2,882 research outputs found
Determining the Location of the Alpha-Synuclein Dimer Interface using Native Top-Down Fragmentation and Isotope Depletion-Mass Spectrometry
No full textAlpha-synuclein (αSyn), a 140-residue intrinsically disordered protein, comprises the primary proteinaceous component of pathology-associated Lewy body inclusions in Parkinson’s disease (PD). Due to its association with PD αSyn is studied extensively; however the endogenous structure and physiological roles of this protein are yet to be fully understood. Here, ion mobility-mass spectrometry and native top-down electron capture dissociation fragmentation have been used to elucidate the structural properties associated with a stable, naturally-occurring dimeric species of αSyn. This stable dimer appears in both wild-type (WT) αSyn and the PD-associated variant A53E. Furthermore, we integrated a novel method for generating isotopically depleted protein into our native top-down workflow. Isotope depletion increases signal-to-noise ratio and reduces the spectral complexity of fragmentation data, enabling the monoisotopic peak of low abundant fragment ions to be observed. This enables the accurate and confident assignment of fragments unique to the αSyn dimer to be assigned, and structural information about this species to be inferred. Using this approach, we were able to identify fragments unique to the dimer, which demonstrates a C-terminal to C-terminal interaction between the monomer subunits. The approach in this study holds promise for further investigation into the structural properties of endogenous multimeric species of αSyn. The data relates to the following publication:
Kiani Jeacock, Alexandre Chappard, Kelly J. Gallagher, C. Logan Mackay, David P. A. Kilgour, Mathew H. Horrocks, Tilo Kunath and David J. Clarke. 'Determining the Location of the Alpha-Synuclein Dimer Interface using Native Top-Down Fragmentation and Isotope Depletion-Mass Spectrometry.' J. Am. Soc Mass Spec. (in submission)
Figs. 121–124 in A Revision of the New Zealand Endemic Rove Beetle Genus Agnosthaetus Bernhauer (Coleoptera: Staphylinidae)
No full textFigs. 121–124. Aedeagi of species in the Agnosthaetus brouni species-group, dorsal (left) and right lateral (right) views. 121) A. brouni; 122) A. tumidus; 123) A. aequalis; 124) A. akatarawa. Scale bar = 100 mm.Published as part of Clarke, Dave J., 2011, A Revision of the New Zealand Endemic Rove Beetle Genus Agnosthaetus Bernhauer (Coleoptera: Staphylinidae), pp. 1-118 in The Coleopterists Bulletin (mo10) 2011 on page 94, DOI: 10.1649/0010-065X-65.mo4.1, http://zenodo.org/record/490708
Replacement Names for <i>Elwoodius</i> Clarke & Oberprieler and <i>Platychirus</i> Clarke & Oberprieler (Coleoptera: Curculionoidea: Mesophyletidae)
No full textIn a recent paper we published on the weevil fauna preserved in Burmese amber, two newly proposed generic names were subsequently identified as preoccupied names (Elwoodius Clarke & Oberprieler and Platychirus Clarke & Oberprieler). We propose the name Zimmiorhinus as a replacement name for Elwoodius Clarke & Oberprieler and Burmophyletis as a replacement name for Platychirus Clarke & Oberprieler
Exploring small area demand for grocery retailers in tourist areas
No full textNewing, A., Clarke, G.P. and Clarke, M. 2014. Exploring small area demand for grocery retailers in tourist areas. Tourism Economics, 20(2), pp.407-427This paper uses data from a major loyalty card scheme to draw insights about the characteristics of grocery expenditure by tourists. The authors explore the volume, value and composition of store based visitor expenditure using consumer data from the loyalty card scheme. They focus on grocery spending at selected stores in Cornwall, a popular tourist destination in South West England. Theloyalty card data provide a valuable source rarely available for academic investigations. The authors are able to analyse visitor spend by socio-economic and geodemographic characteristics, drawing a range of comparisons with residential demand from within the store catchment areas. They demonstrate that visitor grocery expenditure is complex and varies by store, destination and type of customer. The paper presents evidence to suggest that the current approaches used to estimate sales uplift and local-level economic impact from visitor demand are unable to account for the complexities of this form of expenditure. Based on these insights, the authors recommend that sophisticated modelling is employed to estimate the impact of visitor expenditure
