95 research outputs found
Karanjin, A Promising Bioactive Compound Possessing Anti-cancer Activity against Experimental Model of Non-small Cell Lung Cancer Cells
No full textAims: The aim of this study is to isolate the Millettia pinnata (Karanj) leaf extract for pure compound with anticancer properties and to study the molecular target of the isolates in non-small cell lung cancer cell lines. Background: In our earlier research Millettia pinnata leaf extract has demonstrated potential anticancer activities. Thus, in pursuit of the bioactive compounds, the most potential active extract from our previous study was purified. Furthermore, the anticancer properties of the isolated compound karanjin was studied and aimed for apopto-sis and restraining growth. Methods: A novel method was developed through column chromatography for isolation and purification of the compound karanjin from leaf chloroform extract. The purified component was then characterised using FTIR, mass spectrometry, and NMR. An MTT-based cytotoxicity assay was used to analyse cell cytotoxicity, whereas fluorescence staining was used for apoptosis and reactive oxygen species inhibition quantification. Furthermore, the real-time PCR assay was used to determine the molecular mechanism of action in cells causing cytotoxicity induced by karanjin dosing. Results: The anticancer activity of karanjin in A549 cell line exhibited prominent activity revealing IC50 value of 4.85 μM. Conferring the predicted molecular pathway study, karanjin restrains the proliferation of cancer cells through apoptosis, which is controlled by extrinsic pathway proteins FAS/FADD/Caspases 8/3/9. Down-regulation of KRAS and dependent gene expression also stopped cell proliferation. Conclusion: Karanjin has been identified as a compound with potential effect in non-small cell lung cancer cells. Molecular mechanism for apoptosis and inhibition of reactive oxygen species induced through H2O2 were observed, concluding karanjin have medicinal and antioxidant properties
Effect of neutron-irradiation on optical properties of SiO2-Na2O-MgO-Al2O3 glasses
No full textSilica based glasses are used as nuclear shielding materials. The effect of radiation on these
glasses varies as per the constituents used in these glasses. Glasses of different composition of SiO2-Na2OMgO-
Al2O3 were made by melt casting techniques. These glasses were irradiated with neutrons of different
fluences. Optical absorption measurements of neutron-irradiated silica based glasses were performed at room
temperature (RT) to detect and characterize the induced radiation damage in these materials. The absorption
band found for neutron-irradiated glasses are induced by hole type color centers related to non-bridging oxygen
ions (NBO) located in different surroundings of glass matrix. Decrease in the transmittance indicates the formation
of color-center defects. Values for band gap energy and the width of the energy tail above the mobility gap have
been measured before and after irradiation. The band gap energy has been found to decrease with increasing
fluence while the Urbach energy shows an increase. The effects of the composition of the glasses on these
parameters have been discussed in detail in this paper.Amanpreet Kaur Sandhu1, Surinder Singh1* and Om Prakash Pandey2
1Department of Physics, Guru Nanak Dev University, Amritsar-143 005, Punjab, India
2School of Physics and Materials Science, Thapar University, Patiala-147 004, Punjab, India
E-mail : [email protected] of Physics, Guru Nanak Dev University, Amritsar-143 005, Punjab, India
School of Physics and Materials Science, Thapar University, Patiala-147 004, Punjab, Indi
Studies on Optimization of Traditional Indian Savoury Snacks (Namkeen).
