12 research outputs found
(Trematoda: Plagiorchiida: Opisthorchioidea): an unexpected systematic position
No full textFigure 5. Phylogenetic position of Liliatrema based on dataset of 18S+28S rRNA genes sequences alignment. A, reconstruction with maximum likelihood algorithm using Bayesian nodal support; B, fragment of tree with topology of the Opisthorchiidae in Bayesian algorithm. The posterior probabilities less than 0.9 are not indicated. References for data retrieved from GenBank are listed in Table 1.Published as part of Sokolov, Sergey, Frolov, Evgeniy, Novokreshchennykh, Semen & Atopkin, Dmitry, 2021, Zoological Journal of the Linnean Society 192 (1) on page 24, DOI: 10.1093/zoolinnean/zlaa093, http://zenodo.org/record/530100
Liliatrema Gubanov 1953
No full textGENUS <i>LILIATREMA</i> GUBANOV, 1953 <p> <i>Diagnosis:</i> With characters of family. Type species <i>L. skrjabini</i> Gubanov, 1953.</p> <p>The four subfamilies differ from the 12 (without the Pachytrematinae) earlier established opisthorchiid subfamilies (see: Scholz, 2008) most clearly in the following features: the Liliatrematinae, by the presence of a specialized oral sucker, the uroproct and the genital sac without gonotyl; the Apophalinae, by the presence of a median permanent ventrogenital sac with a welldeveloped ventral sucker and two gonotyls, which arise dorsally close against the ventral sucker overhanging it ventrally; the Cryptocotylinae, by the presence of a median permanent ventrogenital sac with an anterior pocket and a rudimentary ventral sucker located on the large protractile bulge, but without gonotyl; the Euryhelminthinae, by the presence of a median permanent ventrogenital sac with a well-developed ventral sucker and an elliptical or reniform, largely parenchymatose gonotyl. In this updated concept, the family Opisthorchiidae has lost its former essential difference with respect to Heterophyidae, which was the presence of the genital or the ventrogenital sac [compare with Bray (2008)]. The opistorchiids with an unspecialized (in particular, unarmed) oral sucker and the median genital or ventrogenital sac – apophallines, cryptocotylines and euryhelminthines – differ from the heterophyids with the same features in the morphology of the ventral sucker or gonotyl (if any). The liliatrematines possess a combination of features, which does not occur among other opisthorchioids, namely, the presence of a uroproct and a funnelshaped oral sucker with a penta- or heptagonal distal end, whose dorsal edge is covered with a hood-like tegumental fold. At the same time, the differences between the Opisthorchiidae and the Cryptogonimidae, previously identified by Bray (2008), are still valid.</p> <p> The phylogenetically based taxonomy of the Opisthorchioidea is still being developed. The current taxonomic model of this superfamily (see: Bray, 2008) is imperfect because of the polyphyly of the family Heterophyidae, which was revealed repeatedly by the nuclear DNA sequence analyses (Olson <i>et al.</i>, 2003; Sato <i>et al.</i>, 2010; Thaenkham <i>et al</i>., 2011, 2012; Fraija- Fernández <i>et al.</i>, 2015; Le <i>et al.</i>, 2017; Kuzmina <i>et al.</i>, 2018; Pantoja <i>et al.</i>, 2018; Hernández-Orts <i>et al.</i>, 2019; Kohl <i>et al.</i>, 2019; Tatonova & Besprozvannykh, 2019; this study). The present study and that of Tatonova & Besprozvannykh (2019) are the first attempts to resolve this problem. However, a discussion of the concrete steps towards a further revision of the Heterophyidae is currently impossible, because out of the several early-branching groups of these trematodes, only one is statistically supported in different approaches of our phylogenetic analysis (Figs 4A, 5A).</p> <p> The absence of molecular data for 10 (without the Pachytrematinae) opisthorchiid subfamilies (see: Scholz 2008), a labile position of <i>Liliatrema</i>, <i>Apophallus</i> and <i>Euryhelmis</i> on 28S rDNA and 18S+28S rDNA-based trees, indicate that the proposed concept of the Opisthorchiidae is preliminary. In future, the subfamilies Apophallinae, Cryptocotylidae Euryhelminthinae and Liliatrematidae might yet be dissolved or reduced to the status of a tribe or subtribe. The current phylogenetic data and previously published materials (e.g. Thaenkham <i>et al.</i>, 2012; Tatonova <i>et al.</i>, 2020) cast doubt on the monophyly of the subfamily Opisthorchiinae. Thus, this subfamily needs revision.</p>Published as part of <i>Sokolov, Sergey, Frolov, Evgeniy, Novokreshchennykh, Semen & Atopkin, Dmitry, 2021, An opisthorchiid concept of the genus Liliatrema (Trematoda: Plagiorchiida: Opisthorchioidea): an unexpected systematic position, pp. 24 in Zoological Journal of the Linnean Society 192 (1)</i> on page 24, DOI: 10.1093/zoolinnean/zlaa093, <a href="http://zenodo.org/record/5301001">http://zenodo.org/record/5301001</a>
Genetic differentiation of black and grey colored forms of the earthworm Drawida ghilarovi Gates, 1969 (Moniligastridae, Oligochaeta) on Russian Far East
No full textMolecular differentiation of epigeic and anceic forms of Drawida ghilarovi Gates, 1969 (Moniligastridae, Clitellata) in the Russian Far East: Sequence data of two mitochondrial genes
No full textPhylogenetic assessment of Apophallines (Digenea: Opisthorchiidae) with revision of Apophallus donicus Skrjabin & Lindtrop, 1919 complex and some taxonomic propositions
