269 research outputs found
The global food chain
No full textJonathon Porritt is Founder Director of Forum for the Future www.forumforthefuture.org.uk; Chairman of the UK Sustainable Development Commission www.sd-commission.org.uk; and author of Capitalism as if the World Matters (Earthscan, 2007)
Solubility Product Determines Two-Component Phase Separation of Multivalent Model Proteins
No full textBiomolecular condensates function in cellular organization and signal transduction by concentrating specific biomolecules. Condensates form through multivalent interactions between proteins and can contain hundreds of biomolecules. Condensates formed via homotypic interactions phase separate above a critical concentration (csat). However, csat is inadequate to describe complex heterotypic condensates, cellular condensates, therefore understanding phase behavior of these condensates becomes challenging. Previously, our collaborators used spatial simulations to predict that the maximum solubility product defines condensate formation of multi-components systems. Here, I use biochemical phase separation assays with two multivalent proteins to demonstrate that the solubility product peaks when condensates are formed. Addition of heterotypic components to the mixtures targets more biomolecules to condensates. Therefore, the solubility product and concentration outside condensates decrease after the maximum is reached. These findings establish a foundation for defining condensate formation and can be applied to other complex in vitro and cellular systems with different interaction types.M.Sc.2025-11-13 00:00:0
Interrogating Linker-Mediated Intramolecular Interactions in PSD-95
No full textPostsynaptic density protein 95 (PSD-95) is a scaffold protein that organises ionotropic glutamate receptors in the postsynaptic density (PSD) to facilitate excitatory neuronal signalling and synaptic plasticity. PSD-95 interacts with binding partners through specific domain-motif interactions to form phase separated clusters via multivalent interactions. While PSD-95 domains have been extensively studied, the contributions of its intrinsically disordered regions (IDR) to protein interactions and phase separation remain undefined. IDRs – also termed linkers – can regulate spatial orientations of adjacent structured domains or participate in noncanonical binding interactions; phosphorylation of linkers in these systems modifies their conformations and consequently phase separation behaviour. We report differential contributions of PSD-95 regions to its self-association and demonstrate that linkers – and their phosphorylation – may modulate intramolecular interactions. Our findings provide insight into the role of PSD-95 linkers in facilitating protein interactions and their potential to contribute to organisation of receptors in the PSD.M.Sc
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Destination-Based Brand Equity: The Impact of Destination Image on the destination stakeholders
No full textDr Jonathon Day
Dr Jonathon Day is an Associate Professor in Purdue’s School of Hospitality and Tourism Management. In addition to over 45 academic articles and chapters, he is the author of “Introduction to Sustainable Tourism and Responsible Travel” and co-author of “The Tourism System 8th Edition” . Dr Day’s research interests focus on sustainable tourism and responsible travel.
Gurpreet Kour
Gurpreet is an assistant professor at the Indian Institute of Management, Shillong. Her research interests include destination barnding and destination governance. Destination image has been an important topic in tourism research. The appeal of a destination’s image can contribute to a destination’s competitiveness. A wide range of factors contribute to the strength of the destination image, and the value extends beyond consumers to destination stakeholders. While a range of issues around destination image have been examined, there remains much to be explored. There is a paucity of research on the value created for these stakeholders by the brand image. There is also a gap in our understanding of the impact of destination image on destination stakeholder behavior. It is anticipated that operators in destinations with an image that emphasizes nature, and the environment will be more likely to undertake environmentally sustainable activities. This study aims to understand how the stakeholder perceives the value of the destination image and the impact of image considerations on stakeholders' actions in a destination
A conceptual review of interprofessional expertise in child safeguarding
No full textIt is increasingly accepted that practitioners across a range of professional fields must work together in order to promote children's welfare and protect them from harm. However, it has also become apparent that interprofessional working is a challenging area of practice that cannot simply be prescribed through protocols and procedures, nor acquired as a set of technical competences. This article develops the concept of interprofessional expertise in order to explain how practitioners become more proficient at working with others to manage complex child welfare issues. Key principles are outlined with reference to relevant theoretical frameworks, including models of skill acquisition. The article concludes by discussing some potential implications for future research and contemporary developments in child safeguarding practice
Allylic C—H activation to access anti-1,3-amino alcohol motifs
No full text1,3-Amino alcohols are common motifs in a variety of biologically active molecules including antivirals, antibiotics, antifungals, and various alkaloids. Due to their prevalence and utility as synthetic intermediates, a variety of methods have been developed to access these motifs in a diastereoselective fashion, which are outlined in detail herein.
