101 research outputs found
Antibacterial Activity of Verbascum antinori
No full textDulger, Basaran/0000-0002-3184-2652WOS: 000450536200022Objective: In current study, biological screening of the methanol extract obtained from Verbascum antinori Boiss. et Heldr. (Scrophulariaceae) was carry out on antibacterial effects. Methods: Antibacterial activity of the extract was evaluated for Staphylococcus aureus ATCC 6538P, Proteus vulgaris ATCC 8427, Klebsiella pneumoniae UC57, Micrococcus luteus CCM 169, Escherichia coli ATCC 11230, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 7064 and Pseudomonas aeruginosa ATCC 27853 by disk diffusion method. Results: The extract has potential effect against the Gram-positive bacteria, but no activity was observed against the Gram-negative bacteria used in the present study. Conclusions: On the basis of the mentioned results, V. antinori may be a potential source of antibacterial agent
Anti-Staphylococcal Activity of Verbascum thapsus L. against Methicillin-Resistant Staphylococcus aureus
No full textDulger, Basaran/0000-0002-3184-2652WOS: 000404817500010Objective: In this study, it was aimed to investigate the anti-Staphylococcal activity of ethanol extract obtained from leaves of Verbascum thapsus L. (Scrophulariaceae) plant. Methods: The anti-Staphylococcal activity of the extract of ethanol extracted from the leaves of Verbascum thapsus L. (Scrophulariaceae) was investigated against Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria by Agarwell diffusion method and microdilution method. Results: The ethanol extract showed antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) strains (MIC < 1024 mu g/mL). When compared with Methicillin and Gentamicin, the extract was more effective against MRSA strains. Conclusion: The use of Verbascum thapsus L. (Scrophulariaceae) as an anti-Staphylococcal agent has been found to be appropriate in vitro and should be performed in vivo
Evaluating antimicrobial and antioxidant capacity of endemic Phlomis russeliana from Turkey and its antiproliferative effect on Human Caco-2 Cell Lines
No full textDulger, Basaran/0000-0002-3184-2652WOS: 000493504800001PubMed: 31365649In this study, the antimicrobial, antioxidant and antitumor activity of ethanol extracts obtained from Phlomis russeliana (Sims.) Lag. ex Benth. (Lamiaceae) were evaluated. Disc diffusion and microdilution methods were used to test the extracts for antimicrobial activity against seven bacteria strains (Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 10538, Proteus vulgaris ATCC 6899, Salmonella typhimurium CCM 5445 and Pseudomonas aeruginosa ATCC 27853) and four yeast strains (Kluyveromyces fragilis ATCC 8608, Rhodotorula rubra ATCC 70403, Debaryomyces hansenii DSM 70238 and Candida albicans ATCC 10239). Notably, they were more effective against the yeast strains than the bacterial strains. Of the yeast cultures, D. hanseii was among the most susceptible, having an inhibition zone of 16.2 mm with minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of 64(128)mu g/ml, respectively. For cytotoxic determination, Caco-2 cells were cultured as per ATCC protocol, and were treated with log concentrations (5-80 mg/ml) of P. russeliana. The potency of cell growth inhibition for each extract was expressed as an IC50 value. Moreover, oxidant capacity was evaluated via TOC assay. This product induced antiproliferative activity of 31.33% at 40 mg/nil and 20.96% at 80 mg/ml, without toxic effects on cells, although the oxidant capacity was decreased to 27.06 +/- 0.7 nm in the 80 mg/nilapplied group compared to 47.9 +/- 1.8 mn in the untreated one. Advanced pharmacological studies are needed to further evaluate P. russeliana for distinctive features
Short communication- AN INVESTIGATION ON ANTIMICROBIAL ACTIVITY OF ENDEMIC ORIGANUM SOLYMICUM AND ORIGANUM BILGERI FROM TURKEY
No full textThe methanol extracts obtained from endemic Origanum solymicum
P. H. Davis and Origanum bilgeri P. H. Davis have been