Agnosthaetus leviceps Clarke 2011, new species
No full text(17) Agnosthaetus leviceps Clarke, new species (Figs. 137–139, Map 4) Type Material. Holotype. ♂, with four labels: " New Zealand SL| 2km SW Longwood | Trig [46°13.602′S, 167°50.292′E] 610m | Longwood Ra| 1 Feb 1976 / L.L. Deitz | moss in forest| 76/11/ FMNH-INS 0000 038 382 / HOLOTYPE Agnosthaetus leviceps Clarke, ♂, design. D. Clarke 2011", in NZAC. Paratypes. 2 specimens (2♂). Same data as holotype, 2♂, FMNH-INS 38380–381 (in NZAC). Diagnosis. Agnosthaetus leviceps may be distinguished from all other known Agnosthaetus species by the combination of lack of punctures on the dorsum of the head (cf. A. nitidus, with indistinct punctures), medial pronotal sulcus separated from both anterior setal puncture and basal fovea (Fig. 24, ap, bf; similar to A. vicinus: Fig. 75), incomplete to largely effaced lateral pronotal carina (similar to Fig. 80, pc), and absence of an elytral sutural stria. The aedeagus differs from all others with aedeagus " Type A" in having copious, long apicomedial setae on the median lobe (Fig. 139). Description. Color: Attractive, pale brown, and shiny species. Head: Frontal ridge absent. Dorsum impunctate on disc; with distinct shallow punctures only posterolaterally. Dorsal microsculpture absent. Dorsal tentorial sulcus (cf. Figs. 10–11, dt) broadly ovate. Sublongitudinal ridge (cf. Fig. 10, sr) distinct; not confused by smaller carinae or punctures; crest at antennal tubercle without distinct microsculpture. Area above and behind antennoocular carina (Figs. 10–11, arrow) with distinct subsidiary carina formed by confluent punctures. Antenno-ocular carina joining eye behind middle (cf. Fig. 59). Temple (Fig. 11, tm) long, greater than 50% EYL. Subocular surface without microsculpture, completely smooth between punctures (cf. Figs. 63–64). Apical labral margin in males moderately broadly and shallowly emarginate medially, evenly dentate, with 16–18 teeth (n =3), all teeth normal, projecting more or less anteriorly (Fig. 137). Adoral labral surface in males smooth, without subapical transverse ridge. Mandible more or less identical in both sexes; males with single, mesially directed tooth, without preapical spur (cf. Fig. 189); females with single, mesially projecting tooth, without spur. Prothorax: Pronotum without microsculpture. Medial pronotal sulci anteriorly separate from, and terminating posterior to anterior punctures; basally distant from remnant basal foveae (cf. Fig. 75). Distance between medial sulci slightly greater posteriorly. Lateral pronotal sulcus partly to completely interrupted at middle (cf. Figs. 77, 80, ls). Pronotal basolateral carina present, but only weakly developed. Base of pronotum without pair of median foveae (cf. Fig. 75); without lateral foveae, or with single distinct lateral fovea. Pronotal macrosetal punctures absent (cf. Fig. 77). Medial pronotal seta adjacent to lateral sulcus (cf. Fig. 77). Lateral pronotal carina, anteriorly complete, continuous with anterior pronotal margin and incomplete, usually more or less completely absent beyond middle of pronotum, if present anteriorly then difficult to discern even at high magnification (cf. Fig. 80, pc). Pronotal hypomeron (Fig. 24, hy) shiny, without microsculpture. Prosternum (Fig. 24, pro) impunctate; with distinctly reticulate microsculpture. Pterothorax: Elytron (Fig. 23, e) without microsculpture; without sutural stria; without macrosetae; laterally with single ridge (cf. Fig. 24, ek). Mesothoracic epimeral region (Fig. 24, mer) shiny, without microsculpture. Metathoracic pleural region (Fig. 24, m) shiny, without microsculpture. Metathoracic pleural ridge absent (cf. Fig. 86); metathoracic pleural groove (Fig. 24, gr) complete, continuing to or near to pleurocoxal articulation. Abdomen: Abdominal vestiture short, somewhat appressed, dorsally more or less evenly projecting posteriorly. Abdominal sternite VI of male with setose apicomedial protuberance. Aedeagus (Fig. 138): " Type A" (see description on p. 8). Apical part of median lobe with rounded lateral lobes and abruptly distinct, broadly acuminate, sharply pointed apicomedian lobe. Apicolateral setae small; apicomedial setae up to 10X longer than apicolateral setae (Fig. 139). Paramere extending to about level of median lobe apex; in lateral view produced apically into