71 research outputs found
Acetaminophen confers neuroprotection during early cerebral ischemia-reperfusion
No full textStroke is the leading cause of disability in the United States. It usually occurs from a clot blocking a cerebral artery, resulting in cerebral ischemia and infarction. Few stroke therapeutics are available that definitively improve patient outcome. Acetaminophen is a readily available, inexpensive drug with a good safety profile - properties desirable of a neuroprotectant. We have previously shown acetaminophen to be effective following myocardial infarction. Since similar cell-destructive events occur during cerebral ischemia as in myocardial infarction, we sought to determine whether acetaminophen would protect neuronal cells in a similar fashion. Biochemical and molecular assays were employed to assess cell death and mitochondrial function. Acetaminophen significantly reduced cerebral infarct size, mitochondrial swelling and rupture as well as apoptosis. Acetaminophen also preserved mitochondrial membrane potential. Furthermore, acetaminophen prevented cerebral edema, as seen histopathologically. Taken together, these data suggest that acetaminophen is effective in preventing neuronal stroke damage.Ph.D.Includes bibliographical references (p. 95-114)by Sunanda S. Balig
Some Comments on the Question Whether Co-Occurrence Data Should Be Normalized
No full textIn a recent article in JASIST, L. Leydesdorff and L. Vaughan (2006) asserted that raw cocitation data should be analyzed directly, without first applying a normalization such as the Pearson correlation. In this communication, it is argued that there is nothing wrong with the widely adopted practice of normalizing cocitation data. One of the arguments put forward by Leydesdorff and Vaughan turns out to depend crucially on incorrect multidimensional scaling maps that are due to an error in the PROXSCAL program in SPSS.multidimensional scaling;PROXSCAL;Pearson correlation;author cocitation analysis;co-occurrence data;normalization
MONOBODY BINDING PROTEINS AS BIORECOGNITION ELEMENTS FOR ELECTROCHEMICAL BIOSENSORS
No full textThesis (Ph.D.)--Michigan State University. Chemical Engineering - Doctor of Philosophy, 2024The burden of poor prognosis and high mortality rates associated with complex and aggressive diseases can be reduced with early detection. Biomarker sensing provides a dynamic approach to early diagnosis. However, the lack of a single diagnostic biomarker that can be correlated to a specific disease, there is a need to create a biosensing platform that can detect multiple targets which vary in size and complexity. To address the need to use stable biorecognition elements for sensing, we have explored the utility of synthetic binding proteins, which are like antibodies in function, except much smaller in size. Small synthetic proteins derived from human fibronectin, also known as monobodies, can act as powerful and highly modular biorecognition elements. Using computational tools such as homology modeling and protein-protein docking, we have identified monobodies with a unique chemistry that have strong binding affinity for specific targets of interest. In this work, we have developed an innovative electrochemical biosensor that harnesses the modularity of monobodies for the detection of large biomolecules. We used lysozyme as our model target due to its clinical relevance, cost efficiency, and ease of availability. As these monobodies cannot inherently generate any signal on binding with the target, we have functionalized them using NHS-EDC chemistry and electrochemically grafted them on the surface of the electrode. These modifications help generate a readable signal when the biosensor comes into contact with the target of interest. Immobilization of the monobodies on the surface of the electrode has created a non-conductive layer that impedes electron transfer, thus enabling the selective detection of target molecules. Our findings indicate that this biosensor exhibits high specificity, negligible non-specific adsorption, and exceptional electrical stability, making it a promising tool for accurate biomolecule detection in complex physiological fluids like serum. This method offers the potential for multiplexing, enabling the creation of a versatile, adjustable biosensor that can support more accurate prognosis through detecting a range of disease-related biomarkers. The development of this novel protein-electrode interface opens exciting possibilities for improving the performance and reliability of portable diagnostic devices, with significant implications for clinical and analytical applications.Description based on online resource. Title from PDF t.p. (Michigan State University Fedora Repository, viewed ).Includes bibliographical references
Aplikasi Hydropuls Pada Rehabilitasi Sumur Air Tanah (Studi Kasus Rehabilitasi Sumur Air Tanah Bogor)
No full textPaper yang saya presentasikan pada Pertemuan Ilmiah Tahunan Ke-5 Perhimpunan Ahli Air Tanah Indonesia (PIT PAAI KE-5) 2021.
