13,001 research outputs found
Facile preparation of water soluble curcuminoids extracted from turmeric (Curcuma longa L.) powder by using steviol glucosides
No full textAbstractCurcuminoids from rhizomes of Curcuma longa possess various biological activities. However, low aqueous solubility and consequent poor bioavailability of curcuminoids are major limitations to their use. In this study, curcuminoids extracted from turmeric powder using stevioside (Ste), rebaudioside A (RebA), or steviol glucosides (SG) were solubilized in water. The optimum extraction condition by Ste, RebA, or SG resulted in 11.3, 9.7, or 6.7mg/ml water soluble curcuminoids. Curcuminoids solubilized in water showed 80% stability at pH from 6.0 to 10.0 after 1week of storage at 25°C. The particle sizes of curcuminoids prepared with Ste, RebA, and SG were 110.8, 95.7, and 32.7nm, respectively. The water soluble turmeric extracts prepared with Ste, RebA, and SG showed the 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of 127.6, 105.4, and 109.8μg/ml, and the inhibition activities (IC50) against NS2B-NS3pro from dengue virus type IV of 14.1, 24.0 and 15.3μg/ml, respectively
Characterization of quinoa (Chenopodium quinoa) fermented by Rhizopus oligosporus and its bioactive properties
Get PDFAbstract Quinoa is a pseudocereal that contains high quality protein, minerals, vitamins, polyphenols, and phytosterols. In this study, quinoa was fermented by Rhizopus oligosporus (R. oligosporus) up to 5 days and the functional compounds (l-carnitine, GABA, vanillic acid and gallic acid) were analyzed by LC/MS. The amounts of l-carnitine and GABA were 0.13 mg/kg and 540 mg/kg for nonfermented quinoa (NF), 3.15 mg/kg and 1040 mg/kg for fermented quinoa at 3 days (3F), and 1.54 mg/kg and 810 mg/kg for fermented quinoa at 5 days (5F). The vanillic acid and gallic acid were 1.3 and 0.1 mg/kg for NF, 1.55 and 2.37 mg/kg for 3F, and 1.83 and 0.84 mg/kg for 5F, respectively. Total phenolic contents and total flavonoids contents were 41 mg gallic acid (GAE)/kg and 13 mg quercetin equivalent (QE)/kg for NF, 74 mg GAE/kg and 16 mg QE/kg for 3F, and 80 mg GAE/kg and 19 mg QE/kg for 5F, respectively. Antioxidant activity (SC50) was 3.6 mg/mL for NF, 3.4 mg/mL for 3F, and 2.3 mg/mL for 5F. Nitric oxide production on RAW264.7 macrophages of fermented quinoa revealed 29% and 56% inhibition of nitric oxide production for NF and 5F, respectively. Therefore, fermented quinoa can be used as a healthy and valuable food product
Decrease of insoluble glucan formation in Streptococcus mutans by co-cultivation with Enterococcus faecium T7 and glucanase addition
No full textTo develop preventive canine oral health bio-materials consisting of probiotics and glucanase to reduce insoluble glucan and volatile sulfur compound formation. Co-cultivation of Enterococcus faecium T7 with Streptococcus mutans at inoculation ratio of 3:1 (v/v) resulted in 25% reduction in the growth of Streptococcus mutans. Amounts of soluble and insoluble glucans produced by S. mutans were decreased to 70 and 55%, respectively. Insoluble glucan was decreased from 0.6 A mu g/ml in S. mutans culture to 0.03 A mu g/ml in S. mutans co-cultivated with E. faecium T7 in the presence of Lipomyces starkeyi glucanase. Volatile sulfur compound, a main component of halitosis produced by Fusobacteria nucleatum, was decreased by co-cultivating F. nucleatum with E. faecium. E. faecium and glucanase can be combined as potentially active ingredients of oral care products for pets by reducing plaque-forming bacteria growth and their by-products that cause cavity and periodontal disease.OAIID:RECH_ACHV_DSTSH_NO:T201719155RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A079459CITE_RATE:1.846FILENAME:BL pet 2018 난수용 30 탄한30 식품독성20 한인도20.pdfDEPT_NM:국제농업기술학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/11a0e4c5-a73f-4837-8b46-5f131ef0210b/linkY
Synthesis and Functional Characterization of Caffeic Acid Glucoside Using <i>Leuconostoc mesenteroides</i> Dextransucrase
