109 research outputs found
A proposed increase in retinal field-of-view may lead to spatial shifts in images Doshi R, Day PJR.
No full textA proposed increase in retinal field-of-view may lead to spatial shifts in images Doshi R, Day PJR.
No full textA proposed increase in retinal field-of-view may lead to spatial shifts in images Doshi R, Day PJR.
No full textNumerical and experimental study of a droplet-based PCR chip
No full textA two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface. Samples contained in aqueous droplets are continuously moved by an oil carrier-fluid through various temperature zones, introducing the possibility of real-time quantitative PCR. In the present paper, we investigate many of the factors affecting droplet-based PCR chip design, including thermal mass, flow rate, and thermal resistance. The study focuses particularly on the fluid and substrate temperature distribution within the PCR chip and the droplet residence times in critical temperature zones. The simulations demonstrate that the flow rate strongly affects the temperature field within the carrier-fluid. Above a critical flow rate, the carrier-fluid fails to achieve the required temperatures for DNA amplification. In addition, the thermal resistances of the different layers in the chip are shown to have a major impact on the temperature profile in the channel
The specific detection of bacterial pathogens using oligonucleotide microarrays generated from hydrolysis PCR probe sequences.
No full text
Specific detection of bacterial pathogens using oligonucleotide microarrays generated from hydrolysis PCR probe sequences.
No full textA move away from high-density screening array formats and the implementation of probes specifically identifying targets for application in low-density hybridization capture analyses is growing in importance. Some of the highest specificity for bioassays that encompasses use of hybridization has enlisted hydrolysis probes in real-time PCR. It is demonstrated that by employing 5'-end-tethering to glass of hydrolysis PCR probe sequences that these can be employed as capture probe sequences. The probes retain hybridization binding specificity to their PCR amplicons and specificity of hybridization was achieved across all probes at equivalent stringency. This suggests that probes identified for use in hydrolysis PCR using one set of temperature cycling parameter can also be applied to a microarray, where again the probes all exhibit similar specificity of interact at analogous hybridization conditions. The procedure permits the exact same PCR amplicon sequences to be used in both quantitative PCR and qualitative microarray assay formats
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