Judul : Aplikasi Hydropuls Pada Rehabilitasi Sumur Air Tanah (Studi Kasus Rehabilitasi Sumur Air Tanah Bogor)
Author : Sangka Aryawicaksona, Azwar Satrya Muhammad, Rudi Sunanda
Aplikasi Hydropuls Pada Rehabilitasi Sumur Air Tanah (Studi Kasus Rehabilitasi Sumur Air Tanah Bogor)
No full textPaper yang saya presentasikan pada Pertemuan Ilmiah Tahunan Ke-5 Perhimpunan Ahli Air Tanah Indonesia (PIT PAAI KE-5) 2021.
Judul : Aplikasi Hydropuls Pada Rehabilitasi Sumur Air Tanah (Studi Kasus Rehabilitasi Sumur Air Tanah Bogor)
Author : Sangka Aryawicaksona, Azwar Satrya Muhammad, Rudi Sunanda
A complex interplay between PGC-1 co-activators and mTORC1 regulates hematopoietic recovery following 5-fluorouracil treatment
No full textAbstractIn vitro stimulation of HSCs with growth factors generally leads to their depletion. Understanding the molecular mechanisms underlying expansion of HSCs in vivo following myeloablation could lead to successful expansion of HSCs ex vivo for therapeutic purposes. Current findings show that mTORC1 is activated in HSPCs following 5-fluorouracil treatment and that mTORC1 activation is dependent on mitochondrial ETC capacity of HSPCs. Moreover, expression of PGC-1 family members, proteins that regulate mitochondrial biogenesis, in HSPCs following 5-fluorouracil treatment changes; also, these proteins play a stage specific role in hematopoietic recovery. While PRC regulates HSCs' expansion during early recovery phase, PGC-1α regulates progenitor cell proliferation and recovery of hematopoiesis during later phase. During early recovery phase, PRC expression, mitochondrial activity and mTORC1 activation are relatively higher in PGC-1α−/− HSCs compared to WT HSCs, and PGC-1α−/− HSCs show greater expansion. Administration of rapamycin, but not NAC, during early recovery phase improves WT HSC numbers but decreases PGC-1α−/− HSC numbers. The current findings demonstrate that mTOR activation can increase HSC numbers provided that the energy demand created by mTOR activation is successfully met. Thus, critical tuning between mTORC1 activation and mitochondrial ETC capacity is crucial for HSC maintenance/expansion in response to mitogenic stimulation
CLEARING MISCONCEPTIONS ABOUT COOPERATIVE LEARNINGTHROUGH PERSONAL EXPERIENCES
No full textCooperative learning is a successful teaching strategy in which small teams, each with students of different levels of ability, use a variety of learning activities to improve their understanding of a subject. Each member of a team is responsible not only for learning what is taught but also for helping teammates learn, thus creating an atmosphere of achievement. Students work through the assignment until all group members successfully understand and complete it. The author has been using cooperative learning strategy for the last eight odd years. She has observed that although teachers in general have a positive opinion about cooperative learning they have their reservations regarding the hurdles which one may face while using cooperative learning strategy. Some of these are hurdles are due to misconception. The author through this paper tries to clear eight such misconceptions using her experiences with cooperative learning teaching strategy.</jats:p
An ortho-hydroxy-arylimine based probe: Fluorescence sensitivity towards Zn2+ ion
No full textDepartment of Chemistry, Jadavpur University, Kolkata-700 032, India
E-mail: [email protected]
Department of Chemistry, Raja Peary Mohan College, Uttarpara-712 258, Hooghly, West Bengal, India
Manuscript received online 15 September 2019, accepted 19 September 2019
A probe 6,6´-((1Z,1´Z)-(((propane-1,3-diylbis(oxy))bis(2,1-phenylene))bis(azanylylidene))bis-(methanylylidene))bis(2- ethoxyphenol) (H2L), is synthesized by the condensation of 2,2´-(propane-1,2-diylbis(oxy))dianiline with 3-ethoxy-2-hydroxybenzaldehyde and characterized by various spectroscopic techniques (1H NMR, FT-IR, ESI-MS etc.), exhibits greenish yellow emission (λem 556 nm) upon binding with Zn2+ ion in H2O-CH3 CN (1:9 v/v, HEPES buffer, pH 7.4) in a mixture of seventeen other biologically important metal ions. The limit of detection (LOD) is 15.5 nM. The 1:1 composition of the complex, Zn2+: L2–, is supported by Job’s plot, ESI-MS and 1H NMR measurements
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