12 research outputs found
DEGRADATION OF DIMETHYLTEREPHTHALATE BY NATURALLY FORMED MICROBIAL ASSOCIATIONS 169AC AND 189AC
No full textMixed microbial cultures, naturally combined in two-member associations
(169AC and 189AC), actively degraded the aromatic ester
dimethylterephthalate as a single source of carbon and energy. The
biodegrading activity of the associations and also of the pure culture
combination was tested under polysubstrate conditions. When
complementary substrates like methanol, xylol and ethylene glycol were
added, the consisting of a bacterial and an actinomyces strains
association 189, exhibited a higher activity. A biomass of this
association was brought to freeze-drying and the lyophilizate saved
biodegrading activity towards the studied substrate
DEGRADATION OF DIMETHYLTEREPHTHALATE BY NATURALLY FORMED MICROBIAL ASSOCIATIONS 169AC AND 189AC
No full textMixed microbial cultures, naturally combined in two-member associations
(169AC and 189AC), actively degraded the aromatic ester
dimethylterephthalate as a single source of carbon and energy. The
biodegrading activity of the associations and also of the pure culture
combination was tested under polysubstrate conditions. When
complementary substrates like methanol, xylol and ethylene glycol were
added, the consisting of a bacterial and an actinomyces strains
association 189, exhibited a higher activity. A biomass of this
association was brought to freeze-drying and the lyophilizate saved
biodegrading activity towards the studied substrate
DEGRADATION OF DIMETHYLTEREPHTHALATE BY NATURALLY FORMED MICROBIAL ASSOCIATIONS 169AC AND 189AC
No full textMixed microbial cultures, naturally combined in two-member associations
(169AC and 189AC), actively degraded the aromatic ester
dimethylterephthalate as a single source of carbon and energy. The
biodegrading activity of the associations and also of the pure culture
combination was tested under polysubstrate conditions. When
complementary substrates like methanol, xylol and ethylene glycol were
added, the consisting of a bacterial and an actinomyces strains
association 189, exhibited a higher activity. A biomass of this
association was brought to freeze-drying and the lyophilizate saved
biodegrading activity towards the studied substrate
Phenol Biodegradation by Two Xenobiotics-Tolerant Bacteria Immobilized in Polyethylene Oxide Cryogels
No full textBiofilms were formed on poly(ethylene oxide) (PEO) cryogels by using bacteria cultured from xenobiotics polluted environments and their phenol biodegrading capability was studied. PEO cryogels were synthesized via UV irradiation cross linking of moderately frozen aqueous system. Two xenobiotics tolerant bacterial isolates KCM R5 and KCM RG5 were used to construct the biofilms on the cryogels.
Obtained PEO-biofilms were assessed for their ability to remove phenol at concentrations 300, 400, 600 and 1000 mg L-1 for 28 days. The biofilm PEO-KCM RG5 removed phenol up to 600mg L-1/24 h, whereas the biofilm PEO-KCM R5 was able to degrade up to 1000 mg L-1/24 h. The high content of free-water in the cryogels allowed reproduction of the used bacteria. The high content of free-water in the cryogels allowed reproduction of the used bacteria. Short initial adaptation of the PEO-bio�lms with 100 mg L-1/24 h phenol was crucial for protecting the bacterial cells from dead. The obtained results showed that the liquid debit through the bio�lms on the 28th day of the experiments was lower than at the beginning. The cryogels demonstrated non-toxicity, high biocompatibility with bacteria and excellent mechanical characteristics. After aggressive phenol treatment the PEO-biofilms remained compact, porous and
elastic. The investigated new biological materials demonstrate potential for application in the industrial wastewater treatment technologies
MICROBIAL CULTURE ADAPTATION TO BIODEGRADATION OF DIMETHYLTEREPHTHALATE
No full textForty-five microbial cultures have been isolated from a chemically
polluted region, using dimethylterephthalate (DMT) as a sole carbon and
energy source. They have been subjected to adaptive selection in order
to increase their biodegradative potential. The gradual increase of the
xenobiotic substrate concentration from 2.5 to 25 mM induced adaptive
changes in the tested strains. 20% of the studied strains showed a high
biodegradative activity towards DMT and the best adaptation was
expressed by the soil microorganisms
MICROBIAL CULTURE ADAPTATION TO BIODEGRADATION OF DIMETHYLTEREPHTHALATE
No full textForty-five microbial cultures have been isolated from a chemically
polluted region, using dimethylterephthalate (DMT) as a sole carbon and
energy source. They have been subjected to adaptive selection in order
to increase their biodegradative potential. The gradual increase of the
xenobiotic substrate concentration from 2.5 to 25 mM induced adaptive
changes in the tested strains. 20% of the studied strains showed a high
biodegradative activity towards DMT and the best adaptation was
expressed by the soil microorganisms
MICROBIAL CULTURE ADAPTATION TO BIODEGRADATION OF DIMETHYLTEREPHTHALATE
No full textForty-five microbial cultures have been isolated from a chemically
polluted region, using dimethylterephthalate (DMT) as a sole carbon and
energy source. They have been subjected to adaptive selection in order
to increase their biodegradative potential. The gradual increase of the
xenobiotic substrate concentration from 2.5 to 25 mM induced adaptive
changes in the tested strains. 20% of the studied strains showed a high
biodegradative activity towards DMT and the best adaptation was
expressed by the soil microorganisms
Biodegradation of Phenol by Immobilized in Peo-Cryogel<i>Bacillus Laterosporus BT-271</i>in Sequencing Batch Biofilter
No full textEXTRA- AND INTRACELLULAR ESTERASES INVOLVED IN DIMETHYLTEREPHTHALATE CATABOLISM BY PSEUDOMONAS SP.
Get PDFThe strain Pseudomonas sp. 054, isolated previously from polluted soil,
possesses a high biodegrading activity towards the aromatic ester
dimethylterephthalate. The ester hydrolyzing activity of the strain was
investigated. Two types of esterases, extra- and intracellular, were
detected after growth in liquid medium. The enzyme activities were
proved in the culture supernatant and in the crude cell extract by the
use of a native electrophoresis. The extracellular protein with an
esterase activity had a molecular weight of 67 kDa, while the
intracellular fraction produced four separate bands with ester
hydrolyzing activity
Extra- and intracellular esterases involved in dimethylterephthalate catabolism by pseudomonas sp.
No full textThe strain Pseudomonas sp. 054, isolated previously from polluted soil,
possesses a high biodegrading activity towards the aromatic ester
dimethylterephthalate. The ester hydrolyzing activity of the strain was
investigated. Two types of esterases, extra- and intracellular, were
detected after growth in liquid medium. The enzyme activities were
proved in the culture supernatant and in the crude cell extract by the
use of a native electrophoresis. The extracellular protein with an
esterase activity had a molecular weight of 67 kDa, while the
intracellular fraction produced four separate bands with ester
hydrolyzing activity
