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Genotoxic effects in zebrafish (Danio rerio) erythrocytes of two pharmacological compounds in an impacted Italian river
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DNA damage in eelpout (Zoarces viviparus) from Goteborg harbour
No full textThe relationship between DNA damage and the exposure of marine organisms to environmental contaminants was
examined in the Goteborg harbour area. This research is part of a wider ecotoxicological study planned to evaluate the biological
impact of chemical contamination in the River Gota estuary, following a bunker oil (10-100 tonnes) spill occurred in June 2003.
Here we present data on the DNA strand breaks derived using the comet assay and the presence of apoptotic cells using the
diffusion assay in nucleated erythrocytes of the eelpout (Zoarces viviparus) from the study area and at a clean reference site.
Polycyclic aromatic hydrocarbon metabolites were also analyzed in the bile of exposed fish. The results showed a high level of
damaged DNA, paralleled by a peak in bile PAH metabolites, in fish from the most impacted site, 3 weeks after the oil spill. A
significant recovery was observed in specimens from the spill site, 5 months later, but not in fish caught in the middle part of
Goteborg harbour, which is chronically subjected to heavy chemical pollution. The levels of apoptic cells did not show any marked
variations, but a significant recovery was observed in fish from the oil impacted site 5 months after the spill
Cellular responces in mussels (Mytilus galloprovincialis) transplanted to the estuary of Cecina river
No full textCellular biomarkers for monitoring estuarine environments: transplanted versus native mussels.
No full textAbstract
In developed countries, estuarine environments are often subjected to chemical pollution, whose biological impact is profitably evaluated by the use of multi-biomarker approaches on sentinel species. In this paper, we investigate genotoxicity and lysosomal alterations in the Mediterranean mussel (Mytilus galloprovincialis), from the estuary of the River Cecina (Tuscany, Italy), selected as “pilot basin” within theWater Frame Directive (2000/60 European Community). Both native and 1 month transplanted mussels were used in order to compare these two approaches in terms of sensitiveness of specific biomarker responses. Genotoxic effects were evaluated as strand breaks, by single cell gel electrophoresis (or Comet assay), and as chromosomal alterations, by the micronucleus test in gill cells. Lysosomal alterations were assessed by the neutral red retention time (in haemocytes), lipofuscin accumulation and ultrastructure (in digestive cells). Heavy metal bioaccumulation was also analysed. Mussels from the River Cecina showed a general alteration of all the biomarkers investigated, accompanied by an elevation of tissue metal levels. However, some differences in specific responses occurred between transplanted and native mussels. Early biomarkers, such as those based on DNA and lysosomal membrane integrity, were induced at similar degree in native and transplanted mussels; while alterations resulting from cumulative events, as the increase of micronuclei frequency were much more elevated in native specimens (23.1±7.6) than in transplanted (9.3±4.7) and reference ones (5.8±5.2). Similarly, the comparison between lipofuscin accumulation and mean lysosomal diameter in impacted and control sites, gave significant differences exclusively with transplanted mussels. These results suggest that the parallel use of caged and native mussels in environmental biomonitoring can improve the characterization of the study area
Genotoxicity biomarkers for monitorating freshwater enviroments. The case of an impacted Italian river
No full textDNA damage in the eelpout (Zoarces viviparus) from Göteborg harbor: a comet assay evaluation
No full textCellular responses in mussels (Mytilus galloprovincialis) transplanted to the estuary of an impacted Italian river
No full textCellular responses to human impact at the estuary of a small italian river. An investigation with transplanted marine mussels (Mytilus galloprovincialis)
No full textCellular responces in mussels (Mytilus galloprovincialis) transplanted to the estuary of an impacted italian river
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