228 research outputs found
Fifth National Climate Assessment: Chapter 24 Midwest
No full textBaker, John M.; Wilson, Aaron B.; Ainsworth, Elizabeth A.; Andresen, Jeffrey; Austin, Jay A.; Dukes, Jeffrey S.; Gibbons, Elizabeth; Hoppe, Brendalynn O.; LeDee, Olivia E.; Noel, James; Roop, Heidi A.; Smith, Sara A.; Todey, Dennis P.; Wolf, Ray; Wood, Jeffrey D.. (2023). Fifth National Climate Assessment: Chapter 24 Midwest. Retrieved from the University Digital Conservancy, https://doi.org/10.7930/NCA5.2023.CH24
Characterization of a Murine Keratin Gene
No full textiv, 40 p.Keratins are a group of related proteins that are the major
differentiation product of epidermal cells. The mass of keratin
proteins ranges from 40 to 70 kiloDaltons. Keratins of 55, 59 and
67 kDal. are the major protein products of newborn mouse epidermis.
Several studies have' found unusual sized keratin proteins in diseased
and cancerous epidermal cells. Very little is known (published)
about the structure of keratin genes. This study involved the
isolation and characterization of the 59 kDal. keratin gene.
Through the use of 32 p-labeled cDNA (DNA complementary to the
59 kDal. keratin mRNA), fragments containing translated material
were screened from the mouse genomic library. These translated DNA
fragments were isolated from their lambda phage particles, recloned
into plasmid pBR 322, and characterized using restriction enzymes.
The results indicate that the 59 kDal. keratin gene contains sections
of untranslated(intron) DNA that separate the translated regions.National Cancer Institute. National Institutes of Health. Bethesda, Maryland
[Photograph 2012.201.B1136.0026]
No full textPhotograph used for a story in the Oklahoma Times newspaper. Caption: "Pfc. John Roop, left, and Spec. 4 Dennis Bergstrom, both of Tulsa, wouldn't let Maj. Gen. L. E. Weber, Oklahoma's adjutant general, off the Ft. Chaffee reservation Thursday night because he did not have the proper papers.
Isolation of Natural Keratin Genes from a Mouse Gene Library
No full textiv, 29 p.Complementary DNA (cDNA) was synthesized to total poly(A) RNA (mRNA) isolated from newborn mouse epidermis and used to construct recombinant plasmids. Recombinants containing cDNA inserts corresponding to the 67 Kd, 59 Kd, and 55 Kd keratins, which are the major keratins synthesized in this tissue have been identified. By RNA blot analysis from newborn mouse epidermis, RNA species have been identified that are approximately 2400, 2000, and 1800 nucleotides in length and are complementary to the 67 Kd, 59 Kd, and 55 Kd cDNA's respectively.
Using these cDNA's as probes, this project entails the isolation and characterization of the natural keratin genes corresponding to the 67 Kd, 59 Kd, and 55 Kd keratin· proteins. The source of the natural genes was a mouse gene library recombinantly constructed in bacteriophage vector Charon 28.National Cancer Institute. Bethesda, Maryland
Rising Seas: Representations of Antarctica, Climate Change, and Sea Level Rise in U.S. Newspaper Coverage
No full textData are stored in separate .csv/Excel files. All information identifying the coders of this data has been removed.A changing Antarctica carries large implications for global climate systems and sea level rise. However, how climate change is altering Antarctica and how these changes and their relevance are communicated in news media remains unclear. This study explores how Antarctica, climate change, and sea level rise are reported in news media by conducting a content analysis of Antarctic climate coverage in seven U.S. newspapers between March 2007 and December 2022. Findings suggest that newspaper reporting of Antarctica’s changing climate is limited, and that framed coverage about Antarctica, climate change, and sea level rise primarily emphasizes scientific and ecological implications.
Data used to conduct this study include:
1) A spreadsheet detailing article metadata for the 987 texts analyzed in this study. Details include the headline, outlet, author, data of publication, and ID number assigned to each article.
2) A spreadsheet containing the results of a content analysis of the 987 texts examined in this study. Analytical reliability was statistically assessed using a two-person inter-coder reliability process.Bruns, C.J.; Huffman, D.R.; Neff, P.D.; Timm, K.M.F.; Roop, H.A.. (2024). Rising Seas: Representations of Antarctica, Climate Change, and Sea Level Rise in U.S. Newspaper Coverage. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/265195
Loricrin at the Boundary between Inside and Outside
No full textCornification is a specialized mode of the cell-death program exclusively allowed for terrestrial amniotes. Recent investigations suggest that loricrin (LOR) is an important cornification effector. As the connotation of its name (“lorica” meaning an armor in Latin) suggests, the keratin-associated protein LOR promotes the maturation of the epidermal structure through organizing covalent cross-linkages, endowing the epidermis with the protection against oxidative injuries. By reviewing cornification mechanisms, we seek to classify ichthyosiform dermatoses based on their function, rather than clinical manifestations. We also reviewed recent mechanistic insights into the Kelch-like erythroid cell-derived protein with the cap “n” collar homology-associated protein 1/nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway in skin health and diseases, as LOR and NRF2 coordinate the epidermis-intrinsic xenobiotic metabolism. Finally, we refine the theoretical framework of cross-talking between keratinocytes and epidermal resident leukocytes, dissecting an LOR immunomodulatory function
Altering Cell Fate: From Thymus Epithelium to Skin Stem Cells
No full textIn a recent study in Nature, Bonfanti et al. (2010) report that the skin microenvironment can convert thymic epithelial cells into skin stem cells. This finding suggests that somatic stem cells may have a broader potential for lineage switching than previously thought
Mouse Models in Preclinical Studies for Pachyonychia Congenita
No full textThe similarities between the human and mouse genomes often allow researchers to make accurate predictions about the roles of their human counterparts. Because of the similar physiology between these two mammals, mice are used extensively in the laboratory to investigate the mechanisms of human diseases. Furthermore, mice provide us with the option of testing the toxicity of drugs and the safety of therapeutic approaches prior to human application. Here, we review the existing mouse models involving the keratin genes (K6a, K6b, K16, and K17) that cause the human genetic disorder pachyonychia congenita (PC). We also suggest methods to more accurately model this autosomal dominant skin condition in the mouse in order to better understand the pathophysiological processes underlying PC and importantly, provide a test-bed for testing emerging therapies in vivo
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