3 research outputs found
Identification of novel biomarkers for thyroid cancer using multi omics data analysis
No full textThe biomarkers for thyroid cancer are still not known properly. For treating thyroid cancer these biomarkers can by be targeted specifically. Through this project, we identified and used bioinformatics tools to find biomarkers associated with thyroid cancer. Gene Expression Omnibus database (GEO) was used to find dataset related with thyroid cancer. Their expression profiles were downloaded. Four dataset GSE3467, GSE3678, GSE33630, and GSE53157 were identified from GEO database. The dataset GSE3467 contains nine thyroid tumor samples and nine normal thyroid tissue samples. The GSE3678 contains seven thyroid tumor samples and seven normal thyroid tissue samples. The GSE53157 contains twenty four thyroid tumor samples and three normal thyroid samples. The GSE33630 contains sixty thyroid tumor samples and forty five normal thyroid samples. These four datasets were analyzed individually and were integrated at the end to find the common genes among these four datasets. The microarray analysis of the datasets were performed using excel. T.Test analysis were performed for all the four datasets individually on a separate excel sheet. The data was normalized by converting normal value into log scale. Differential expression analysis of all the four datasets were done to identify differentially expresses genes (DEGs). Only upregulated genes were taken into account. Principal component analysis (PCA) of all the four dataset were performed using the raw data. The PCA analysis were performed using T-BioInfo server and the scatterplots were prepared using excel. RStudio was used to match the gene symbols with the corresponding probe ids using left join function. Inner join function in R was used to find integrated genes between the four datasets. Heatmaps of all the four datasets were performed using RStudio. To find number of intersection of Differentially expressed genes, an upset plot was prepared using RStudio. 74 genes with their corresponding probe ids were found to be common among all the four datasets. These genes are common to at least two datasets. These 74 common genes were analyzed using Database for Annotation, Visualization, and Integrated Discovery (DAVID), to study their Gene onotology (GO) functional annotations and pathways. According to the GO functional annotations result, most of the integrated upregulated genes were involved in protein binding, plasma membrane and integral component of membrane. Most common pathway include Extracellular matrix organization, Neutrophil degranulation, TGF-beta signaling pathway and Epithelial to mesenchymal transition in colorectal cancer. These 74 genes were introduced to STRING database to find protein-protein interactions between the genes. Interactions between the nodes were downloaded from STRING database and introduced to Sytoscape. Sytoscape analysis explained that only 19 genes showed protein-protein interactions between each other. Disease free survival analysis of the 13 genes that were common to three datasets were done using GEPIA. Boxplots of these 13 genes were also prepared using GEPIA. This showed that these differentially expressed genes showed different expression in normal thyroid tissue and thyroid tumor samples. Hence these 13 genes common to 3 datasets can be used as potential biomarkers for thyroid cancer. Among these 13 genes, four genes are implicated in cancer/cell proliferation can be probable target for treatment options
Emergency poetics: postwar American poetry and the shape of public crisis
No full textFrom the formalization of Cold War civil defense to the rise of homeland security, emergency management in the United States has always been structured by power and privilege. “Emergency Poetics and the Shape of Public Crisis” tracks how postwar poets have responded to this trajectory by illuminating crisis conditions at the margins of state-sponsored emergency powers. Historically, the project follows the legacy of civil defense, a Cold War security paradigm that prioritized domestic comfort and security in the face of geopolitical threats. This insular paradigm later served as the basis for handling natural disasters, public health crises, and terrorism—all forms of public emergencies, declared or otherwise, that have magnified social and cultural exclusions in the name of national security. While recent studies of poetry have focused on discrete crisis genres such as terror, climate change, or financial collapse, this study reveals how multiple crisis genres are normalized and obscured, thus giving form to the lives