195 research outputs found
Yash Chopra
No full textAs a charismatic director in the Indian film industry, Chopra’s name is synonymous with the glamour of the romantic film and a certain style within Indian culture. Spanning four decades, his directed features include some of the classic films of Indian film history, such as Deewaar and Kabhi Kabhie. His directorial career began in 1959 with Dhool Ka Phool and he has been a major producer since 1973, consolidating his success in the 1990s with a series of huge box office hits including Dilwale Dulhaniya Le Jayenge. He has also worked in other Hindi movie genres, directing action movies such as Mashaal and a thriller, Darr. This book discusses in depth his work with the Hindi megastar Amitabh Bachanan in films such as Deewaar, Trishul, Kala Patthar and Silsila and how, in his transformation of the look of mainstream cinema in Dil To Pagal Hai and other films, Yash Chopra has proved to be a tireless innovator within a mainstream tradition. The author integrates this analysis with information about the man and his work, based on interviews with Yash Chopra, his family, his colleagues, his stars, his contemporaries and major critics that include views from Amitabh Bachchan, Shahrukh Khan, Shashi Kapoor and Sri Devi. A study of a top contemporary Indian film director, Rachel Dwyer’s book also examines the influence on Chopra of predecessors such as Raj Kapoor and how his own legacy can be seen in such films as Kuch Kuch Hota Hai and younger directors such as Karan Johar and Aditya Chopra
First person – Kunal Chopra
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Kunal Chopra is first author on ‘Zebrafish duox mutations provide a model for human congenital hypothyroidism’, published in BIO. Kunal is a PhD student in the lab of Enrique Amaya at the University of Manchester, investigating reactive oxygen species in wound healing and regeneration
Problemas do discurso de Deepak Chopra: uma análise metalinguística de “A cura quântica”
Get PDFIn this article, our objective is to make some contributions to the debate around the “quantum mysticism” phenomenon, exploring discursive aspects that permeate an utterance with this kind of theme. To this end, we bring forth an analysis of the book Quantum healing by Deepak Chopra, based on the philosophy of the Bakhtin Circle. We investigate not only the author's argumentative strategies, but we also connect the constituent elements of this utterance (theme, structure and style) with its context of publication, production and reception. We end our arguments recognizing the incoherence that Chopra demonstrates with his intertwining of mystical and alternative conceptions with a scientific worldview, while uttering inconsistent metaphors and serious contradictions, but we also highlight that the author was able to influence the way in which concepts related to Quantum Physics circulate outside the academia.Neste artigo, nosso objetivo é contribuir para o debate sobre o misticismo quântico, explorando aspectos discursivos que permeiam um enunciado com tal tema. Para tanto, realizamos uma análise metalinguística do livro A cura quântica de Deepak Chopra, com base na filosofia da linguagem do Círculo de Bakhtin. Investigamos não apenas as estratégias argumentativas do autor, mas também conectamos os elementos constitutivos desse enunciado (tema, estrutura e estilo) com seu contexto de produção e publicação. A partir da análise reconhecemos a incoerência de Chopra ao sintetizar visões de mundo místicas e alternativas e uma visão de mundo científica repleta de metáforas inconsistentes e graves contradições, mas destacando como o autor conseguiu influenciar o modo como conceitos relacionados à Física Quântica são mobilizados fora do contexto acadêmico
Problemas do discurso de Deepak Chopra: uma análise metalinguística de “A cura quântica”
Get PDFIn this article, our objective is to make some contributions to the debate around the “quantum mysticism” phenomenon, exploring discursive aspects that permeate an utterance with this kind of theme. To this end, we bring forth an analysis of the book Quantum healing by Deepak Chopra, based on the philosophy of the Bakhtin Circle. We investigate not only the author's argumentative strategies, but we also connect the constituent elements of this utterance (theme, structure and style) with its context of publication, production and reception. We end our arguments recognizing the incoherence that Chopra demonstrates with his intertwining of mystical and alternative conceptions with a scientific worldview, while uttering inconsistent metaphors