415 research outputs found
Calvin Carter, Ronald Craven, and David Beebe Working at O.H. Carter Company Incorporated, C
No full textCalvin Carter (left), Ronald Craven (right), and David Beebe (middle) works in a conference room at the insurance company, O.H. Carter Co. Inc. which was founded in 1927, in Tampa, Florida.https://digitalcommons.usf.edu/gandy_commercial/3426/thumbnail.jp
Activation of the Jak–STAT-Signaling Pathway in Embryonic Lens Cells
No full textAbstractPrevious studies showed that lens epithelial cells proliferate rapidly in the embryo and that a lens mitogen, most likely derived from the blood, is present in the anterior chamber of the embryonic eye (Hyatt, G. A., and Beebe, D. C.,Development117, 701–709, 1993). Messenger RNAs for several growth factor receptors have been identified in embryonic lens epithelial cells. We tested several growth factors that are ligands for these receptors for their ability to maintain lens cell proliferation. Embryo serum, PDGF, GM-CSF, and G-CSF maintained lens cell proliferation, but NGF, VEGF, and HGF did not. This and a previous study (Potts, J. D., Harocopos, G. J., and Beebe, D. C.,Curr. Eye Res.12, 759–763, 1993) detected members of the Janus kinase family (Jaks) in the developing lens. Because Jaks are central players in the Jak-STAT-signaling pathway, we identified STAT proteins in the lens and tested whether they were phosphorylated in response to mitogens. STAT1 and STAT3, but not STAT 5 were detected in chicken embryo lens epithelial cells. Only STAT3 was found in terminally differentiated lens fiber cells. STAT1 and STAT3 were phosphorylated in lens cells analyzed immediately after removal from the embryo and when lens epithelial explants were treated with embryo serum, PDGF, or GM-CSF, but not with NGF. Chicken embryo vitreous humor or IGF-1, factors that stimulate lens cell differentiation, but not proliferation, did not cause STAT phosphorylation. When lens epithelial cells were cultured for 4 h in unsupplemented medium, STAT1 and STAT3 declined to nearly undetectable levels. Treatment with PDGF or embryo serum for an additional 15 min restored STAT1 and -3 levels. This recovery was blocked by cycloheximide, but not actinomycin D, suggesting that STAT levels are regulated at the level of translation. STAT levels were maintained in epithelial explants by lens mitogens, but not by factors that stimulated lens fiber differentiation. Both factors that stimulated lens cell proliferation and those that caused fiber differentiation protected cultured lens epithelial cells from apoptosis. These data suggest that the factor(s) responsible for lens cell proliferationin vivoactivates the Jak–STAT-signaling pathway. They also indicate that growth factors maintain STAT protein levels in lens epithelial cells by promoting the translation of STAT mRNA, an aspect of STAT regulation that has not been described previously. Signaling by most of the growth factors and cytokines known to activate the Jak-STAT pathway has been disrupted in mice by mutation or targeted deletion. Consideration of the phenotypes of these mice suggests that the factor responsible for lens cell proliferationin vivomay be a growth factor or cytokine that has not yet been described
Localization of Smad4 (A), Smad7 (B),) and Smad6 (C), c-Ski (D) and TGIF (E) in lens epithelial (LE) and fiber cells (LF)
No full text<p><b>Copyright information:</b></p><p>Taken from "Intracellular mediators of transforming growth factor β superfamily signaling localize to endosomes in chicken embryo and mouse lenses in vivo"</p><p>http://www.biomedcentral.com/1471-2121/8/25</p><p>BMC Cell Biology 2007;8():25-25.</p><p>Published online 25 Jun 2007</p><p>PMCID:PMC1914053.</p><p></p> Nuclear localization of Smad4 and TGIF is seen mainly in lens fiber cells. Smad7 is seen in the nuclei of both epithelial and fiber cells, although staining is stronger in fiber cell nuclei. Both I-Smads, Smad7 and Smad6, are abundant in the cytoplasm of lens epithelial cells. c-Ski is found mainly to the cytoplasm. A-C are sections of E7 chicken lenses and D-E are P3 mouse lenses