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Accelerating Population Balance Model - based particulate process simulations via parallel computing
No full textThe use of Population Balance Models (PBM) for simulating dynamics of particulate systems are inevitably limited at some point by the demands they place on computational resources. PBMs are widely used to describe the time evolutions and distributions of many industrial particulate processes, and its efficient and quick simulation would certainly be beneficial for process design, control and optimization. This thesis is an elucidation of how MATLAB's Parallel Computing Toolbox (PCT), a third-party toolbox called JACKET, and the MATLAB Distributed Computing Server (MDCS) may be combined with algorithmic modification of the PBM to speed up these computations on a CPU (Central Processing Unit), GPU (Graphics Processing Unit) and a computer cluster respectively. Parallel algorithms were developed for three dimensional and four dimensional population balance models incorporating hardware class-specific parallel constructs such as SPMD and gfor. Results indicate significant reduction in computational time without compromising numerical accuracy for all cases except for the GPU. The GPU seemed promising for larger problems despite its limitations of lower clock speeds and on-board memory compared to the CPU. Evaluations of the speedup and scalability further affirm the algorithms' performance.M.S.Includes bibliographical referencesIncludes vitaby Anuj Varghese Prakas
Study on cocoonase, sericin, and degumming of silk cocoon: computational and experimental
No full textAbstract Background Cocoonase is a proteolytic enzyme that helps in dissolving the silk cocoon shell and exit of silk moth. Chemicals like anhydrous Na2CO3, Marseille soap, soda, ethylene diamine and tartaric acid-based degumming of silk cocoon shell have been in practice. During this process, solubility of sericin protein increased resulting in the release of sericin from the fibroin protein of the silk. However, this process diminishes natural color and softness of the silk. Cocoonase enzyme digests the sericin protein of silk at the anterior portion of the cocoon without disturbing the silk fibroin. However, no thorough characterization of cocoonase and sericin protein as well as imaging analysis of chemical- and enzyme-treated silk sheets has been carried out so far. Therefore, present study aimed for detailed characterization of cocoonase and sericin proteins, phylogenetic analysis, secondary and tertiary structure prediction, and computational validation as well as their interaction with other proteins. Further, identification of tasar silkworm (Antheraea mylitta) pupa stage for cocoonase collection, its purification and effect on silk sheet degumming, scanning electron microscope (SEM)-based comparison of chemical- and enzyme-treated cocoon sheets, and its optical coherence tomography (OCT)-based imaging analysis have been investigated. Various computational tools like Molecular Evolutionary Genetics Analysis (MEGA) X and Figtree, Iterative Threading Assembly Refinement (I-TASSER), self-optimized predicted method with alignment (SOPMA), PROCHECK, University of California, San Francisco (UCSF) Chimera, and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) were used for characterization of cocoonase and sericin proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), protein purification using Sephadex G 25-column, degumming of cocoon sheet using cocoonase enzyme and chemical Na2CO3, and SEM and OCT analysis of degummed cocoon sheet were performed. Results Predicted normalized B-factors of cocoonase and sericin with respect to α and β regions showed that these regions are structurally more stable in cocoonase while less stable in sericin. Conserved domain analysis revealed that B. mori cocoonase contains a trypsin-like serine protease with active site range 45 to 180 query sequences while substrate binding site from 175 to 200 query sequences. SDS-PAGE analysis of cocoonase indicated its molecular weight of 25–26 kDa. Na2CO3 treatment showed more degumming effect (i.e., cocoon sheet weight loss) as compared to degumming with cocoonase. However, cocoonase-treated silk cocoon sheet holds the natural color of tasar silk, smoothness, and luster compared with the cocoon sheet treated with Na2CO3. SEM-based analysis showed the noticeable variation on the surface of silk fiber treated with cocoonase and Na2CO3. OCT analysis also exemplified the variations in the cross-sectional view of the cocoonase and Na2CO3-treated silk sheets. Conclusions Present study enlightens on the detailed characteristics of cocoonase and sericin proteins, comparative degumming activity, and image analysis of cocoonase enzyme and Na2CO3 chemical-treated silk sheets. Obtained findings illustrated about use of cocoonase enzyme in the degumming of silk cocoon at larger scale that will be a boon to the silk industry
<b style="">Seed fume of <i style="">Solanum surattense</i>: A traditional panacea</b> <b style="">for teeth and gums</b>
No full text206-207
This communication
provides a brief account of a time-tested remedy from seed fume of Solanum surattense Burm.. f. against
tooth and gum disorders. The practice is quite popular among the aborigines of
Gonda, Bahraich and Balrampur districts of Uttar Pradesh, India.
The text deals with method of treatment and results of clinical trial conducted
by author himself.
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Growth and morphological evolution of c-axis oriented AlN films on Si (100) substrates by DC sputtering technique
No full text
Fig 5 -
No full textEffect of inoculation with bacterium ZNP-4 on plant growth and chlorophyll contents under different metal (ZnSO4) concentrations, (A) shoot length (B) root length (C) fresh weight (D) dry weight (E) chlorophyll a (F) chlorophyll b. Values are mean±SD of triplicate sets (n = 15). * represent the significant difference as compared to respective control according to Ducan’s multiple range test (p = 0.05).</p
Fig 3 -
No full textGrowth curve of ZNP-4 under different conditions (A) varying concentration of NaCl (B) different temperature (C) different pH (D) ZnSO4 concentrations. Each value represent the mean of three replicates±SD (n = 3).</p
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