investigated for their antimicrobial activity. Antimicrobial activity
was determined Escherichia coli ATCC 11230, Stapylococcus
aureus ATCC 6538P, Klebsiella pneumoniae UC57,
Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris
ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis
CCM 2067, Listeria monocytogenes ATCC 15313,
Micrococcus luteus CCM 169, Candida albicans ATCC
10231, Rhodotorula rubra DSM 70403 and Kluyveromyces fragilis
ATCC 8608 by disc diffusion method. The plant extracts
demonstrated antimicrobial effect against bacteria and the yeast
cultures used in this study at different levels
Spectroscopic studies, antimicrobial activities and crystal structures of N-(2-hydroxy-3-methoxybenzalidene) 1-aminonaphthalene
No full textDulger, Basaran/0000-0002-3184-2652; Dulger, Basaran/0000-0002-3184-2652; Unver, Huseyin/0000-0003-3968-4385Schiff base N-(2-hydroxy-3-methoxybenzalidene)1-aminonaphthalene has been synthesized from the reaction of 2-hydroxy-3-methoxybenzaldehyde with 1-aminonaphthalene. The compound were characterized by elemental analysis, FT-IR, H-1 NMR, C-13 NMR and UV-visible techniques. The UV-visible spectra of the Schiff base were studied in polar and nonpolar solvents in acidic and basic media. The structure of the compound has been examined cyrstallographically. There are two independent molecules in the asymmetric unit. It crystallizes in the monoclinic space group P2(l)/c, with unit cell parameters: a=14,602(2), b=5,800(1), c= 16,899(1) Angstrom, V= 1394.4(2) Angstrom(3) D-x=1.321 g cm(-3) and Z = 4. The crystal structure was solved by direct methods and refined by full-matrix least squares to a find R = 0.041 of for 1179 observed reflections. The title compound's antimicrobial activities also have been studied. The antimicrobial activities of the ligand has been screened in vitro against the organisms Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538, Klebsiella pneumoniae UC57, Micrococcus luteus La 2971, Proteus vulgaris ATCC 8427, Pseudomonas aeruginosa ATCC 27853, Mycobacterium smegmatis CCM 2067, Bacillus cereus ATCC 7064 and Listeria monocytogenes ATCC 15313, the yeast cultures Candida albicans ATCC 1023 1, Kluyveromyces fragilis NRRL 2415, Rhodotorula rubra DSM 70403, Debaryomyces hansenii DSM 70238 and Hanseniaspora guilliemodii DSM 3432. (C) 2004 Elsevier B.V. All rights reserved
Antibiotic Susceptibility of Motile Aeromonas spp. Isolated from Saricay Stream (Canakkale, Turkey)
No full textDulger, Basaran/0000-0002-3184-2652; Dulger, Basaran/0000-0002-3184-2652WOS: 000318748600029This study was carried out for the determination of the physico-chemical and microbiological quality and antibiogram profiles of the Aeromonas spp. isolated from Saricay stream in Canakkale - Turkey. The results indicated that water from this stream were of poor microbiological quality and unfit for human consumption. A total of 13 Aeromonas species were isolated from sixty (21.66%) water samples. This strains were identified as Aeromonas caviae (53.84%), A. hydrophila (23.07%), A. veronii by. sobria (23.07%). All aeromonads (100%) strains were resistant to erythromycin, amoxycillin, ampicillin (except one A.hydrophila strain), but susceptible to gentamicin.Canakkale Onsekiz Mart University, Canakkale, TurkeyCanakkale Onsekiz Mart University [2010-36]The authors thank to Research Fund of Canakkale Onsekiz Mart University, Canakkale, Turkey (Project number: 2010-36) for the financial support
İdrar yolu enfeksiyonlarından i?zole edilen mikroorganizmalar üzerine Ballota acetabulosa yapraklarının antimikrobiyal aktivitesi