No full textCaffeic acid was modified via. transglucosylation using sucrose and dextransucrase-from Leuconostoc mesenteroides B-512FMCM. Following enzymatic modification, a caffeic acid glucoside was isolated by butanol separation, silica gel chromatography, and preparative HPLC. The synthesized caffeic acid glucoside had a molecular mass-to-charge ratio of 365 m/z, and its structure was identified as caffeic acid-3-O-alpha-D-glucopyranoside. The production of this caffeic acid-3-O-alpha-D-glucopyranoside at a concentration of 153 mM was optimized using 325 mM caffeic acid, 355 mM sucrose, and 650 mU mL(-1) dextransucrase in the synthesis reaction: In comparison with the caffeic acid, the caffeic acid-3-O-alpha-D-glucopyranoside displayed 3-fold higher water solubility, 1.66-fold higher antilipid peroxidation effect, 15% stronger inhibitiOn of colon cancer cell growth, and 11.5-fold higher browning resistance. These results indicate that this caffeic acid-3-O-alpha-D-glucopyranoside may be a suitable functional component of food and pharmaceutical products.OAIID:RECH_ACHV_DSTSH_NO:T201702232RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A079459CITE_RATE:3.154FILENAME:JAFC caffecic acid 2017.pdfDEPT_NM:국제농업기술학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/b959a5dc-011a-43a0-a98e-77e5602513bd/linkN
Author Correction: Evaluation of skin cancer resection guide using hyper‑realistic in‑vitro phantom fabricated by 3D printing
Get PDFThe original version of this Article contained an error in the spelling of the author Taehun Kim which was incorrectly given as Teahun Kim. The original Article has been corrected
‐carnitine fortified Makgeolli brewed by using fermented buckwheat
No full textMakgeolli is a traditional Korean alcoholic rice beverage. It is brewed of ingredients containing starch, Nuruk, and water. In order to improve the quality and functionality of Makgeolli, the Rhizopus oligosporus fermented buckwheat containing 18.7 mg/kg of l-carnitine were utilized to brew l-carnitine fortified Makgeolli with rice. Makgeolli was prepared in two-stage fermentation method and total rutin and quercetin in each fermented buckwheat Makgeolli were increased 1.8-fold greater than buckwheat Makgeolli. DPPH antioxidant activity was enhanced in fermented buckwheat Makgeolli than buckwheat Makgeolli (21.9%-65.7%). The amounts of l-carnitine in rice Makgeolli, buckwheat Makgeolli, and fermented buckwheat Makgeolli were 0.9, 0.8-1.0, and 1.0-1.9 mg/L, respectively. The fermented buckwheat Makgeolli not only promoted health benefit by increasing l-carnitine and flavonols, but also made effective alcohol production (2.8%-8.4%) compared to common buckwheat Makgeolli, indicating the potential industrial application with health benefits.OAIID:RECH_ACHV_DSTSH_NO:T201811172RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A079459CITE_RATE:1.521FILENAME:Park_et_al-2018-Food_Science_&_Nutrition.pdfDEPT_NM:국제농업기술학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/5227de8e-e24e-4969-80df-870a455999d4/linkY
Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B1299CB4
No full textEpigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. Each EGCG-G was purified using a C-18 column. Of nine EGCG-Gs identified by nuclear magnetic resonance analysis, five EGCG-Gs (2 and 4-7) were novel compounds with yields of 2.2-22.6%. The water solubility of the five novel compounds ranged from 229.7 to 1878.5 mM. The 5'-OH group of EGCG-Gs expressed higher antioxidant activities than the 4'-OH group of EGCG-Gs. Furthermore, glucosylation at 7-OH group of EGCG-Gs was found to be responsible for maintaining tyrosinase inhibitory activity and increasing browning-resistant activities.N
Biological characterization of epigallocatechin gallate complex with different steviol glucosides