and serious contradictions, but we also highlight that the author was able to influence the way in which concepts related to Quantum Physics circulate outside the academia.Neste artigo, nosso objetivo é contribuir para o debate sobre o misticismo quântico, explorando aspectos discursivos que permeiam um enunciado com tal tema. Para tanto, realizamos uma análise metalinguística do livro A cura quântica de Deepak Chopra, com base na filosofia da linguagem do Círculo de Bakhtin. Investigamos não apenas as estratégias argumentativas do autor, mas também conectamos os elementos constitutivos desse enunciado (tema, estrutura e estilo) com seu contexto de produção e publicação. A partir da análise reconhecemos a incoerência de Chopra ao sintetizar visões de mundo místicas e alternativas e uma visão de mundo científica repleta de metáforas inconsistentes e graves contradições, mas destacando como o autor conseguiu influenciar o modo como conceitos relacionados à Física Quântica são mobilizados fora do contexto acadêmico
Management of alveolar defect with one-piece implant and index of gingival porcelain shade selection in a North Indian Youth
No full textEsthetics is a prime demand for patients who seek anterior replacement of missing teeth. Resorption of the alveolar bone of the edentulous area occurs if left untreated for a long time. It further leads to thinning of the labial cortical plate in the maxillary arch. Few other reasons for narrowing of the alveolar crest are anatomic such as congenitally missing teeth and pathologic, that is dental trauma from tooth avulsion. Nowadays, patients always look for a fixed alternative for the restoration of missing teeth. Other than a conventional fixed partial denture, dental implants are the first choice of treatment. It is one of the difficult tasks for the clinician as well as the dental technician to meet the expectation of the patient when the ridge defect is present in the edentulous area. Extensively invasive procedures such as guided bone regeneration for correction of alveolar defects lead to prolong treatment time and induce psychological trauma to the patient
Coming of age: growing up Muslim in Australia
No full textComing of Age: Growing up Muslim in Australia is a timely new book that explores the diversity within the many Muslim communities around Australia, through the personal stories of Muslim Australians – boxers, lawyers and authors amongst them.
Tasneem Chopra is chair of the Australian Women’s Centre for Human Rights and one of Melbourne’s most influential women. Irfan Yusuf is a commentator and author of Once Were Radicals: My Years As A Teenage Islamo-fascist. Together with their fellow panellists, they examine the worlds of sport, sex, religion, humour and society, as seen through an Islamic prism.
 
Characterization of lysine demethylase KDM5 family: Substrate specificity and identification of potential novel non-histone substrates
No full textA major regulatory influence over cell biology is lysine methylation and demethylation within histone proteins. The KDM5/JARID1 sub-family are 2-oxoglutarate and Fe(II)-dependent lysine-specific histone demethylases that are characterized by their Jumonji catalytic domains. This enzyme family is known to facilitate the removal of tri-/di-methyl modifications from lysine 4 of histone H3 (i.e., H3-K4me3/2), a mark associated with active gene expression. As a result, studies to date have revolved around KDM5's influence on disease through their ability to regulate H3-K4me2/3. Recently, evidence has demonstrated that KDM5's may influence disease beyond H3-K4 demethylation, making it critical to further investigate KDM5 demethylation of non-histone proteins. In efforts to help identify potential non-histone substrates for the KDM5 family, we developed a library of 180 permutated peptide substrates (PPS), with sequences that are systematically altered from the WT H3-K4me3 sequence. From this library, we characterized recombinant KDM5A/B/C/D substrate preference. Subsequently we developed recognition motifs for each KDM5 demethylase and used them to predict potential substrates for KDM5A/B/C/D. Demethylation activity was then profiled to generate a list of high/medium/low-ranking substrates for further in vitro validation for each of KDM5A/B/C/D. Through this approach, we analyzed prediction success rate and identified 66 high-ranking substrates in which KDM5 demethylases displayed significant in vitro activity towards. We further shown the ability to monitor changes in cellular methylation in a handful of the 66 high ranking candidate substrates in response to