24 research outputs found
Properties of Concrete Manufactured Using Steel Slag
No full textAbstractThis paper aims to study experimentally, the effect of partial replacement of coarse and fine aggregates by steel slag (SS), on the various strength and durability properties of concrete, by using the mix design of M20 grade. The optimum percentage of replacement of fine and coarse aggregate by steel slag is found. Workability of concrete gradually decreases, as the percentage of replacement increases, which is found using slump test. Compressive strength, tensile strength, flexural strength and durability tests such as acid resistance, using Hcl, H2SO4, and Rapid chloride penetration, are experimentally investigated. The results indicate that for conventional concrete, the partial replacement of fine and coarse aggregates by steel slag improves the compressive, tensile and flexural strength. The mass loss in cubes after immersion in acids is found to be very low. Deflection in the RCC beams gradually increases, as the load on the beam increases, for both the replacements. The degree of chloride ion penetrability is assessed based on the limits, given in ASTM C 1202. The viability of usage of SS in concrete is found
In vitro thrombolytic potential of bioactive compounds from marine Streptomyces sp. VITJS4
No full textThe most practical approach to reduce morbidity and mortality of coronary heart disease (CHD) is to delay the process of thrombus by usage of clot-dissolving agents. The necessities of such safer compounds are to be critically examined for thrombolytic activity especially, from marine sources. Thrombolytic agents have been investigated as a possible treatment for thrombus. The aim of this study was to investigate the in vitro thrombolytic potential of Streptomyces sp.VITJS4 (NCIM No. 5574); (ACC No: JQ234978.1) active compounds. The fibrin degradation revealed a clear transparent zone of clearance with 500μg/mL concentration showing 24mm hydrolysis. The thrombolytic effect of Streptomyces sp.VITJS4 compounds was also demonstrated in vitro clot lysis assay where The percent of thrombolysis by the crude extract showed 90±1.7% at the concentration of 1000µg/mL,  whereas percent of thrombolysis by streptokinase was found 100± 00%%. The bioactive compounds were further studied for spectrophotometric analysis. The UV-VIS profile showed different peaks ranging from 400-700 nm with different absorption respectively. The data confirmed the presence of both analogues with absorption maxima at 210 and 310 nm. A sensitive method using LC-MS technique was optimized for the separation and identification of bioactive metabolites which was indicated by the fingerprints. The results of the LC-MS analysis provided different peaks determining the presence of compounds with different therapeutic activities.  The current study refers the bioactive compound as impressive thrombolytic agent for further laboratory study. Further studies should be conducted to ensure the efficacy and safety of different concentration of bioactive compounds for drug development. Hence the results reported perhaps useful for the discovery of novel thrombolytic drugs from marine origin
Antibiotic Susceptibility Pattern of Uropathogens towards Nitrofurontoin & Nalidixic Acid: A Comparitive Study
No full textBacteriological Profile and their Antibiotic Susceptibility Pattern in Neonatal Bacteremia
No full textIn vitro Studies on Stimulation of Gymnemic Acid Production using Fungal Elicitor in Suspension and Bioreactor based Cell Cultures of Gymnema sylvestre R.Br.
No full textGymnemic acids have become a valuable drug in diabetes treatment due to their potent antidiabetic activity. These compounds are extracted commercially from large quantities of Gymnema sylvestre. Since the intact plant contains low concentrations of active compound, plant cell cultures have employed as an alternative to produce large amounts of these secondary metabolites. Moreover using a bioelicitor the secondary metabolite production can be increased. The objective of this study was to develop a rapid system for the enhanced production of gymnemic acid. Aspergillus niger cell extract was used as an elicitor to stimulate the production of secondary metabolite. Comparatively 9 fold increase of gymnemic acid yield was obtained in elicited cultures
Antimicrobial, Antioxidant and Cytotoxic Activity of MarineStreptomyces parvulus VITJS11 Crude Extract
No full textThe main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces parvulus VITJS11 with a view to assess their therapeutic potential. The biological activity of ethyl acetate extract was tested against fungal and bacterial pathogens. The free radical scavenging potential of the crude extract was determined by DPPH assay. The chemo preventive properties of S. parvulus VITJS11 ethyl acetate extract was examined by MTT assay on HepG2 cells. The morphological, physiological and the biochemical properties of the strain S. parvulus VITJS11 was confirmed by conventional methods. Genotypic characterization was done using 16S r-DNA partial gene amplification and sequencing. The authenticity of the crude chemical constitutes were determined by the GC-MS. The ethyl acetate extract of VITJS11 showed maximum antifungal activity against three Aspergillus species and prominent antibacterial activity against two Gram positive and Gram negative bacteria at 20 mg/mL. The antioxidant potential of the crude extract exhibited strong reducing power activity at 5mg/ mL with 85% inhibition and the cytotoxic effect was found with IC50 of 500µg/ mL on HepG2 cell lines. The GC-MS analysis and the chromatogram patterns revealed 16 peaks, indicating the presence of bioactive constituents, which included several important organic compounds, namely 9-(2',2'-dimethylpropanoilhydrazono)-3,6-dichloro-2,7-Bis-[2-(diethylamino)-ethoxy]fluorine (23.1) Dotriacontylpentafluoropropionate,(25.0) Octadecanoic acid, (20.0); Trans-2-methyl-4-n-butylthiane, S, S-dioxide.(19.0). The results showed the benefit of ethyl acetate extract from S. parvulus VITJS11 in treating microbial infections and indicated their broad spectrum of activity with beneficial virtues for therapeutic use
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY A N I N T E R N A T I O N A L J O U R N A L Antimicrobial, Antioxidant and Cytotoxic Activity of Marine Streptomyces parvulus VITJS11 Crude Extract
No full textABSTRAC
Bacteriological Profile and their Antibiotic Susceptibility Pattern in Neonatal Bacteremia
No full textNeonatal Bacteremia is the presence of live bacteria circulating in the blood of neonates. The bloodstream infections are important cause of neonatal mortality and morbidity. This study was done to know about the bacterial agents causing neonatal Bacteremia and their antibiotic sensitivity pattern. Blood cultures were done on clinically suspected bloodstream infections in neonates for a period of 18 months. 200 neonatal blood samples were cultured by conventional method. Bacterial isolates were identified and their antibiotic susceptibility testing were done. Out of 200 blood samples 88 samples(44%) showed positive blood culture. Blood culture was positive in 51 males(57.95%) and 37 females(42.1%).Among the positive blood culture samples, 66 bacterial isolates were Gram positive(75%) and 22 isolates were Gram negative(25%). Staphylococcus aureus(54.5%) was the most common bacteria isolated followed by coagulase negative Staphylococcus species(20.5%).Among the Gram negative organisms, E.coli was isolated in 13.6% of positive blood culture samples followed by Klebsiella pneumoniae(9.1%) and Pseudomonas sp.(2.3%). Gram positive organisms were highly resistant to oxacillin(88.8-89.61%).They were sensitive to Linezolid(93.75-94.5%),Teicoplanin(83.4-87.5%), Vancomycin(81.25-83.33%) and Amikacin(81.25-83.4%). Gram negative organisms were mostly resistant to third generation cephalosporins and gentamycin. They were mostly sensitive to amikacin and piperacillin. Prompt treatment of positive blood culture neonates with appropriate antibiotics can prevent the complications and can reduce the mortality
