62 research outputs found
Developing E. coli-E. coli co-cultures to overcome barriers of heterologous tryptamine biosynthesis
No full textTryptamine is an alkaloid compound with demonstrated bioactivities and is also a precursor molecule to many important hormones and neurotransmitters. The high efficiency biosynthesis of tryptamine from inexpensive and renewable carbon substrates is of great research and application significance. In the present study, a tryptamine biosynthesis pathway was established in a metabolically engineered E. coli-E. coli co-culture. The upstream and downstream strains of the co-culture were dedicated to tryptophan provision and conversion totryptamine, respectively. The constructed co-culture was cultivated using either glucose or glycerol as carbon source for de novo production of tryptamine.The manipulation of the co-culture strains’ inoculation ratio was adapted to balance the biosynthetic strengths of the pathway modules for bioproduction optimization. Moreover, a biosensor-assisted cell selection strategy was adapted to improve the pathway intermediate tryptophan provision by the upstream strain, which further enhanced the tryptamine biosynthesis. The resulting biosensor-assisted modular co-culture produced 194mg/L tryptamine with a yield of 0.02 g/g glucose using shake flask cultivation.The findings of this work demonstrate that the biosensor-assisted modular co-culture engineering offers a new perspective for conducting microbial biosynthesis.Peer reviewe
Prevalence Of Hypertension and Its Risk Factors Among Class III and Class IV Government Employees in Ahmedabad
No full textBackground: The developing country including India is experiencing epidemiological transition from communicable to Non communica- ble diseases and hypertension has emerged as a significant public health problem in both urban and rural areas. The present study was conducted to assess prevalence of Hypertension and its risk-factors in government employees of Ahmedabad city.
Methodology: It was a cross-sectional study conducted among 600 government employees of B.J. Medical College of Ahmedabad, Guja- rat. Data was collected using a pre-tested, questionnaire and Blood pressure was measured.
Results: Prevalence of hypertension was 17 % in males and 19.67% in females. Prevalence of hypertension was 53.28 % in age group of 55 years and above. 10.66 % males and 11.66 % females were found hypertensive on examination. Based on Odds Ratio, History of To- bacco consumption, family history of hypertension, low vegetables and fruits intake and higher waist circumference was associated with more risk of hypertension.
Conclusions: History of Tobacco consumption, family history of hypertension, low vegetables and fruits intake and higher waist cir- cumference increase the risk of hypertension
Enhanced Oil Recovery using Carbonated Polymeric Nanofluids : A New Age Approach to CO2 Utilization and Corrosion Mitigation
No full textThe author would like to thank the CIF facility and its staff, including Mr. Anuj Prajapati, Mr. Zahoor Alam, and Mr. Brijesh. Thanks are also extended to all the members associated with the work.Peer reviewe
Prevalence of Tobacco Use among Government Employees in Ahmedabad, Gujarat
No full textIntroduction: Tobacco is the most easily accessible addictive sub- stance which contributes to premature death and long term suffer- ing, being a major risk factor for non-communicable diseases. The objective was to study prevalence of tobacco usage and exposure to second hand smoke among the government employees.
Methodology: A cross sectional study was undertaken in urban area of Gujarat from January 2013 to November 2014. Total 300 males and 300 females were selected from class III and class IV government employees of B. J. Medical College and Civil Hospital, Ahmedabad for the study.
Results: In this study, 13% males were smoker and 33.67% males and 4.67% females were consuming smokeless tobacco products. Prevalence of smokeless tobacco was 26.36% in 35 to 45 years of age. Passive smoking was reported by 112 (37.33%) males and 70 (23.33%) in females. Prevalence of smoking was 22% in class IV employees compared to 4% in class III employees.