No full textThe methanolic extracts obtained from the leaves of Ballota acetabulosa (L.) Bentham (Lamiaceae) were investigated for their antimicrobial activities against the pathogens causing complicated urine tract infections (Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis and Candida albicans) by disc diffusion method and microdilution method. Some antibacterial and antifungal antibiotics were used as a positive reference standard to determine the sensitivity of the test microorganisms. The extracts showed strong antimicrobial activity against Escherichia coli with inhibition zones of 18.6 mm, with MIC's and MBC's of 32(64) ng/mL. Also, the extracts exhibited moderate activity against the other test microorganisms. The results demonstrate that the methanolic extract of the leaves of Ballota acetabulosa has significant activity and suggest that it may be useful in the treatment of infections
ANTIBACTERIAL ACTIVITY OF ENDEMIC BALLOTA NIGRA SUBSP. ANATOLICA AGAINST SOME HUMAN EYE PATHOGENS FROM TURKEY
No full textABSTRACT Objective: The ethanol extracts obtained from the leaves of Ballota nigra subsp. anatolica P.H. Davis (Lamiaceae) were evaluated for their antibacterial activity against some human eye pathogens. Methods: Hospital isolates of Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus capitis, S. aureus, Staphylococcus epidermidis, Moraxella nonliquefaciens, and Propionibacterium acnes were obtained. Antibacterial activity was determined in the extracts using disc diffusion method. Some antibacterial antibiotics were used as a positive reference standard to determine the sensitivity of the isolates. Results: The extracts showed strong antibacterial activity against M. nonliquefaciens and P. acnes, with inhibition zones of 18.2 mm and 19.2 mm, respectively. The extracts also exhibited moderate activity against the other isolates. Conclusion: The results demonstrate that the leaf extract of the plant has significant activity and suggest that it may be useful in the treatment of eye infections. Keywords: Ballota nigra subsp. anatolica, Antibacterial activity, Eye pathogens
Antifungal Activity of Hypericum havvae Against Some Medical Candida Yeast and Cryptococcus Species
No full textPurpose: To investigate the antifungal activities.of the individual as well as the combined hydroalcohol leaf and root extracts of endemic Hypericum havvae A. Guner (Hyperiaceae).Methods: Each dry powdered plant material (20 g) was soaked in 150 ml of aqueous ethanol (50: 50 %v/v) until complete saturation of the plant material. After the extracts were filtered and evaporated, the antifungal activity of the extracts was tested against medical yeast, Candida (C. albicans ATCC 10231, C. tropicalis ATCC 13808, C. guilliermondii ATCC 6260) and Cryptococcus (C. neoformans ATCC 90112 and C. laurentii 34142) species by visual broth microdilution method. Minimum inhibitory concentration (MIC) of the extract was derived from the results. Ketoconazole was used as the reference standard. Results: MIC values ranged from 3.12 to 25.00 mg/ml. The extracts exhibited strong antifungal effect against the yeast cultures but the combination of the plant extracts (leaf + root) possessed stronger antifungal potency against Candida albicans and Cryptococcus laurentii, with the same MIC value of 1.56 mg/ml.Conclusion: Our findings support the use of Hypericum havvae in traditional medicine for the treatment of fungal infections, especially Candidiasis.Keywords: Antifungal activity, Candida, Hypericum havvae, Candidiasi
Short communication- AN INVESTIGATION ON ANTIMICROBIAL ACTIVITY OF ENDEMIC ORIGANUM SOLYMICUM AND ORIGANUM BILGERI FROM TURKEY
No full textThe methanol extracts obtained from endemic Origanum solymicum
P. H. Davis and Origanum bilgeri P. H. Davis have been
investigated for their antimicrobial activity. Antimicrobial activity
was determined Escherichia coli ATCC 11230, Stapylococcus
aureus ATCC 6538P, Klebsiella pneumoniae UC57,
Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris
ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis
CCM 2067, Listeria monocytogenes ATCC 15313,
Micrococcus luteus CCM 169, Candida albicans ATCC
10231, Rhodotorula rubra DSM 70403 and Kluyveromyces fragilis
ATCC 8608 by disc diffusion method. The plant extracts
demonstrated antimicrobial effect against bacteria and the yeast
cultures used in this study at different levels
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