9,602 research outputs found

    Ethanol production and maximum cell growth are highly correlated with membrane lipid composition during fermentation as determined by lipidomic analysis of 22 saccharomyces cerevisiae strains

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    Optimizing ethanol yield during fermentation is important for efficient production of fuel alcohol, as well as wine and other alcoholic beverages. However, increasing ethanol concentrations during fermentation can create problems that result in arrested or sluggish sugar-to-ethanol conversion. The fundamental cellular basis for these problem fermentations, however, is not well understood. Small-scale fermentations were performed in a synthetic grape must using 22 industrial Saccharomyces cerevisiae strains (primarily wine strains) with various degrees of ethanol tolerance to assess the correlation between lipid composition and fermentation kinetic parameters. Lipids were extracted at several fermentation time points representing different growth phases of the yeast to quantitatively analyze phospholipids and ergosterol utilizing atmospheric pressure ionization-mass spectrometry methods. Lipid profiling of individual fermentations indicated that yeast lipid class profiles do not shift dramatically in composition over the course of fermentation. Multivariate statistical analysis of the data was performed using partial least-squares linear regression modeling to correlate lipid composition data with fermentation kinetic data. The results indicate a strong correlation (R² = 0.91) between the overall lipid composition and the final ethanol concentration (wt/wt), an indicator of strain ethanol tolerance. One potential component of ethanol tolerance, the maximum yeast cell concentration, was also found to be a strong function of lipid composition (R² = 0.97). Specifically, strains unable to complete fermentation were associated with high phosphatidylinositol levels early in fermentation. Yeast strains that achieved the highest cell densities and ethanol concentrations were positively correlated with phosphatidylcholine species similar to those known to decrease the perturbing effects of ethanol in model membrane systems.Clark M. Henderson, Michelle Lozada-Contreras, Vladimir Jiranek, Marjorie L. Longo, David E. Bloc

    Finding efficient distinguishers for cryptographic mappings, with an application to the block cipher TEA

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    Congress on Evolutionary Computation. 8-12 December 2003A simple way of creating new and efficient distinguishers for cryptographic primitives such as block ciphers or hash functions is introduced. This technique is then successfully applied over reduced round versions of the block cipher TEA, which is proven to be weak with less than five rounds

    Teoria das Situações Didáticas e Engenharia Didática

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    Ponencia presentada en el 1º Simposio Latinoamericano de Didáctica de la Matemática. Bonito (Brasil), 1 al 6 de noviembre de 2016.Fil: Fregona, Dilma. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina.Fil: Block, David. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados; México.Fil: Orús, Pilar. Universitat Jaume I; EspañaEl taller, asociado a la conferencia "A Engenharia Didática entre Pesquisa e Recurso para o Ensino e a Formação de Professores" dictada por Marie-Jeanne Perrin-Glorian (Universidade de Artois/França) e Paula Baltar (UFPE/PE-Brasil), analiza desde la óptica de la teoría de las situaciones didácticas, las decisiones didácticas tomadas en una secuencia de enseñanza de la división y de la multiplicación en N. El documento tomado es una publicación del IREM de Bordeaux (BROUSSEAU, N. et al, 1985), elaborado por docentes de la Escuela Michelet e investigadores en didáctica de la matemática.http://ladima.tuseon.com.br/anais---conferencias-e-oficinas.htmlsubmittedVersionFil: Fregona, Dilma. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina.Fil: Block, David. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados; México.Fil: Orús, Pilar. Universitat Jaume I; EspañaEducación General (incluye capacitación, pedagogía y didáctica

    D-0173: 533 West 100 South, Logan, Utah, David E. Daley residence. Lot 1 Block 8 Plat A. Built 1924

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    D-0173: 533 West 100 South, Logan, Utah, David E. Daley residence. Lot 1 Block 8 Plat A. Built 192

    E-1267: Mendon, Utah, David Buist/Geraldine Krambule residence. Lot 1 Block 37 Plat A. Built 1899

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    E-1267: Mendon, Utah, David Buist/Geraldine Krambule residence. Lot 1 Block 37 Plat A. Built 1899 (2 photos

    A Reading of the David and Goliath Narrative in Greek and Hebrew

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    The story of David and Goliath existed in antiquity in two distinct literary versions, a short version found in LXXB and a longer version reflected in the MT. This thesis proposes that each version is worthy of study in its own right and offers a close literary reading of the narrative of David and Goliath in the Greek text of 1 Reigns 16-18. In this study we explore a method of reading the Septuagint that recognizes it is both a document in its own right and a translation of a Hebrew original. In offering this reading of the septuagintal version of the David and Goliath narrative we will highlight the literary difference between the two final versions of the story that exist in LXXB and MT