lobe; in dorsal view with outer side gently convex; with 4 large setae at apex. Etymology. The specific epithet leviceps is a noun in apposition from the Latin levis (smooth) combined with ceps (head) in reference to the unique lack of punctation on the dorsum of the head. Distribution. (Map 4). South Island: SL. Remarks. The holotype and paratypes of this species share some unusual and otherwise unique pronotal characters with A. nitidus and A. vicinus. From both of these species, A. leviceps can be distinguished most easily by the lack of the elytral sutural stria. The male secondary sexual character on abdominal sternite VI is a unique structure within Agnosthaetus.Published as part of Clarke, Dave J., 2011, A Revision of the New Zealand Endemic Rove Beetle Genus Agnosthaetus Bernhauer (Coleoptera: Staphylinidae), pp. 1-118 in The Coleopterists Bulletin (mo 10) (mo 10) 2011 on page 47, DOI: 10.1649/0010-065X-65.mo4.1, http://zenodo.org/record/490708
Creophilus Leach 1819
No full textTHE <i>CREOPHILUS</i> COMPLEX <p> <i>Adult diagnosis:</i> Species of the <i>Creophilus</i> complex can be recognized by the combination of: disc of head and pronotum glabrous; ventroscapal macrosetae absent or reduced; superior marginal pronotal carina separate from inferior marginal carina along entire length, effaced apically (Fig. 2E, <i>il</i>, <i>sl</i>, Fig. 9J, right and middle arrows); inferior marginal pronotal carina continuous with anterior marginal carina (Fig. 9J, left and middle arrows); mesepimeron with single marginal ridge (Fig. 9K, arrow); male sternite IX subtruncate to shallowly emarginate apically, produced into elongate process basally (Fig. 3Q); median lobe of aedeagus with large apicolateral sclerites (Fig. 3, <i>as</i>); internal sac without ventral basomedian sclerotized strip (as in Fig. 3K, <i>bms</i>).</p> <p> <i>Remarks:</i> The following taxa are here included in the <i>Creophilus</i> complex: <i>Creophilus</i> (12 species), <i>Hadrotes crassus</i>, <i>Ht. wakefieldi</i>, <i>Hadropinus fosso</i> r, <i>Liusus</i> (two species), <i>Thinopinus</i> pictus.</p>Published as part of <i>Clarke, Dave J., 2011, Testing the phylogenetic utility of morphological character systems, with a revision of Creophilus Leach (Coleoptera: Staphylinidae), pp. 723-812 in Zoological Journal of the Linnean Society (Zool. J. Linn. Soc.) (Zool. J. Linn. Soc.) 163 (3)</i> on page 755, DOI: 10.1111/j.1096-3642.2011.00725.x, <a href="http://zenodo.org/record/5441985">http://zenodo.org/record/5441985</a>
Creophilus maxillosus
No full text<i>CREOPHILUS MAXILLOSUS</i> SPECIES- GROUP <p>This species group was originally proposed by Newton (1985) for the Holarctic and Oriental species. With recognition of three new synonyms and description of one new species, five species are included here.</p> <p> <i>Diagnosis:</i> Head black or brownish-black; antennomere 2 subequal in length to 3 or shorter; antennomere 11 with apical pairs of setae situated on either side of antennal axis (e.g. Fig. 13F); right mandible with T3 directly ventral to T2 and mandibular blade, mostly (in <i>C. incanus</i> entirely) hidden from dorsal view beneath mandibular blade (Fig. 13A); anterior pronotal angles produced laterally (Figs 2G, 13E); abdominal tergites III–V without secondary transverse basal ridge; apical fringe of tergites III–VI with at least paired groups of white or golden setae medially, rest of body with variably black, brown, whitish-grey, or golden-brown vestiture (Fig. 1F–J); median lobe of aedeagus completely divided ventrally by membranous strip (Fig. 3C, arrow). Large males with: apical portion of mandibles exceeding 0.9 times length of basal portion (ML1/ML2 range of all males = 0.60–1.09); without ventrolateral carina of head; pronotum widest at anterior angles; base of profemur with spine-like apophysis (Fig. 9D, arrow); superior pronotal marginal line abruptly deflected beneath disc of pronotum at point of basolateral denticle (Fig. 2G, <i>bpd</i>).</p>Published as part of <i>Clarke, Dave J., 2011, Testing the phylogenetic utility of morphological character systems, with a revision of Creophilus Leach (Coleoptera: Staphylinidae), pp. 723-812 in Zoological Journal of the Linnean Society (Zool. J. Linn. Soc.) (Zool. J. Linn. Soc.) 163 (3)</i> on page 759, DOI: 10.1111/j.1096-3642.2011.00725.x, <a href="http://zenodo.org/record/5441985">http://zenodo.org/record/5441985</a>
A Quasi-2D Bayesian network model for assessments of coastal inundation pathways and probabilities
