1,690 research outputs found
Letter from Lea Perry to Kazuo Ito, November 1943
No full textTranscript of a letter from Lea Perry to Kazuo Ito. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0030.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Letter from Lea Perry to Kazuo Ito, May 22, 1942
No full textTranscript of a letter from Kazuo Ito to Lea Perry. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0005.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Letter from Lea Perry to Kazuo Ito, November 27, 1942
No full textTranscript of a letter from Lea Perry to Kazuo Ito. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_9024.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Letter from Lea Perry to George Ito, October 22, 1942
No full textTranscript of a letter from Lea Perry to George Ito. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0018.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Letter from Lea Perry to Kazuo Ito, August 11, 1943
No full textTranscript of a letter from Lea Perry to Kazuo Ito and family. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0048.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Letter from Lea Perry to Kazuo Ito, November 30, 1942
No full textTranscript of a letter from Lea Perry to Kazuo Ito. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0025.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
The River Lea 1571-1767: a river navigation prior to canalisation
No full textPhDIn pre-industrial England river navigations were subject
to improvement by canalisation, the introduction of
artificial navigation cuts and pound locks. Along the Lea
this did not happen until 1767. Before that the navigation,
except for one short period, relied upon a less efficient
technology, the provision of flashes from fishing weirs,
turnpikes and mills. Yet the river was still an important
transport route, particularly for the supply of grain, meal
and malt to London. It had been this during the mediaeval
period, but not by the middle of the sixteenth century. Then
in 1571 the City of London sponsored legislation to construct
a canal from the Lea to London. Parliamentary opposition
thwarted the original ambitious scheme, so two cheaper,
shorter canals were considered, but never built. Instead
an ambitious and unique river improvement scheme was
successfully implemented. This experimental navigation
(reducing reliance on flashes to a minimum) survived 20
years, before persistent and violent opposition from land
carriers closed it. A Star Chamber case upheld the rights
of the bargemen, but the experimental navigation was not
restored. Instead the traditional flash-lock navigation
re-appeared, and was to last, with only minor improvementg
until 1767. In the intervening years the navigation
continued to expand and prosper., This despite the admitted
problems of relying on flashes and tides, and despite a
series of major disputes with the New River Companyq the
millers, fishermen and riparian land-owners. Conflict there
certainly was, but also compromise. Ultimately all parties
were prepared to accept the conflicting rights of other
users, provided they could defend their own. commissions
of Sewers provided an effective administrative forum to
effect and authorise such compromise, even after the
appointment of a body of Trustees in 1739. That the Lea
was an adequate navigation before canalisation, despite
a 'second-best' technology and an unpaid part-time administrative
structure means' that a valid comparison with the
concept of Appropiate Technology, discussed in modern-day
development theory, is possible
Letter from Lea Perry to Kazuo Ito and family, November 16, 1943
No full textTranscript of a letter from Lea Perry to Kazuo Ito and family. The original letters are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL, December 2014. Digital reproduction of the original item is found in item: ssu_nbea_0052.The North Bay Ethnic Archive features material related to the forced relocation of northern San Francisco Bay Area residents to the Granada (Amache) incarceration camp, Colorado. It includes correspondence, photographs, and reports. Some of the original items are housed with the Sonoma County Japanese American Citizens League (JACL), and were borrowed for digitization courtesy of the JACL. The remainder are housed in Special Collections
Optimization of extraction and MPS methods to generate forensic-quality mtDNA sequences
No full textDNA extractions from forensically difficult specimens, such as hair, often prove to be difficult, as the specimens may be severely degraded due to age and various environmental influences. Several methods are already available in forensics to extract difficult samples (such as teeth or bones), but none of these methods have been adapted to challenging and ancient hair samples. Here, the protocol of Dabney & Meyer, 2019 was adapted to hair and the amount of Binding Buffer was altered to successfully extract mitochondrial (mtDNA) and nuclear DNA (nDNA) from aged degraded hair samples. The quantity and quality of the extracted DNA could be increased several times compared to conventional extraction methods. Furthermore, the complete mitochondrial genome was successfully sequenced from the extracted DNA using Massive Parallel Sequencing (MPS). MPS methods are very sensitive to highly degraded mtDNA fragments. By using Precision ID mtDNA Whole Genome Panel (P-ID), fragments exceeding 150 bp could be sequenced in the whole mitochondrial genome and by using Capture Hybridization and Primer Extension Capture (PEC) fragments larger than 50 bp could even be sequenced in the Control Region (CR), which could be found mainly in ancient hair samples. With the use of P-ID or PEC, the obtained haplotypes could be assigned to haplogroups, providing evidence for the matrilineal origin of individuals. Overall, the results of this study can contribute to routine forensic laboratories and facilitate the processing and extraction of highly degraded hair samples.written by: Lea DemetzMasterarbeit Universität Innsbruck 2022Masterarbeit Medizinische Universität Innsbruck 202
Optimization of extraction and MPS methods to generate forensic-quality mtDNA sequences
No full textDNA extractions from forensically difficult specimens, such as hair, often prove to be difficult, as the specimens may be severely degraded due to age and various environmental influences. Several methods are already available in forensics to extract difficult samples (such as teeth or bones), but none of these methods have been adapted to challenging and ancient hair samples. Here, the protocol of Dabney & Meyer, 2019 was adapted to hair and the amount of Binding Buffer was altered to successfully extract mitochondrial (mtDNA) and nuclear DNA (nDNA) from aged degraded hair samples. The quantity and quality of the extracted DNA could be increased several times compared to conventional extraction methods. Furthermore, the complete mitochondrial genome was successfully sequenced from the extracted DNA using Massive Parallel Sequencing (MPS). MPS methods are very sensitive to highly degraded mtDNA fragments. By using Precision ID mtDNA Whole Genome Panel (P-ID), fragments exceeding 150 bp could be sequenced in the whole mitochondrial genome and by using Capture Hybridization and Primer Extension Capture (PEC) fragments larger than 50 bp could even be sequenced in the Control Region (CR), which could be found mainly in ancient hair samples. With the use of P-ID or PEC, the obtained haplotypes could be assigned to haplogroups, providing evidence for the matrilineal origin of individuals. Overall, the results of this study can contribute to routine forensic laboratories and facilitate the processing and extraction of highly degraded hair samples.written by: Lea DemetzMasterarbeit Universität Innsbruck 2022Masterarbeit Medizinische Universität Innsbruck 202
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