167 research outputs found
Abstract B29: Effects of prenatal exposure to cancer treatment: The development of a placental murine xenograft model
No full textAbstract
The aim of this study was to develop a suitable murine xenograft model to examine the possible effect of drugs or chemotherapeutic agents on the placental tissue.
Methods: The test population consisted of 4 examination groups of six immunodefficient nude mice. After the mice were ovariectomised and hormonal stimulated (17b estradiol and progesterone pellets), placental tissue derived from first and third trimester pregnancies was engrafted subcutaneously bilateral in the interscapular fat pad. hCG (human chorionic gonadotrophin) secretion was measured in serum and urine by an enzyme-linked immunosorbent assay (ELISA). To examine the viability and preservation of the placental tissue, factors for proliferation (hCG, Ki67), apoptosis (cleaved caspase-3) and murine angiogenesis (CD31) were evaluated by immunohistochemistry (IHC). The expression level of genes relevant for the angiogenesis (IGF-1, PGF, IGF-2), inflammation (eNOS) and proliferation (Flk-1, Flt-1) was compared by using reverse transcription- quantitative real-time polymerase chain reaction (RT-qPCR).
Results: Comparing the placental tissue before and after engraftment, we found preserved structure and histological features. Also, the PCR analysis showed preserved genetic characteristics of the primary engrafted placental tissue. hCG secretion, evaluated by ELISA in the urine and blood, was present for 3 weeks.
Conclusion: We created a murine xenograft model with proven stability and preserved structure of the engrafted placental tissue for 3 weeks. This established murine xenograft model will be useful to examine the effect of cancer treatment; moreover, it could serve as a model for other fetal toxicity studies.
Citation Format: Magali Verheecke, Wout Devlies, Els Hermans, Frédéric Amant. Effects of prenatal exposure to cancer treatment: The development of a placental murine xenograft model. [abstract]. In: Proceedings of the AACR Special Conference: Patient-Derived Cancer Models: Present and Future Applications from Basic Science to the Clinic; Feb 11-14, 2016; New Orleans, LA. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(16_Suppl):Abstract nr B29.</jats:p
jDHBenelux Author Template
No full textThis repository contains the latest official GitHub hosted versions of the LaTeX template that authors are required to use when they finalize their contribtions to the DH Benelux Journal. The repository synchronises with the corresponding easy-to-use and well-documented Overleaf Template that provides authors with a low threshold environment for writing LaTeX – but can be used with any LaTeX compiler.
About this Release: Apart from some minor changes to the .cls, v2.0 introduces a number of new files to improve open source development with git and GitHub, including a README, a CC-BY 4.0 License, and a .gitignore file. It also prepares the repository for synchronisation with Zenodo, to improve sustainability.
Full Changelog: https://github.com/DHBenelux/jDHBenelux-author-template/compare/v1.1...v2.
Clinical Actionability of the Genomic Landscape of Metastatic Castration Resistant Prostate Cancer
No full textThe development of targeted therapies increases treatment options for metastatic castration resistant prostate cancer (mCRPC) patients. There is a need for strong predictive and prognostic signatures to guide physicians in treating mCRPC patients. In this review we unravel the possible actionability in the AR pathway, PI3K AKT signaling, and DNA repair pathways. Additionally, we make recommendations on biomarker trial design, and the clinical use of this new type of data.sponsorship: This research received no external funding. This research was supported by the 'Jozef De Wever' fund for prostate cancer prevention of the KU Leuven, Leuven, Belgium. ('Jozef De Wever' fund for prostate cancer prevention of the KU Leuven, Leuven, Belgium)status: Publishe
European Association of Urology Guidelines on Urological Infections: Summary of the 2024 Guidelines
No full textManaging Genital Herpes: A Mini-review for Urologists from the European Association of Urology Guidelines Panel for Urological Infections
No full textBridging the Babel of Textual Criticism
No full textPost by Wout Dillen, guest author for FonteGaia. Wout Dillen is a postdoctoral researcher at the University of Borås, Sweden, where his work is part of the DiXiT Marie Curie Initial Training Network. He developed the Lexicon of Scholarly Editing as an appendix to his Ph.D. dissertation, which was part of the ERC project CUTS (Creative Undoing and Textual Scholarship) at the University of Antwerp. Lexicographical Approaches to Encompassing a Multilingual Research Field. Although Textual Critic..
Classification of Urinary Tract Infections in 2025: Moving Beyond Uncomplicated and Complicated
No full textCase report on secondary testicular necrosis due to fulminant epididymitis: ultrasonographic evaluation and diagnosis
No full textBackground: Scrotal pain is a common complaint in the clinical practice, with many underlying causes. Infectious causes, like epididymitis, are frequently encountered in the work-up of scrotal pain. The presentation of epididymitis is mostly mild, yet major complications can occur. Case presentation: We present a 35-year-old male presenting with scrotal pain and swelling of the testicle. Epididymitis with testicular necrosis was diagnosed using repeated doppler ultrasonography measurements. An orchiectomy was performed which showed a hemorrhagic process with affected spermatic cord. Funiculitis together with epididymal swelling can impede testicular blood flow, with testicular necrosis possibly resulting in orchiectomy. This is the first case that proved funiculitis to co-exist in loss of colour doppler on pathological evaluation. Conclusions: In order to reduce major complications, medical therapy should be promptly initiated when there is a suspicion of epididymitis. Keywords: Testicular necrosis, Epididymitis, UTI, Complicationstatus: Published onlin
Urban underground space: Solving the problems of today’s cities
No full textAbstractThe world-wide trend of increased urbanisation creates problems for expanding and newly-developing cities alike. Population increase leads to an increased demand for reliable infrastructure, nowadays combined with a need for increased energy efficiency and a higher environmental awareness of the public. The use of underground space can help cities meet these increased demands while remaining compact, or find the space needed to include new functions in an existing city landscape. In many cases, underground solutions to urban problems are only considered if all other (above ground) options have been exhausted. When underground solutions are considered and evaluated from the planning or initial project stages onwards, more optimal solutions will become possible.Use of the underground is not limited to large scale infrastructure projects. This paper also shows innovative use of the underground for commercial and residential use, storage, water conveyance and treatment, and heritage conservation, and highlights how use of underground can bring more optimal solutions for urban development
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