mitsuishi, Ikuyuki; Mizuno, Tsunefumi; Ng, Chi-Yung; O'Dell, Stephen L.; Omodei, Nicola; Oppedisano, Chiara; Papitto, Alessandro; Pavlov, George G.; Peirson, Abel L.; Perri, Matteo; Pesce-Rollins, Melissa; Pilia, Maura; Possenti, Andrea; Puccetti, Simonetta; Ramsey, Brian D.; Rankin, John; Roberts, Oliver J.; Romani, Roger W.; Sgrò, Carmelo; Slane, Patrick; Spandre, Gloria; Soffitta, Paolo; Swartz, Douglas A.; Tamagawa, Toru; Tavecchio, Fabrizio; Tawara, Yuzuru; Tennant, Allyn F.; Thomas, Nicholas E.; Tombesi, Francesco; Trois, Alessio; Tsygankov, Sergey S.; Turolla, Roberto; Vink, Jacco; Weisskopf, Martin C.; Wu, Kinwah; Xie, FeiThe Imaging X-ray Polarimetry Explorer (IXPE) observed the black hole X-ray binary 4U 1630-47 in the steep power-law (or very high) state. The observations reveal a linear polarization degree of the 2-8 keV X-rays of 6.8% ± 0.2% at a position angle of 21.°3 ± 0.°9 east of north (all errors at 1σ confidence level). Whereas the polarization degree increases with energy, the polarization angle stays constant within the accuracy of our measurements. We compare the polarization of the source in the steep power-law state with the previous IXPE measurement of the source in the high soft state. We find that, even though the source flux and spectral shape are significantly different between the high soft state and the steep power-law state, their polarization signatures are similar. Assuming that the polarization of both the thermal and power-law emission components are constant over time, we estimate the power-law component polarization to be 6.8%-7.0% and note that the polarization angle of the thermal and power-law components must be approximately aligned. We discuss the implications for the origin of the power-law component and the properties of the emitting plasma. © 2023. The Author(s). Published by the American Astronomical Society.The Imaging X-ray Polarimetry Explorer (IXPE) is a joint US and Italian mission. The US contribution is supported by the National Aeronautics and Space Administration (NASA) and led and managed by its Marshall Space Flight Center (MSFC), with industry partner Ball Aerospace (contract NNM15AA18C). The Italian contribution is supported by the Italian Space Agency (Agenzia Spaziale Italiana, ASI) through contract ASI-OHBI-2022-13-I.0, agreements ASI-INAF-2022-19-HH.0 and ASI-INFN-2017.13-H0, and its Space Science Data Center (SSDC) with agreements ASI-INAF-2022-14-HH.0 and ASI-INFN 2021-43-HH.0, and by the Istituto Nazionale di Astrofisica (INAF) and the Istituto Nazionale di Fisica Nucleare (INFN) in Italy. This research used data and software products or online services provided by the IXPE Team (MSFC, SSDC, INAF, and INFN) and distributed with additional software tools by the High-Energy Astrophysics Science Archive Research Center (HEASARC), at NASA Goddard Space Flight Center (GSFC). N.R.C. and H.K. acknowledge NASA support through the grants NNX16AC42G, 80NSSC20K0329, 80NSSC20K0540, NAS8-03060, 80NSSC21K1817, 80NSSC22K1291, and 80NSSC22K1883 as well as support from the McDonnell Center for the Space Sciences. M.D., J.S., V.K., and J.P. acknowledge the support from the Czech Science Foundation project GACR 21-06825X and the institutional support from the Astronomical Institute of the Czech Academy of Sciences RVO:67985815. A.I. acknowledges support from the Royal Society. M.N. acknowledges the support by NASA under award number 80GSFC21M0002. P.O.P. acknowledges financial support from the French High Energy Programme (PNHE) of the CNRS as well as from the French Space Agency (CNES)
Author Correction: Comprehensive analysis of chromothripsis in 2,658 human cancers using whole-genome sequencing
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