No full textSteviol glucosides (SGs) such as rubusoside (Ru), stevioside (Ste), rebaudioside A (RebA) and stevioside glucosides (SG) are herbal tea sweeteners that enhance the solubility and stability of a number of pharmaceutically important compounds. The complex of epigallocatechin gallate (EGCG) with 10% (w/v) each Ru, Ste, RebA or SG enhanced the water solubility of EGCG over 15 times to 345, 312, 341, or 320 mg/mL, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of EGCG, EGCG-Ru, EGCG-Ste, EGCG-RebA, and EGCG-SG in water were 5.88, 6.03, 6.52, 4.89, and 4.23 mu g/mL, respectively. EGCGs complexed with different SGs maintained inhibitory activities against human intestinal maltase, human pancreatic alpha-amylase, and the growth of Streptococcus mutans, Helicobacter pylori, Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Clostridium difficile. In glucose tolerance test using C57BL/6 mice, plasma glucose levels in mice treated with EGCG or EGCG-Ste complex were decreased by 9.34%, which was 31.08% lower than those treated with maltose. The efficient and cost-effective EGCG-SGs production method might be applicable to produce water soluble bioactive nutraceuticals in large scale.OAIID:RECH_ACHV_DSTSH_NO:T201718366RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A079459CITE_RATE:1.226FILENAME:BBE EGCG-G 2017.pdfDEPT_NM:국제농업기술학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/b1f28b31-16b8-476c-84f9-dcdad7c4a4a2/linkN
Neem seed oil and gum arabic-based oil-in-water emulsions as potential ocular drug delivery system
No full textThe present study deciphers the synthesis and characterization of the neem seed oil (NSO) and gum arabic (GA) based emulsion. The synthesized emulsions were thoroughly characterized for probable application in ocular drug delivery. The synthesized emulsions were white in color. The FTIR spectroscopy divulged that the glycoprotein complex of GA retained its functional architecture. The formation of the oil-in-water emulsion was confirmed by confocal microscopy. The NSO droplets in the emulsions behaved as capacitive elements whose properties altered as the composition of the emulsions were varied. The mechanical studies suggested that the interactions within the emulsion components tailored the polymer-polymer integrity under stress. The drug release and corneal permeation studies using ciprofloxacin HCl-loaded emulsions indicated that the ciprofloxacin molecules were released from the emulsions, and the emulsions were able to deliver the drug across the corneal tissue. The prepared emulsions were nonirritant to the ocular tissues. The released drug was capable of inhibiting the growth of the model bacteria (Staphylococcus aureus). In gist, the prepared emulsions were suitable for ocular drug delivery applications. [GRAPHICS] .N
Production of steviol from steviol glucosides using β-glucosidase from a commercial pectinase, Sumizyme PX
No full textTo purify and characterize a specific enzyme from a commercial pectinase for the production of steviol from stevioside (Ste) without adding organic solvent and to improve steviol production. Commercial Sumizyme PX converted Ste to steviol with a yield of 98%. beta-Glucosidase from Sumizyme PX (beta glyPX) was purified in three steps with 12.5-fold purification and 51% yield. The specific activity of the purified beta glyPX was 141 U/mg. The molecular weight of beta glyPX was similar to 116 kDa on SDS-PAGE. Its optimum activity was at pH 3.5 and 65 A degrees C. It was stable for 12 h up to 55 A degrees C and for 24 h at pH 2-9.5. K (m) values of beta glyPX for pNPGal, oNPGlc, lactose, and Ste were 2.4, 0.7, 18, and 7.8 mM, respectively. The optimum conditions for steviol production were 55 A degrees C, 900 U/ml, 80 mg Ste/ml, 12 h. beta glyPX contains great potential for industrial steviol production from Ste.OAIID:RECH_ACHV_DSTSH_NO:T201719167RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A079459CITE_RATE:1.846FILENAME:Production of steviol from steviol gluc식품독성 20 고부가가30 난수용 10 한일10 한인도 10탄한20.pdfDEPT_NM:국제농업기술학과EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/e433f73e-d049-45e2-a9fd-8934e432f09b/linkN
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