and histories that have been rendered disposable along the way. I turn to a variety of poetic forms, from the shorter, witness-based poems of Denise Levertov and Essex Hemphill, to the longform multimodal experiments of Claudia Rankine and Cheena Marie Lo, to examine the way their experiments with address, temporality, citation, and constraint bring into view more historically attentive poetic modes of public care. By elevating forgotten bodies, affects, and temporalities outside the narrow frames of state-sponsored emergencies, these poets probe the limits of poetry’s powers to imagine how crises are rendered socially, politically, and culturally legible. Individual chapters focus on Denise Levertov’s “empathic projection” of wartime experience, from the homefront to the distant violence waged in Vietnam, Essex Hemphill’s elevation of the sensualized black gay body in protest of the federal government’s burying of the AIDS epidemic, Claudia Rankine’s catalogue of “lonely” subjects who are silenced by the racialized noise of the national security state, and Cheena Marie Lo’s attention to the many forms of institutional and cultural neglect that magnify the devastation of “natural” disasters. These poets have a shared investment in extending the way poetic subjects are made imaginatively available as witnesses to disaster, members of communities, citizens of a nation, and imaginers of a world that endures beyond them. This is perhaps the most important outcome of an emergency poetics: to be more alert to possibilities for non-hierarchical survival, hope, solidarity, and exchange even when they may not be available in the politics of the present. In this way, emergency poetics make the most dangerous contours of normalized violence visible in order to remake the terms of their exclusions—and, in turn, to remake the way we think about our capacity to care for others. Ph.D.Includes bibliographical reference
Bollywood Baddies Villains, Vamps and Henchmen in Hindi Cinema
No full textBollywood Baddies is the first-of-its-kind book-length narrative of villainy in Hindi films. It discusses villains, vamps, and henchmen of Bollywood cinema, and also the actors who essayed such characters over the decades. The author discusses not just villains but also the evaluation of villainous characters vis-à-vis sociopolitical conditions in the country. The narrative begins with Ashok Kumar's negative role in Kismet as early as 1943, and goes up to the Agneepath remake (2012), where Sanjay Dutt plays Kancha Cheena, earlier essayed by Danny Denzongpa in the original. In between, it discusses all major villains, from Lala Sukhiram (Mother India) to Gabbar (Sholay) to "Lion" Ajit (Kalicharan) to Mogambo (Mr. India), and many others. While keeping villains in the focus, it also discusses popular henchmen and vamps, like M B Shetty, Sharat Saxena, Nadira, Bindu, Helen, among others, to understand the dimension of the villains' empire. After all, it's our villains who make our protagonist the hero we all admire. An engrossing read, this book is for every film buff.Cover -- Contents -- Foreword -- Preface -- Acknowledgments -- Part I: Knowing the Baddies -- 1 - Who Are These Villains? -- Part II: The Baddies in Action -- 2 - The Fifties and Sixties -- 3 - Sholay and the Seventies -- 4 - The Eighties and After -- Part III: Empire of Evil and the Emperors -- 5 - That Other Self: The Vamps -- 6 - The Empire of Evil: Villains' Henchmen -- 7 - Those Dreadful Men -- 8 - The Unforgettable Baddies -- 9 - The End! -- About the AuthorBollywood Baddies is the first-of-its-kind book-length narrative of villainy in Hindi films. It discusses villains, vamps, and henchmen of Bollywood cinema, and also the actors who essayed such characters over the decades. The author discusses not just villains but also the evaluation of villainous characters vis-à-vis sociopolitical conditions in the country. The narrative begins with Ashok Kumar's negative role in Kismet as early as 1943, and goes up to the Agneepath remake (2012), where Sanjay Dutt plays Kancha Cheena, earlier essayed by Danny Denzongpa in the original. In between, it discusses all major villains, from Lala Sukhiram (Mother India) to Gabbar (Sholay) to "Lion" Ajit (Kalicharan) to Mogambo (Mr. India), and many others. While keeping villains in the focus, it also discusses popular henchmen and vamps, like M B Shetty, Sharat Saxena, Nadira, Bindu, Helen, among others, to understand the dimension of the villains' empire. After all, it's our villains who make our protagonist the hero we all admire. An engrossing read, this book is for every film buff.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