No full textCoastal flood assessments often require the analysis of a complex system of flood sources, pathways and receptors. This can be challenging for traditional numerical modelling approaches. In this paper we use a Bayesian networks approach to assess coastal floodplains as networks of inter-linked elements. A Bayesian network (Bn) model is built to describe flood pathways and estimate flood extents for different extreme events. The network of the Bn model is constructed from a quasi-2D Source – Pathway – Receptor (SPR) systems diagram. The Bn model is applied in Teignmouth in the UK, a coastal floodplain of typical complexity. It identifies two key flood pathways and assesses their sensitivity to changes in sea levels, beach widths and coastal defences. The advantages, utility and limitations of the Teignmouth Bn model are discussed. The process of 2D SPR and Bn model construction helps identify gaps in floodplain understanding and description. The Bn model quantifies inundation probabilities and facilitates the rapid identification of critical pathways and elements, before committing resources to further detailed analysis. The approach is transferable and can be readily applied in local-scale coastal floodplains to obtain a systems-level understanding and inform numerical modelling assumptions
Odontocroton apicalis Clarke 2018, n. comb.
No full textOdontocroton apicalis (Klug, 1825), n. comb. (Fig. 15–18) Stenopterus apicalis Klug 1825: 472. Odontocera apicalis White 1855: 188; Monné 2017: 440 (cat.). Species concept. Based on Klug (1825) original description and figure, and photograph (Fig. 15-16) of the female holotype kindly sent to the author by Joachim Willers of the Humboldt Museum, which compares reasonably well, given the variation in color of this species, with the male specimen (Fig. 17-18) identified by J. Melzer, kindly lent to the author by the MZSP. Measurements (mm). 1 male: total length, 16.10; length of prothorax, 2.60; width of prothorax, 2.10; length of elytra, 9.75; width at humeri, 2.60. Specimen analyzed. BRAZIL, Minas Gerais, Passa Quatro, Faz. dos Campos, male, 28.I.1920, J.F. Zikán leg. (MZSP). Distribution. Klug stated that his specimen came from Brazil. The author believes the distribution of this species to be centered on Brazil (MG), where Zikán collected his specimen; and records from southern Brazil, Argentina and Uruguay require verification.Published as part of Clarke, Robin O. S., 2018, Provisional revision of the genus Odontocera Audinet-Serville, 1834 (Coleoptera: Cerambycidae). I: exclusions, new rank, synonymies and the description of two new genera, pp. 1-27 in Insecta Mundi 637 on page 12, DOI: 10.5281/zenodo.370813
Globalising care? Town twinning in Britain since 1945
No full textTown twinning describes the establishment and practice, by various groups and to various ends, of relatively formal and long-term relationships between settlements usually located in different nation-states. Twin towns are sometimes called sister cities. This paper draws on a study of town twinning that focused on the involvement of British localities since the end of the Second World War and analysed data collected by the Local Government Association (for England and Wales), materials archived in the National Archives at Kew, London and various local record offices, and transcripts of interviews with representatives of relevant local, national, and international organisations. The paper makes three main contributions. Firstly, it provides a brief history of town twinning involving British localities. Secondly, it develops from this historical narrative an original conceptualisation of town twinning, arguing that it should be approached less as a coherent movement and more as a device, a repertoire, and a model. Thirdly, it argues that town twinning has often been used as a device for extending care across space – and that much can be learned from its history for contemporary geographies of care. Town twinning participants have approached the problem of care-at-a-distance as both an ontological problem and a practical problem. Some have focused more than others on the role of distanciated causal relationships in the generation of needs in distant places. Some are currently encountering another problem as they attempt to globalise care: the problem of care-in-a-hurry
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