52 research outputs found
Optimization of Xylanase Production from Bacillus Sp. Pkd-9 under Solid State Fermentation, Partial Characterization and Application
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Corrigendum: Thalassemia, biobanking infrastructures, and personalized stem cell therapies in Chennai
No full textA Corrigendum onThalassemia, biobanking infrastructures, and personalized stem cell therapies in Chennaiby Panwar, A. (2023). Front. Sociol. 8:1057220. doi: 10.3389/fsoc.2023.1057220In the published article, there was an error in the Funding statement. The Funding statement displayed did not acknowledge UKRI/NERC funding during preparation of the article. The correct statement is “The author also acknowledges support from the NERC grant (NE/T013230/1) during preparation of this article.” The correct Funding statement appears below.<br/
Understanding the molecular basis of prebiosis of manno-oligosaccharides in probiotic Lactobacillus sp.
No full textTranscription analysis of galactomannooligosaccharides utilization by Lactobacillus plantarum WCFS1.
No full textPlantderivedgalactomannooligosaccharides(GMOS)areanemergingclassofprebiotics,butnoinformationis available on theirutilization in lactobacilli atthe molecular level. Thecurrent studyaimed atidentifying the geneticlociinvolvedinthetransportandcatabolismoflocustbeangumderivedGMOSinLactobacillusplantarum WCFS1. Substrate depletion study showed that L. plantarum WCFS1 can metabolize only short chain GMOS (degreeofpolymerization;DP≤3).GlobaltranscriptomemicroarrayprofilingofL.plantarumWCFS1revealed differential expression when GMOS or control sugars (glucose, galactose, and mannose) were used as a sole carbohydratesource.Twogeneticlociinvolvedincellobiose(~3.2kb)andoligo-sucrose(~7.3kb)utilizationin L. plantarumWCFS1werehighlyup-regulatedupto8.3 andupto6.7-fold,respectivelyby GMOSutilization. qRT-PCR studies of the selected gene clusters showed correlation with microarray data. Altogether, transcriptomeandqRT-PCRstudiesofL.plantarumWCFS1suggestedthatun-substitutedmannobiose(DP2)mightbe metabolized by proteins encoded by the cellobiose operon while, substituted DP2 (galactomannose) and DP3 (galactomannobiose)weremostlikelytransportedandcatabolizedbytheoligo-sucroseutilizationlociencoded proteins
Enhanced survival of Lactobacillus sp. in β‐manno‐oligosaccharides‐enriched low‐fat ice cream under simulated gastrointestinal stress.
No full textSurvival of probiotic Lactobacillus plantarum NCIM 2372 and Lactobacillus fermen‐
tum NCIM 2165 was studied in low‐fat ice creams enriched with prebiotic β‐manno‐
oligosaccharides (β‐MOS) under simulated gastro‐intestinal stress. β‐MOS‐enriched
synbiotic ice creams had better overrun values (up to 23%), brightness (up to 78.06),
whiteness index (up to 72.3), consistency index (up to 1.11 Pa.sn
), flow behavior (up to
0.67), and viscosity (up to 0.22 Pa.s) than low fat control. Viability of probiotic cultures
in β‐MOS‐enriched ice cream during storage period showed insignificant (p > 0.05)
reduction till 40th day and marginal reduction at 65th day (up to 1.4 log CFU/ml).
In vitro gastro‐intestinal stress study of stored ice creams (−20°C, till 65 days) re‐
vealed that β‐MOS enriched ice cream enhanced L. plantarum and L. fermentum sur‐
vival under gastric stress (up to 2.8 log CFU/ml less reduction) and bile stress (up to
3.12 log CFU/ml less reduction) when compared with respective controls.
Practical applications
The growth and survival of probiotic bacteria in functional food products under gas‐
tro‐intestinal stress conditions holds utmost importance to deliver health benefits.
Low‐fat dairy products enriched with prebiotics to support the probiotic bacteria\ud
appear as a viable strategy to cater the requirements of health inclined consumers.
Our studies demonstrated not only the effectiveness of β‐manno‐oligosaccharides
(β‐MOS) in improving the survival of Lactobacillus sp. but also enhancement of the
functional properties of low‐fat ice creams. β‐MOS appears as suitable candidates for
the development of low‐fat dairy‐based functional food products
Cross-linked enzyme aggregates (CLEAs) and magnetic nanocomposite grafted CLEAs of GH26 endo-β-1,4-mannanase: Improved activity, stability and reusability
No full textProduction, extraction and characterization of alkaline xylanase from Bacillus sp. PKD-9 with potential for poultry feed
No full textBacillus sp. PKD-9, isolated from the nest of mud dauber (Sceliphron caementarium) at CSIR-CFTRI, Mysore,
produced tremendous amount of xylanase [75,000 IU/g dried fermented wheat bran (IU/g DFWB)] after 72 h
under solid state fermentation using wheat bran as both carbon and nitrogen source at 37 1C, pH 8.0.