No full textAccording to the current taxonomic concept, the Аpophallinae Ciurea, Citation1924 is a monotypic subfamily of the Opisthorchiidae that comprises species with a median permanent ventrogenital sac containing a well-developed ventral sucker and two gonotyls. A recently formulated molecular hypothesis suggests polyphyly of this subfamily and the presence of two sibling species within one of the nominal species A. donicus Skrjabin & Lindrop, Citation1919. In this paper, we redescribed A. donicus s. str. and renamed A. donicus auct. non Skrjabin & Lindrop, Citation1919 to A. lari (Leonov, Citation1957) based on morphological, biological and molecular (mitochondrial cox1 gene fragments) data. The main morphological differences between species are the length of the distance between the anterior end of the body and the anterior extremity of the vitellarium as a ratio to the forebody length, and the shape of the anterior vitelline follicles. We also aimed to test the monophyly of the Аpophallinae through nuclear-ribosomal molecular markers 18S + 28S rDNA. Tree topology showed Apophallus zalophi (Price, Citation1932) clustered closer to Liliatrema Gubanov, 1953 than to the other Apophallus spp. As a result, we resurrect the genus Pricetrema Ciurea, Citation1933 that earlier was proposed for A. zalophi and abolish the Liliatrematinae Gubanov, 1953 with removal of Liliatrema into the Apophallinae. Thus, according to the updated concept, the Apophallinae contains three genera, Apophallus Lühe, 1909, Liliatrema and Pricetrema, two of which (Apophallus, Pricetrema) have the ventrogenital sac with two gonotyls and one (Liliatrema) has a genital sac without gonotyls
The life cycle of Asymphylodora perccotti sp. n. (Trematoda: Lissorchiidae) in the Russian Southern Far East
No full textComparative phylogeography of four Apodemus species (Mammalia: Rodentia) in the Asian Far East: evidence of Quaternary climatic changes in their genetic structure
No full textCorresponding author. E-mail: [email protected] audienceThe phylogeography of four Apodemus species (Apodemus agrarius, Apodemus peninsulae, Apodemus latronum, and Apodemus draco) was studied in the Far East of Asia, based on sequences of the mitochondrial DNA cytochrome b gene. The results obtained show the existence of many different genetic lineages within the studied Apodemus species, suggesting the isolation and differentiation of populations in multiple refuge areas. Higher genetic diversities in some regions such as Yunnan, Sichuan (China), and eastern Russia suggest these areas are potential refuges for these species. The existence of such complex genetic structures could be linked to the presence of many biogeographic barriers (Himalaya Mountains, Tien-shan Mountains, Altai Mountains, Tibetan Plateau, Gobi desert, Yunnan Guizhou Plateau, Dzungaria basin, and others) in these regions, which were probably reinforced during the Quaternary climate changes. These barriers also played an important role concerning the low dispersal abilities of the two studied Apodemus species adapted to forest habitats (A. latronum and A. draco) with respect to colonizing regions other than Chin
Phylogenetic position of the genus Gonocerca Manter, 1925 (Trematoda, Hemiuroidea), based on partial sequences of 28S rRNA gene and a reconsideration of taxonomic status of Gonocercinae Skrjabin et Guschanskaja, 1955
No full textThe Identification of Araliaceae Species by ITS2 Genetic Barcoding and Pollen Morphology
No full textAbstractThe genetic barcode ITS2 (ITS: internal transcribed spacer) and pollen morphology were used for the identification of the pharmacologically valuable wild Araliaceae species Panax ginseng, Oplopanax elatus, Aralia elata, Aralia continentalis, Eleutherococcus senticosus, and Eleutherococcus sessiliflorus inhabiting the natural forests of Primorye, Russia. The ITS2 locus successfully identified all six species, which supports the use of ITS2 as a standard barcode for medicinal plants. However, the ITS2 locus was insufficient for intra-specific discrimination in these species, neither within Primorye nor from other world representatives within GenBank. Araliaceae pollen was confirmed to undergo size-reducing metamorphosis. The final morphotypes were species-specific for each of the six species but could not discriminate intra-species geographic localities within Primorye. The morphologies of the final pollen morphotypes from homologous species inhabiting other parts of the world are not yet known. Therefore, whether pollen is applicable for Araliaceae intra-species discrimination between Primorye and other world localities could not be established. Based on these findings, we propose that the ITS2 genetic barcode and the final pollen morphotypes are suitable for the identification of Araliaceae species. However, further studies will be needed to determine the suitability of genetic and pollen traits for Araliaceae geographic authentication.</jats:p
Comparative phylogeography of four Apodemus species (Mammalia: Rodentia) in the Asian Far East: evidence of Quaternary climatic changes in their genetic structure
No full textpeer reviewedThe phylogeography of four Apodemus species (Apodemus agrarius, Apodemus peninsulae, Apodemus latronum, and Apodemus draco) was studied in the Far East of Asia, based on sequences of the mitochondrial DNA cytochrome b gene. The results obtained show the existence of many different genetic lineages within the studied Apodemus species, suggesting the isolation and differentiation of populations in multiple refuge areas. Higher genetic diversities in some regions such as Yunnan, Sichuan (China), and eastern Russia suggest these areas are potential refuges for these species. The existence of such complex genetic structures could be linked to the presence of many biogeographic barriers (Himalaya Mountains, Tien-shan Mountains, Altai Mountains, Tibetan Plateau, Gobi desert, Yunnan Guizhou Plateau, Dzungaria basin, and others) in these regions, which were probably reinforced during the Quaternary climate changes. These barriers also played an important role concerning the low dispersal abilities of the two studied Apodemus species adapted to forest habitats (A. latronum and A. draco) with respect to colonizing regions other than China. © 2010 The Linnean Society of London