Scanning tunneling microscopy imaging of uncoated biological material
No full textPT: J; CR: AMREIN M, 1989, SCIENCE, V243, P1708 BEEBE TP, 1989, SCIENCE, V243, P370 BINNIG G, 1986, REV SCI INSTRUM, V57, P1688 BLACKFORD BL, 1987, REV SCI INSTRUM, V58, P1343 BLACKFORD BL, 1988, J MICROSC-OXFORD, V152, P237 BLACKFORD BL, 1989, ULTRAMICROSCOPY, V26, P427 DAHN DC, 1988, J VAC SCI TECHNOL A, V6, P548 JAMES MNG, 1986, NATURE, V319, P33 JERICHO MH, 1987, REV SCI INSTRUM, V58, P1349 JERICHO MH, 1989, J APPL PHYS, V65, P5237 LINDSAY SM, 1989, SCIENCE, V244, P1063 MAMIN HJ, 1986, PHYS REV B, V34, P9015 MICHAEL NG, 1985, BIOCHEMISTRY-US, V24, P3701 NATALIA S, 1984, J BIOCHEM-TOKYO, V259, P11353 STEWART M, 1985, J MOL BIOL, V183, P509; NR: 15; TC: 25; J9: J VAC SCI TECHNOL A; PG: 6; GA: CL594Source type: Electronic(1
American Geographical Society of New York Records, 1723-2010, bulk 1854-2000
No full textIncludes correspondence between Charles P. Daly and the following individuals and organizations: Theodore Baab (New York Furniture Board of Trade), Charles Baird (The Manse, Rye, NY), Frederick Augustus Porter Barnard (Columbia College, articles on polar regions), John Banvard, Emily Weed Barnes, John R. Bartlett (“The Blake”, U.S. Coast and Geodetic Survey), Arthur Beckwith, Adeline Beebe (widow of Welcome R. Beebe), P.O. Beirne, Clark Bell (early Dutch voyages), H.W. Bellows, Erastus C. Benedict (Law Offices of Benedict, Taft, & Benedict), J. Bennett, J.A. Bennett, Silas Bent, Norman S. Bentley (Oregon Pacific Railroad), Theodore? Berendsohy, A.R.? Berns, Albert S. Bickmore (American Museum of Natural History), Henri Bionne, George William Blunt (Board of Commissioners of Pilots), Franz Boas (American Museum of Natural History), David P. Bolton, J.? Bonton, John W. Booth (USS Polaris), D.N. Botassi (Consul-General of Greece), Elias Cornelius Boudinot, W. Bradford (Century Club), J. Carson Brevoort, Sidney Brooks, A.G. Browne, Jr., Captain E.? Bruno, Albert Bushnell, and James D. Butler (Wisconsin State Historical Society)
Endosomal localization of pSmad1, pSmad2, TGIF, C184M and c-Ski in P3 mouse lenses
No full text<p><b>Copyright information:</b></p><p>Taken from "Intracellular mediators of transforming growth factor β superfamily signaling localize to endosomes in chicken embryo and mouse lenses in vivo"</p><p>http://www.biomedcentral.com/1471-2121/8/25</p><p>BMC Cell Biology 2007;8():25-25.</p><p>Published online 25 Jun 2007</p><p>PMCID:PMC1914053.</p><p></p> A. Rab5B (red), pSmad1 (green). B. EEA1 (green), pSmad2 (red). C. EEA1 (green), TGIF (red). D. EEA1 (green), C184M (red). E. c-Ski (green), C184M (red). F is a diagram of the neonatal mouse lens showing the regions that are represented in each of the images
Spinal anesthesia instead of general anesthesia for infants undergoing tendon Achilles lengthening
No full textMohammad AlSuhebani,1 David P Martin,1,2 Lance M Relland,1,2 Tarun Bhalla,1,2 Allan C Beebe,3 Amanda T Whitaker,3 Walter Samora,3 Joseph D Tobias1,2 1Department of Anesthesiology & Pain Medicine, Nationwide Children’s Hospital, Columbus, OH, USA; 2Department of Anesthesiology & Pain Medicine, The Ohio State University College of Medicine, Columbus, OH, USA; 3Department of Orthopedic Surgery, Nationwide Children’s Hospital and The Ohio State University College of Medicine, Columbus, OH, USA Abstract: Spinal anesthesia (SA) has been used relatively sparingly in the pediatric population, as it is typically reserved for patients in whom the perceived risk of general anesthesia is high due to comorbid conditions. Recently, concern has been expressed regarding the potential long-term neurocognitive effects of