This thesis documents a novel approach to access anti-1,3-amino alcohols through an intramolecular palladium (II)/sulfoxide-oxazoline catalyzed C—H functionalization between a terminal olefin and an N-tosyl carbamate, generating anti-1,3-oxazinanones. These motifs can be further elaborated upon, making this method ideal for the late stage diversification of complex molecules and pharmaceuticals. This new method can be carried out in the presence of reactive functionality that is not well tolerated by existing methods.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01The student, Jonathon Young, accepted the attached license on 2018-04-25 at 09:29.The student, Jonathon Young, submitted this Thesis for approval on 2018-04-25 at 09:40.This Thesis was approved for publication on 2018-04-25 at 14:08.DSpace SAF Submission Ingestion Package generated from Vireo submission #12470 on 2018-08-31 at 17:30:26Made available in DSpace on 2018-09-04T20:47:30Z (GMT). No. of bitstreams: 2
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Cholesterol and Calmodulin Drive CFTR Clustering on Model Membranes
No full textThe Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a chloride channel whose dysfunction leads to intracellular accumulation of chloride ions, dehydration of cell surfaces, and subsequent damage to airway and ductal organs. Beyond its function as a chloride channel, CFTR is a key molecular hub and regulator of ion homeostasis. CFTR forms clusters in live cells that are cholesterol-dependent, calcium-sensitive, abolished by the most common CF-causing mutation, F508del, and rescued by folding correctors. However, the biophysical mechanisms that govern CFTR clustering remain unknown. Elsewhere, membrane protein clustering has been reported to be essential for receptor function and downstream signaling, and furthermore to be mediated by protein and lipid phase separation. Here, we report the first successful reconstitution of CFTR clusters in vitro. We demonstrate that calmodulin and cholesterol are essential for CFTR clustering on model membranes. Our findings reveal a novel mechanism for CFTR regulation via phase separation, providing insights into how multiple inputs may affect its function as a channel and in ion homeostasis.M.Sc.2025-11-13 00:00:0
Data and script for Van Berkel et al: Can starlings use a reliable cue of future food deprivation to adaptively modify foraging and fat reserves?
No full textSupporting materials for:
Can starlings use a reliable cue of future food deprivation to adaptively modify foraging and fat reserves?
Menno van Berkela, Melissa Batesona, Daniel Nettlea and Jonathon Dunna*
aCentre for Behaviour and Evolution & Institute of Neuroscience, Newcastle University, Newcastle, UK
*Author for correspondence (email: [email protected]; telephone: (+44)7730015855; postal address: Institute of Neuroscience, Henry Wellcome Building, The Medical School, Framlington Place, Newcastle University, Newcastle upon Tyne, UK, NE2 4HH).
R script and 3 .csv files.</p
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A Qualitative Study on the Perceptions of Soft Commitment Devices in Tourism
No full textAilin Fei is a second-year PhD student studying Hospitality and Tourism Management at Purdue University. Ailin earned her bachelors and masters from the University of Houston both in hospitality management. Her research interests are in sustainable tourism, specifically, responsible and sustainable travel behavior. Her recent papers investigate the effects of travel pledges and dives into behavioral economics and sustainable tourism.
Dr. Jonathon Day, an internationally recognized sustainable tourism expert and an Associate Professor in Purdue’s White Lodging – J.W. Marriott, Jr School of Hospitality and Tourism Management, has over 30 years of experience in tourism management. Jonathon is the coauthor of The Tourism System 8th edition, and author of “Introduction to Sustainable Tourism and Responsible Travel”, as well as over 35 peer-reviewed. He was recognized by the International Hospitality Institute as one of the Global 25 most influential educators in hospitality and the Global Top 30 Champions of Environmental Sustainability in Hospitality and Tourism for 2021.The negative impacts of mass tourism is attracting attention and tourism destinations are looking for ways to promote responsible travel behaviors among tourists. Commitment devices are being used to promote such behaviors. There are two types of commitment devices: soft and hard. studies have explored how soft commitment devices are being perceived by tourists. This study will explore the perceptions of three soft commitment devices being implemented in the tourist experience through the tourists’ perception. This study will provide key insights into the positive and/or negative attitudes toward the commitment devices being used to increase sustainable travel behaviors among tourists. Data will be collected through semi-structured interviews. The results of this study will reveal how tourists interpret the use of soft commitment devices and their perceived impact of such tools. This paper provides new insights about the practicalities of soft commitment devices used to promote sustainable travel behavior
Historical legacies, competition and dispersal control patterns of invasion by a non-native grass, Microstegium vimineum Trin. (A. Camus)
No full textBiological invasions can be controlled by a number of factors, and the importance of these factors for individual species can change depending on the spatial scale under consideration. Furthermore, the long-term legacies of past land use may be among the major factors structuring contemporary communities. This study was designed to merge these two lines of thought by observing the processes influencing invasion success of an exotic grass species, Microstegium vimineum. Plots within twenty hardwood forest stands of different ages (40 years old to [greater than] 100 years) were surveyed in central New Jersey for the presence and abundance of this species, and the data was analyzed using simple, multiple linear and logistic regressions, as well as regression trees. At the stand scale, M. vimineum abundance was influenced by age, slope, shrub cover and forest connectivity, with far more predictable responses in younger stands than in old. On the plot scale, forest age and prior use were again the dominant influences on abundance of the invasive. Invasion in old forests was most promoted by thinner canopy cover, while younger stands were most influenced by soil type and distance to forest edge. Further studies along dispersal corridors (streams and trails) in these stands consistently showed more invasion in younger stands than older, but also found the intensity of invasion to be higher along such corridors than through areas without them. Observation of isolated patches confirmed that seed dispersal is mostly short-range without dispersal conduits, with most seeds germinating less than a meter from their parent patch boundaries. Finally, an experimental study of the role of understory shrub cover and litter depth on seed germination and seedling growth showed that shade from shrubs strongly limits survival and growth. Seeds also need to be in contact with mineral soil to establish, but otherwise litter depth does not influence growth. These studies demonstrate that the establishment and spread of this invasive species is affected by complex hierarchies of factors that differ in stands with different land use histories. Land managers can use these differences to design targeted control programs that are more effective and stand-specific.Ph.D.Includes bibliographical references (p. 148-159)
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