Optimization of operational conditions for xylanase extraction based on contour plots and variance analysis
using response surface methodology (RSM) further enhanced xylanase yield by 1.30-fold (98,0000 IU/g
DFWB). Biochemical characterization revealed that xylanase was optimally active at pH 8.0 and 50 1C with
half-lives of 60 and 30 min at 45 and 50 1C, respectively. It exhibited remarkable stability (more than 75.0%
activity) over a broad pH range (6.0–10.0) after 25 h of incubation at room temperature and even in the
presence of known inhibitors (iodoacetamide, iodoacetic acid and β-mercaptoethanol). The release of reducing
sugars from poultry feed after xylanase digestion suggested its potential in improving feed digestibility
Recombinant endo-mannanase (ManB-1601) production using agro-industrial residues: Development of economical medium and application in oil extraction from copra
No full textExpression of pRSETA manb-1601 construct in Hi-Control Escherichia coli BL21 (DE3) cells improved
recombinant endo-mannanase (ManB-1601) production by 2.73-fold (1821 ± 100 U/ml). A low-cost,
agro-industrial residue supplemented industrial medium for enhanced and economical production of
ManB-1601 was developed in two mutual phases. Phase-I revealed the potential of various pre- (induction
time: 5 h, induction mode: lactose 0.5 mM) and post-induction [peptone supplementation: 0.94%
(w/v), glycerol 0.123% (v/v)] parameters for enhanced production of ManB-1601 and resulted in 4.61-
fold (8406 ± 400 U/ml) and 2.53-fold (3.30 g/l) higher ManB-1601 and biomass production, respectively.
Under phase-II, economization of phase-I medium was carried out by reducing/replacing costly ingredients
with solubilized-defatted flax seed meal (S-DFSM), which resulted in 3.25-fold (5926 U/ml) higher
ManB-1601 production. Industrial potential of ManB-1601 was shown in oil extraction from copra as
enzyme treatment led to cracks, peeling, fracturing and smoothening of copra, which facilitated higher
(18.75%) oil yield
Cross-linked enzyme aggregates (CLEAs) and magnetic nanocomposite grafted CLEAs of GH26 endo-Beta-1,4-mannanase: Improved activity, stability and reusability.
No full textA comparative study on immobilization of recombinant endo-β-1,4-mannanase (ManB-1601),
using cross-linked aggregated form (MB-C) and novel chitosan magnetic nanocomposites of
MB-C (MB-Mag-C) was carried out. FT-IR and Raman spectroscopy were used to confirm the
surface modifications while, scanning electron and atomic force microscopy were performed to
demonstrate the surface topology and magnetic nature of MB-C and MB-Mag-C. Among MB-C
and MB-Mag-C, the former showed better activity and stability in broad range of pH, thermostability
and kinetic parameters while, the latter showed higher temperature optima and solvent
stability. MB-C and MB-Mag-C when compared with free enzyme showed up to 73.2% higher
activity (pH 4-9), up to 95.6% higher stability (pH 3-10, 9 h incubation at room temperature), up
to 15 oC higher optimal temperature, higher stability (up to 83%) in the presence of solvents and
up to 1.62-fold higher deactivation energy (Ed). Immobilized enzymes were able to repeatedly
hydrolyze locust bean gum till 12 cycles and generated predominantly di-, tri- and tetra- species
of β-manno-oligosaccharides
Complex alpha and beta mannan foraging by the human gut bacteria
No full textThe human gut microbiota (HGM), a community of trillions of microbes, underscores its contribution by impacting many facets of host health and disease. In the HGM, Bacteroidota and Bacillota represent dominant bacterial phyla, which mainly rely on the glycans recalcitrant to host digestion to meet their energy requirements. Accordingly, the impact of dietary and host-derived glycans in the assembly and operation of these dominant microbial communities continues to be an area of active research. Among various glycans, mannans represent an integral component of the human diet. Apart from their health effects, the diverse and complex
mannan structures bears molecular signatures that alter the expression of specific gene clusters in selected Bacteroidota and Bacillota species. Both the phyla possess variable and sophisticated loci of mannan sensing proteins, hydrolytic enzymes, transporters, and other metabolic proteins to sense, capture and utilize mannans as an energy source. The current review summarizes mannan structural diversity, and strategies opted by select bacterial species of the HGM to forage mannans by focusing primarily on glycoside hydrolases and their effects on host health and metabolism
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