general anesthesia during the early stages of life. In view of this, our center has developed a program in which SA may be used as the sole agent for applicable surgical procedures. While this approach in children is commonly used for urologic or abdominal surgical procedures, there have been a limited number of reports of its use for orthopedic procedures in this population. We present the use of SA for 6 infants undergoing tendon Achilles lengthening, review the use of SA in orthopedic surgery, describe our protocols and dosing regimens, and discuss the potential adverse effects related to this technique. Keywords: spinal anesthesia, orthopedic surgery, tendon Achilles lengthenin
A microanalytic study of gaze and expressivity in at risk dyads: Psychotic mothers and their infants at three months of age
No full textMaternal psychosis is an important risk factor for infant’s development, especially when maternal pathology arises before pregnancy during the first three years of infant’s life. Some observational studies of early interaction between psychotic mothers and their infants outlined the quality of maternal interaction, pointing out a lower level of maternal stimulation toward social stimuli, and slower and inadequate responding to infant’s signals, (Seifer & Dickstein, 2000, Lamour, 1989; David, 1987) compared to mothers without psychiatric symptoms. Moreover, psychotic mothers appear more hesitant and rigid during the interaction with their infants, and show tendencies to smile and talk less. Maternal behaviours seem coupled with some defensive behaviour in the infant, shown by gaze aversion or ipervigilant gaze (Fraiberg, 1982; Beebe, 2004).
AIMS OF THE STUDY
Our study aims to examine the communicative micro-processes of 5 psychotic mothers and their infants at 3 months of life, a period characterized in non-pathological dyads by a high level of exchange and emotional intensity during face to face interaction. In particular:
To examine the pattern of associations between infant’s gaze direction and maternal timing in the course of interaction;
To examine the co-occurrence of infant and maternal emotional expressions;
To examine the sequential structure of the interaction, in order to have a clearer image of the misregulated episodes, for addressing clinical interventions.
PARTICIPANTS
5 mothers with a diagnosis of schizophrenic psychosis and their 3 months old infants were recruited among the patients of the Psychiatric Unit of Wiesloch Psychiatric Hospital. The participants were observed under different conditions, but in this study only the face-to-face interaction will be considered
PROCEDURE
Mothers are requested to play freely for 5 minutes in a standardized setting into a play room in the Hospital, where also some little toys were available. The interaction was videotaped with two cameras and decoded by two trained observers of Bologna University, who were unaware of the psychiatric syntoms of the mothers.
An event-based approach with temporal information was adopted to decode the sequences of five minutes interaction. The behavioural dimensions considered in this study were: infant’s gaze direction, facial expressions for both partners, maternal timing .
RESULTS
These data support the evidences reported in literature (Beebe, 2004; Beebe & Stern, 1977), showing a high level of gaze aversion in infants when associated to inadequate timing of maternal behaviour. Compared to at norm dyads, these results highlight disorganized gaze behaviour and a low level of positive emotions in the mutual exchanges between the mother and the infant; surprisingly, the child shows a more “depressed” emotional pattern, and a intense use of visual cut-off to signal discomfort. Gaze orientation, so, seems to be a very crucial issue to be considered both in research and in clinical intervention
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