118 research outputs found
Internationalizing-Innovation Profiles and High-Technology Exports: Does Lone Genius Matter?
No full textInnovation-performance research, when conducted at the firm level, neglects the role of innovation that is created without firm involvement. In this article, the authors test Schumpeter's lone-genius hypothesis: "Change in economic life always starts with the actions of a forceful individual." To do so, the authors introduce country-level internationalizing innovation profile (HP), which characterizes a country's innovation resources, and internationalizing-innovation experience (HE), which characterizes a country's level of patenting activity into the United States. Using fixed-effects panel data analysis for SO countries from 1990 through 2010, the authors demonstrate that a country's HP moderated by HE influences high-technology exports. The findings suggest that lone genius does have an impact, depending on the phase of a country's HE development. The implications of these findings for theory, public policy, and international marketing managers are discussed.Ewing Marion Kauffman Foundation; National Science Foundation; Kentucky Science and Technology Corporation (Kentucky Cabinet for Economic Development)[email protected]; [email protected]; [email protected]
Effect of egg yolk extract on proliferation and myogenic differentiation of C2C12 myoblasts
No full textMuscle wasting disorders like atrophy and sarcopenia are characterized by compromised skeletal muscle mass and function. One of the many causes of muscle wasting reported is the failure of satellite cells to fuse and form myofibres. Satellite cells are an integral part of skeletal muscle regeneration because of their self-renewal and differentiation abilities. Muscle regeneration research with respect to satellite cells has stimulated interest in discovering factors that enhance proliferation and differentiation as a first step in the regenerative process. The importance of nutrition in regulating muscle mass and muscle protein synthesis is well established. However, limited literature is available regarding the role of nutrition in muscle regeneration; specifically with respect to satellite cells. As for nutrition, chicken egg yolk appears to be a promising candidate. Egg yolks are rich in nutrients such as vitamins, minerals, amino acids and proteins which can provide sustained supply of nutrition to the cells in-vitro. In the present study, we tested the effect of different fractions and concentrations of egg yolk extract on C2C12 myoblasts. For this purpose, egg yolk extract was supplemented in growth media and cells were grown in this nutrient rich media for one week. Cell viability, fluorescence imaging and mRNA expression of muscle specific genes were analyzed at specific time points of proliferation and differentiation stages of myoblast. The study results showed that supplementation of the egg yolk extract in media enhanced proliferation and differentiation of myoblasts in a dose dependent manner which was analyzed by increase in cell viability, number of nuclei, number of myofibres and mRNA expression of muscle specific genes such as MyoD and myogenin.M.S.Includes bibliographical referencesby Devika Umesh Jogleka
Design of Infrared Roaster
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Estimation of Critical Micellar Concentration and Reverse Micelle Size.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Budhan Stories S1E2: Corona took many lives
No full textEpisode 2 of Season 1 is based on deaths happening in Chharanagar. During the first wave, Chharanagar people lost many lives due to Corona disease. The young Advocate Umesh Tamaychi was the first person who died in the civil hospital as during that time private hospitals were not allowed to treat Corona patients. After him so many others also died in Chharanagar. The episode focuses on those families who lost their near and dear ones.Directed (Author) by: Budhan Theatre Team. Participants: Dakxin Chhara, Atish Indrekar, Ruchika Kodekar, Chetna Rathod, Kushal Batunge, Keyur Bajrange, Anish Garange, Siddharth Garange, Umesh Tamaychi, Shefali Tamaychi, Dr Surya Bali, Bhopal, Akshay Khanna, Alice TilcheSupplementary materials include shortclips, poster and subtitles.</p
Reverse Micellar Extraction Coupled Adsorption for Purification of Bromelain
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Enzyme inactivation by using superheated steam.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Application of Dielectric Heating in Food Processing
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Acoustics Assisted Reverse Micellar Extraction Of Bromelain
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Selection and characterization of DNA aptamers for estradiol and ethynylestradiol for aptasensor development
No full textSmall organic contaminants have been widely detected in the surface and ground waters of this nation. A sub-class of these contaminants called endocrine disrupting compounds (EDCs) are known to have adverse effects on aquatic and human health. Among the EDCs, natural hormone 17β-estradiol (E2) and synthetic hormone 17α-ethynylestradiol (EE) possess high estrogenic potency and hence are contaminants of interest. Conventional methods to detect these compounds are expensive, time consuming and need implementation by an expert. By contrast, antibody-based assays are relatively inexpensive and commercially available but suffer from poor selectivity. A promising alternative makes use of DNA aptamers as molecular recognition elements. In order to evaluate the potential of DNA aptamers and aptasensors to detect small organics in natural waters, the following objectives were pursued: (1) critically review DNA aptamers and aptasensors developed for small organic molecules and assess their use for monitoring environmentally relevant organics, (2) select and characterize DNA aptamers that bind to E2 and EE and, (3) study the effect of immobilization on the binding affinity of the selected E2 and EE aptamers.