KDM5 inhibition. Specifically, we focused validation efforts on a high-ranking KDM5A novel substrate: p53-K370me3. We demonstrated significant recombinant KDM5A(1-588ΔAP) and KDM5A(1-801) activity towards the p53-K370me3 substrate in vitro. We then monitored KDM5A-mediated demethylation of the p53-K370me3/2 substrate in HCT 116 cells using a combination of wild-type KDM5A and inactive-mutant KDM5A(H483A) overexpression plasmids, along with immunoblotting, (co-) immunoprecipitation and mass spectrometry analysis. Furthermore, we have shown that KDM5A expression influences the established p53-53BP1 interaction. Finally, we identified a novel p53-TAF5 interaction dominated through the p53-K370me3 state and how KDM5A expression might affect this interaction. Ultimately, we have provided the first evidence of a KDM5 demethylase targeting a non-histone substrate for demethylation, via the novel KDM5A demethylation of the p53-K370me3 substrate
Identification of KDM3A Substrate Preference and Non-canonical Substrates Through Systematic and Rational Peptide Library Screens
No full textJumonji C (JmjC) lysine demethylases (KDMs) are a subfamily of Fe(II)/2-oxoglutarate-dependent dioxygenases which facilitate demethylation of lysine residues by catalyzing hydroxylation of methyl groups attached to the ε-amino group of lysine side chains. Although 24 JmjC KDMs have demonstrated demethylation activity, the current substrate space is largely restricted to several methylation sites within the histone code. However, the biological roles of these enzymes are increasingly being shown to also be attributed to interaction with non-histone proteins. Notably, KDM3A has become relevant to tumour progression due to recent findings of this enzyme’s role in promoting cancerous phenotypes, such as enhanced glucose consumption and upregulated mechanisms of chemoresistance. To aid in uncovering the mechanism(s) by which KDM3A, or other KDMs, impart their oncogenic function(s), this thesis aims to apply peptide library screening to identify novel in vitro substrates and reveal substrate specificity determinants. First, we developed a peptide quantification method to streamline workflows involving large peptide libraries. Second, we developed a general workflow to apply peptide permutation libraries to reveal KDM substrate specificity. Third, we applied a peptide permutation library of histone H3 di-methylated at lysine-9 (i.e., H3K9me2) to KDM3A and revealed (1) the substrate specificity profile of KDM3A and (2) three novel in vitro substrates of this enzyme. Finally, we applied a non-systematic peptide library based on a new hypothesis (a.k.a. Shared Specificity Hypothesis) and uncovered three novel in vitro substrates of KDM3A, of which one substrate was validated in tissue culture models. In compiling a list of all substrates uncovered in this thesis with the few previously known substrates, it became apparent that KDM3A most closely shares substrate specificity with the G9a methyltransferase. Therefore, we anticipate any expansion of the G9a substrate space will organically contribute to KDM3A substrate discovery. Overall, this thesis increased the KDM3A substrate space by 3-fold and revealed critical substrate specificity determinants of this enzyme
Granger Causality Detection via Sequential Hypothesis Testing
Get PDFMost of the metrics used for detecting a causal relationship among multiple
time series ignore the effects of practical measurement impairments, such as
finite sample effects, undersampling and measurement noise. It has been shown
that these effects significantly impair the performance of the underlying
causality test. In this paper, we consider the problem of sequentially
detecting the causal relationship between two time series while accounting for
these measurement impairments. In this context, we first formulate the problem
of Granger causality detection as a binary hypothesis test using the norm of
the estimates of the vector auto-regressive~(VAR) coefficients of the two time
series as the test statistic. Following this, we investigate sequential
estimation of these coefficients and formulate a sequential test for detecting
the causal relationship between two time series. Finally via detailed
simulations, we validate our derived results, and evaluate the performance of
the proposed causality detectors.Comment: 5 pages 3 figure
Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses
Get PDFSince HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-γ enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design
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