2 research outputs found
Nivel de infestación y asociación ecológica de Nosema spp. (Microsporida: Nosematidae) y Varroa destructor (Mesostigmata: Varroidae) en colonias de abejas del suroeste de Nigeria
No full textThe level of infestation and ecological association of Nosema spp. and Varroa destructor was studied in honeybees. The infestation rate (Mn±SD) of V. destructor ranged from 1.05 ± 0.64% to 9.02 ± 4.98%. The prevalence of Nosema spp. in Osun State was found to range from low to semi-severe, while in Ogun state, infestation leves varies from moderate to severe. However, in both states severe infestation of spores was observed. A linear negative correlation (r = -0.329) was revealed between the V. destructor and Nosema spp. in honeybees colonies. In general, all the colonies exhibited moderate to severe infection by Nosema spp. spores and 93% of the colonies had V. destructor, which necessitates urgent mitigation measures.Se estudió el nivel de infestación y la asociación ecológica de Nosema spp. y Varroa destructor en abejas. La tasa de infestación (Mn±SD) de V. destructor osciló entre el 1,05 ± 0,64% y el 9,02 ± 4,98%. La prevalencia de Nosema spp., en el estado de Osun, osciló entre baja y semisevera, mientras que en el estado de Ogun variaron de moderados a severos. Sin embargo, en ambos estados se observó infestación grave de esporas. Se reveló correlación lineal negativa (r = -0,329) entre V. destructor y Nosema spp. en las colonias de abejas. En general, todas las colonias presentaban una infección de moderada a grave por esporas de Nosema spp. y el 93% de las colonias tenían V. destructor, lo que requiere medidas urgentes de mitigación
Development of a molecular procedure for the identification of Varroa destructor in southwestern Nigeria
No full textAbstract Varroa mites are a major cause among other factors such as disease, climate change, and other identified pests of the global decline in honeybees’ colonies. The four species of Varroa mites (which have varying destructive capability) are morphologically similar and the current technique of using the body ratio may lead to misidentification. The present study was designed to investigate the species of Varroa mites in Nigeria and the molecular procedure for identifying the mites. The body ratio of the varroa mites ranged between 1.46 ± 0.00 and 1.53 ± 0.03 across the four states studied in southwestern Nigeria, suggestive of Varroa destructor (≥1.4). Molecular identification using universal primers LCO 1490 and HCO 2198 failed to amplify the COX1 gene of the samples. However, a species-specific primer designed by the local team amplified the targeted gene at 230 bp. The results highlighted that V. destructor is the major pest mite in southwestern Nigeria and it can be easily identified using the species-specific primers presented in this study