    Residential water demand under block rates: a Portuguese case study

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    A residential water demand equation is estimated using a panel data sample of 5 Portuguese local communities and 72 months, corresponding to a total number of 360 observations. Because of the presence of multi-part tariffs, we use as explanatory variables the two common price-related variables: marginal price and difference. To prevent the simultaneity bias from using observed quantities to determine the values of marginal price and difference directly from the rate schedule, we use an instrumental variable approach to create a constant marginal price and difference parameters for each rate structure. The price elasticity value obtained fall within the range of those found in other case studies. Thus, although presenting weak elasticity, price seems to play a role in water demand management. However, we do not confirm the expected influence of difference on residential water demand. This can be a consequence of the complexity of the Portuguese water tariffs and the confusing signs that come from the simultaneous use of fixed quotas and increasing block tariffs. So, it is imperative to clarify water tariffs objectives by reviewing the Portuguese water tariffs design processes.demand, water utilities, pricing policy.

    The comparing of ultrasound-guided techniques:sciatic block with continous lumbar plexus block or continous femoral nerve block for aneshtesia and analgesia of total knee replacement

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    Abstract: Background and Aims: This double blind prospective randomized clinical trial evaluated the efficacy and safety of continuous ultrasound-guided lumbar plexus block compared to continuous ultrasound-guided femoral nerve block, in the intra-operative and postoperative periods after total knee replacement. Methods: Forty ASA I-III patients were randomized to receive: continuous femoral block (n= 20, 30 ml of ropivacaine 5 mg/ml) or continuous lumbar plexus block (n= 20, 30 ml of ropivacaine 5 mg/ml) both in association with single injection sciatic nerve block. All patients received continuous infusion of 2 mg/ml of ropivacaine at 8 ml/h for 48 hours and intra- venous morphine for patient-controlled analgesia. Primary outcomes were intra-operative sufentanil consumption and verbal analogue scale (VAS) score at rest at 24h follow up. Results: Intra-operative sufentanil consumption was higher in the femoral block (FEM) group compared to the lumbar plexus block (PSOAS) group (FEM: 10.00 (10.00, 17.50) μg; PSOAS: 2.50 (0.00, 10.00) μg. p= 0.002). Obturator motor blockade occurred more frequently in the PSOAS group (70%) than in the FEM group (40%) (p=0.1); however, we found no differences in sensory blockade (p=0.6). VAS at rest was similar in the two groups at 24h postoperatively (FEM: 29.50 ± 14.74 mm; PSOAS: 25.60 ±17.42 mm. p=0.4), and throughout the follow-up period. No differences were detected in pain scores during physiotherapy. Conclusion: Continuous femoral and lumbar plexus blocks, both in association with sciatic nerve block, provided similar VAS scores at 24h, and throughout the follow-up period; intra-operative sufentanil consumption was, however, lower in the lumbar plexus block group

    An Approximate Conflict Detection and Resolution Model for Moving-Block Signalling by Enhancing RECIFE-MILP

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    Conflict detection and resolution models are being developed to support railway traffic management in taking optimised rescheduling decisions in case of disturbances. Existing models mostly concern fixed-block signalling systems, in which minimum train separation distances are determined based on a preset number of blocks representing worst-case braking distances. In a moving-block signalling system, minimum train separation is based on absolute braking distances and hence depends on train speed differently from how fixed-block conflict detection and resolution models. In this paper, we propose a conflict detection and resolution model that approximates moving-block operations. The model enhances the state-of-the-art fixed-block rescheduling model RECIFE-MILP. The enhancements include a reconsideration of the discretisation of the infrastructure, the introduction of a speed profile alternative and a redefinition of the blocking times. We verify the model by comparing the solutions of the moving-block version with the fixed-block version for a specific scenario. The results indicate that the moving-block model can propose different rescheduling decisions than the fixed-block model with a better delay recovery.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Transport and Plannin

    D-2094: 546 North 600 East, Logan, Utah, David S. Jennings residence. Lot 4 Block 2 Plat E

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    D-2094: 546 North 600 East, Logan, Utah, David S. Jennings residence. Lot 4 Block 2 Plat
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