7,046 research outputs found

    Ligand binding by r<i>Ab</i>-FAR-1.

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    <p><b>(A)</b><i>Ab</i>-FAR-1. The reverse change of DAUDA emission was observed after the addition of oleic acid to the r<i>Ab</i>-FAR-1+DAUDA complex. The wavelengths of peak emission by DAUDA were different. <b>(B)</b> Fluorescence emission spectra (excitation at 350 nm) of retinol in ethanol alone or after the addition of r<i>Ab</i>-FAR-1. The competitive effect of oleic acid was also found. <b>(C)</b> Fluorescence emission spectra (excitation at 319 nm) of cis-parinaric acid alone or after the addition of r<i>Ab</i>-FAR-1.The effects of the subsequent addition of retinol to the r<i>Ab</i>-FAR-1+cis-parinaric acid and addition of oleic acid solution to the r<i>Ab</i>-FAR-1+cis-parinaric acid+ retinol were examined.</p

    Fluorescent titration lipid binding analysis of r<i>Ab</i>-FAR-1.

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    <p><b>(A)</b> Change in relative fluorescence intensity (excitation at 345 nm) of DAUDA (10 µM) in the presence of increasing concentrations of r<i>Ab</i>-FAR-1. The best fit curve was used to determine the dissociation constant (Kd) for the DAUDA: r<i>Ab</i>-FAR-1 interaction. <b>(B)</b> Change in relative fluorescence intensity (excitation at 350 nm) of 10 µM r<i>Ab</i>-FAR-1 in the presence of increasing concentrations of retinol. The curve was used to derive the equilibrium dissociation constant (Kd) for the retinol: r<i>Ab</i>-FAR-1 interaction. <b>(C)</b> Change in relative fluorescence intensity (excitation at 319 nm) of cis-parinaric acid (10 µM) in the presence of increasing concentrations of r<i>Ab</i>-FAR-1. The best fit curve was used to determine the dissociation constant (Kd) for the cis-parinaric acid: r<i>Ab</i>-FAR-1 interaction.</p

    The vanishing author in computer-generated works: a critical analysis of recent Australian case law

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    Abstract The use of software is ubiquitous in the creation of many copyright works, yet the requirement in copyright law that every work have a human author who engages in independent intellectual effort means that its use may prevent copyright subsistence. Several recent Australian cases have refocused attention on authorship as an essential criterion of copyright subsistence, and these cases suggest that much computer-produced output may be authorless and thus lack copyright protection. This article, the first in a two-part series, analyses how each case deals with the question of authorship of computer-produced works and why the use of software diminishes copyright protection for a significant number of computer-generated works. The article critiques the application of conventional notions of human authorship developed in the pre-computer age to modern productions and suggests alternative approaches to authorship that satisfy both the major objectives of copyright policy and the need to adapt to the computer age. The article argues that, without a broader judicial approach to authorship of computer-generated works, Parliament must remedy the lacuna in protection for these ‘authorless’ works. Possible solutions for reform are suggested. In a forthcoming article, the author comprehensively examines those reform proposals

    Drumheller, AB

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    Notes - A history of the Ursaline order in Drumheller, AB from 1935 to 1985 (2 pages)Drumhelle

    Souvenir of Edmonton, AB

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    Booklet - Souvenir of Edmonton - The Capital City of Alberta. Collection of photographs in a green cover tied with string, Edmonton, AB (48 pages

    Ab initio Calculation of CuSbSe2 in Bulk and Monolayer for Solar Cell and Infrared Optoelectronic Applications

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    Bala Idris, Abdullahi Lawal, Dauda Abubakar, Saddiq Abubakar Dalhatu and Buhari Aminu Balesa The production of efficient, less toxic, and low-cost solar cell devices is still faced with numerous challenges. However, copper antimony selenide (CuSbSe2) appears to be the more promising material due to cost-effectiveness, ease of availability, and less toxicity. Therefore, the exploration of the potential of this composite requires, comprehensive analysis of its structural, electronic, and optical properties. To accomplish this purpose, first-principles calculations employing the development of correction terms for the van der Waals interaction has been implemented in this study. Results obtained from structural properties calculations indicated that the role of van der Waals (vdW) interactions on structural properties of layered materials can be predicted from theoretical bases because results obtained for the lattice parameters using vdW on top of PBE were in good correlation with experimental results. The electronic properties investigations gave  values for the electronic band structures, partial and total densities of states. Indirect band gap was observed for bulk CuSbSe2 with band gap value of 0.83 eV, which was also in agreement with experiment result. By reducing the dimension from bulk to monolayer a direct band gap smaller than that of bulk form was obtained, indicating that CuSbSe2 is suitable for solar cell and near infrared optoelectronic applications. The optical gap of CuSbSe2 in bulk and monolayer structure was found to be 0.83 and 0.21 eV respectively. Therefore, CuSbSe2 can absorb photons, whose energy lies between that of the near infrared to visible light frequency. The study confirmed the importance of van der Waals interaction in predicting, structural, electronic and optical properties of layered materials. Analysis of optical parameters suggested that a device fabricated from these materials can be operated on a wide range of energy scale including solar cells, optical communications and biomedical imaging