Conclusions: Prevalence of tobacco was significantly higher in class IV employees. Prevalence of smokeless tobacco consumption was higher in 35 to 45 years of age group and prevalence of smok- ing was higher in age group of 55 years and above
Design and Governance of Platforms to leverage an ecosystem of complementors
No full textThis paper analyzes how specific aspects of design and governance of platform explain complementors participation in the video game console industry. This study introduces a new dimension to measure platform openness and suggest that by opening the platform vertically to different markets, platform firms can smoothen complementors competition and incentivize them to participate in their network. This study makes a first attempt to measure platform boundary resources and highlight its positive influence on complementors participation. Finally, the analysis also explores conditions under which signing an exclusive contract is beneficial for complementors. Complementors tend to participate in exclusive contracts during the early stages of platform rather than mature stages due to intense competition among complementors in the latter stage. The results suggest that by following the correct design and governance strategies, platform firms can orchestrate a large network of complementors and proliferate a variety of complementary product offerings
A comparative study of metformin and glyburide in gestational diabetes mellitus
No full textBackground: Incidence of gestational diabetes mellitus (GDM) is increasing worldwide. GDM provides a window of opportunity for the primary prevention of the type 2 diabetes by preventing transgeneration transmission to fetus. Insulin in management of GDM has many drawbacks, so use of OHAs has been increased worldwide.Methods: Randomized control trial was performed in patients with GDM who required medical management. Subjects were randomized into two groups and treated with Metformin and Glyburide, results were compared.Results: While comparing efficacy of metformin and glyburide in this study for maternal variables; the failure rate of metformin was found to be 9.39 times higher compared to glyburide. Glyburide was associated with 9.5 times more risk to develop hypoglycemia in mother compared to metformin. While comparing neonatal variables nursery admission was found to be more and statistically significant in neonates whose mother has received glyburide compared to metformin (p=0.03, RR=2.26). Though statistically insignificant, LGA fetuses and neonatal hypoglycemia were 2.1 times more in glyburide group compared to metformin.Conclusions: Though glyburide can be effective alternative to metformin and insulin for GDM, it is associated with higher risk of maternal hypoglycemia, neonatal hypoglycemia, LGA fetuses and higher rate of nursery admissions compared to metformin. Higher adverse neonatal outcomes with glyburide use question the widespread use of glyburide as first line management modality in GDM and also as an alternative to insulin as advised by many groups.</jats:p
he Creators A Look at the Changing Work of Potters and the Future of Their Craft in Thimi, Nepal
No full textThe Newari sur-name Prajapati has been associated with those who are of the potter caste in the Kathmandu valley. In the past 30 years ceramics in the historic pottery town of Thimi has changed drastically from being an essential and necessary craft and the only occupation for Prajapatis, to a struggling population of visually aging potters. This paper examines the workshop Everest Pottery in Thimi nepal as a case study for the state of ceramics in Thimi today. The author traces the origins of the workshop\u27s founder Shiva Prajapati and examines the shift that Shiva made from traditional Newari pottery practices to modern techniques. The author discusses the success of Everest Pottery and the shift of their market from local to global. The author concludes that with the rise of aluminum, plastic and the fact that Nepali\u27s are no longer looking to buy terracotta pots, the traditional forms that have inhabited the houses of the Kathmandu Valley for generations may be all but gone in the next few years. As a secondary focus the author discovered that while Everest Pottery has left behind most traditional Newari pottery practices, Newari traditional values and customs remain present in the workplace and can be witnessed through the stark division of labor by gender. The women of Everest Pottery all have different stories of how they came to work in ceramics. The author discusses the stories of the women of Everest Pottery, their perceptions of their own roles in ceramics and the perceptions of the female role from the point of view of the men of Everest Pottery. The author found that while there is no spoken rule against women throwing on the wheel, even in a modern ceramic workshop, this tradition remains. Most women who learned to throw in their youth stop throwing when they get married, and additionally do not want to try again for fear that it is too difficult. Similarly, the men of Everest Pottery express doubts about women\u27s ability to throw due to their lack of strength. While the women of Everest Pottery seem to be happy with the status quo, the author offers a counter observation that while some women aren\u27t interested in the wheel, the ones that are given no space to learn. Additionally the author offers potential futures for ceramics if women are eventually included in this integral part of the process
Level of anxiety related to English language among general nursing and midwives students
No full textThe present study was conducted to assess the level of anxiety related to English language among General Nursing & Midwives Students. A descriptive research design was adopted to assess the level of anxiety related to English language among General Nursing & Midwives Students in selected nursing schools. 40 students were selected by Simple Random sampling technique. The study revealed that the most of the students had intermediate level of anxiety
Menstrual Hygiene: Knowledge and Practice Among Adolescent Girls of Rural Kheda District
No full textBackground: Menarche is not just a physiological phenomenon but it is a psychological, social as well as behavioral transition for an adolescent girl from girlhood to womanhood. Menstrual hygiene is most important, still neglected area of concern. Objective of study was to find out menstrual pattern, knowledge and practice among adolescent girls.
Method: A cross sectional study was undertaken in a rural area of Kheda district. Randomly selected 200 adolescent girls were inter- viewed using predesigned pretested questionnaire.
Results: It was evident that 47.5% participants were aware about menstruation before their menarche and the most important sources of information were mothers in 54% girls.10.5% respond- ents used only sanitary napkin during menstruation. 77% changed absorbent 1 to 2 times a day.
Conclusion: This study has highlighted the need of adolescent girls to have accurate and adequate information about menstruation
- …