A review of ~80 aptamers and ~200 aptasensors for small organics was conducted to identify factors that affect binding affinity of the aptamer and limits of detection (LODs) of the aptasensor. Based on regression analyses, aptamer binding affinities are found to have a weak relationship with hydrophobicity of the target and length of the aptamer (p-values<0.05). Independent t-tests comparing aptasensor LODs suggest that the electrochemical platform is significantly more sensitive than colorimetric and fluorescence-based platforms. The inherent binding affinity of the aptamer was found to have a significant effect on the LOD of the aptasensor. While some fabricated aptasensors are sufficiently sensitive to detect contaminants at environmentally relevant concentrations, they are often associated with complex fabrication steps, and/or interference from structurally similar analogs. As a result, aptasensor commercialization faces many challenges including reusability, reproducibility and robustness.
In vitro selections were conducted with different selection pressures to isolate sensitive and selective DNA aptamers for E2 and EE. An equilibrium-filtration assay was used to determine dissociation constants (Kd) of the aptamer towards its parent target and its analogues. The E2 aptamers, E2Apt1 and E2Apt2 were found to have Kd values of 0.6 µM. They bound to analogue estrone (E1) with a similar affinity but were at least 74-fold more selective over EE. The EE aptamers Kd values are 0.5-1 µM. While one EE aptamer (EEApt1) was 53-fold more selective for EE over E2 and E1, the second EE aptamer (EEApt2) bound to all three EDCs (E1, E2 and EE) with similar affinities. The aptamers maintained their binding affinities in natural waters samples (tap water and lake water). DMS probing of the structure of the DNA aptamer revealed that the binding regions were mostly located in the single-stranded loop regions of the aptamer.
Aptasensors typically employ immobilized aptamers though the aptamers are selected and characterized while free or unattached in solution. The Kd values of immobilized selective aptamers were evaluated using magnetic microbeads surface for attachment. E2Apt1 immobilized at either end (5′ or 3′) and E2Apt2 immobilized at the 3′ end retain their binding affinity. The binding affinity is inversely correlated to the average linear distance of the binding pocket from the immobilized end. This result suggests that unwanted interactions between the aptamer and other moieties are more likely when the binding pocket is further away from the surface. Binding curve of E2Apt2 immobilized at the 5′ end indicates potential dimerization at high loadings of aptamer on the beads due to increased proximity between aptamer strands. EEApt1 loses its binding affinity upon immobilization potentially due to disruption in its tertiary structure upon attachment to the surface. Despite no loss in binding affinity upon immobilization, E2Apt1 (5′) shows no significant change in electrochemical current on binding to E2 when incorporated into an electrochemical sensor. This result implies an insufficient conformational change of the aptamer on binding to the target.
Overall, this work identifies the first aptamers for EE and selective aptamers for E2, while also highlighting the issues with development of aptamers and their eventual incorporation into aptasensors to detect small organics. Two major concerns are (1) immobilizing aptamers in sensor platforms while selections of aptamers are conducted with free/unattached aptamers, resulting in loss of binding affinity and (2) insufficient conformational change of the aptamer on binding to small molecule targets, resulting in a lack of change in the sensor signal. The findings from this dissertation support additional research directions regarding employing free aptamers in sensors and/or conducting new selections for aptamers using a DNA pool that is attached to a surface.Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01The student, Spurti Umesh Akki, accepted the attached license on 2018-07-09 at 14:53.The student, Spurti Umesh Akki, submitted this Dissertation for approval on 2018-07-09 at 15:05.This Dissertation was approved for publication on 2018-07-10 at 13:11.DSpace SAF Submission Ingestion Package generated from Vireo submission #12779 on 2018-09-27 at 11:18:33Made available in DSpace on 2018-09-27T16:30:28Z (GMT). No. of bitstreams: 3
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Previous issue date: 2018-07-10Embargo set by: Seth Robbins for item 107788
Lift date: 2020-09-27T16:30:34Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 107788
Lift date: 2020-09-27T16:31:43Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 107788
Lift date: 2020-09-27T16:34:29Z
Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD systemU of I Only Restriction Lifted for Item 107788 on 2020-09-28T09:15:16Z
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