    Ab initio pair potentials at metal-ceramic interfaces

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    A systematic approach is proposed to obtain the interfacial interatomic potentials. By inverting ab initio adhesive energy curves for the metal-MgO ceramic interfaces, We derive interfacial potentials between Ag and O2-, Ag and Mg2+, Al and O2-, Al and Mg2+. The interfacial potentials, obtained from this method, demonstrate general features of bondings between metal atoms and ceramic ions

    Perceptions of pre- and post-event impacts of PEI 2014 year-long celebrations

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    This study examines Canadian perceptions of pre- and post-event impacts of the PEI 2014 Celebrations using the 2013 and 2015 surveys of PEI residents and other Canadians conducted by the Centre for Tourism Research. Overall, results indicate that Canadians’ awareness of, knowledge about, and attitudes toward the 1864 Charlottetown Conference and the PEI 2014 Celebrations increased and positively changed over time. The research also suggests that event organizers and/or destination tourism managers need to put greater emphasis on the “socio-cultural aspects” of the event such as community participation and cultural identity, and make an effort to increase tourism volume and value in order to obtain strong support from residents and be successful

    Molecular characterization and functions of fatty acid and retinoid binding protein gene (Ab-far-1) in Aphelenchoides besseyi.

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    Rice white tip nematode, Aphelenchoides besseyi, is a kind of plant parasitic nematodes that cause serious losses in rice and many other crops. Fatty acid and retinoid binding protein (FAR) is a specific protein in nematodes and is related to development, reproduction, infection to the host, and disruption of plant defense reactions, so the inhibition of FAR function is the potential approach to control A. besseyi. The full-length of Ab-far-1 cDNA is 805 bp, including 546 bp of ORF that encodes 181 amino acids. Software analysis revealed that the Ab-FAR-1 was rich in α-helix structure, contained a predicted consensus casein kinase II phosphorylation site and a hydrophobic secretory signal peptide, but did not have glycosylation sites. The Ab-FAR-1 had 52% homology to Gp-FAR-1 protein. The Ab-FAR-1 and Gp-FAR-1 were grouped in the same branch according to the phylogenetic tree. Fluorescence-based ligand binding analysis confirmed that the recombinant Ab-FAR-1 (rAb-FAR-1) bound with the fluorescent analogues 11-((5-dimethylaminonaphthalene-1-sulfonyl) amino) undecannoic acid (DAUDA), cis-parinaric acid and retinol, but the oleic acid would compete with the binding site. Quantitative PCR (qPCR) was used to assess the expression level of Ab-far-1 at different development stages of A. besseyi, the highest expression was found in the females, followed by eggs, juveniles and males. Using in situ hybridization technique, Ab-far-1 mRNA was present in the hypodermis of juveniles and adults, the ovaries of females and the testes of males. When A. besseyi was treated with Ab-far-1 dsRNA for 48 h, the silencing efficiency of Ab-far-1 was the best and the number of nematodes on the carrot was the least. Thus FAR plays important roles in the development and reproduction of nematodes. This study is useful and helpful to figure out a new way to control the plant parasitic nematodes

    Ultra-low-power, class-AB, CMOS four-quadrant current multiplier

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    A class-AB four-quadrant current multiplier constituted by a class-AB current amplifier and a current splitter which can handle input signals in excess of ten times the bias current is presented. The proposed circuit operation is based on the exponential characteristic of BJTs or subthreshold MOSFETs. The multiplier is designed using the latter devices and achieves very low power consumption. Simulation results show that from a 0.65 V supply, the proposed circuit consumes 12.4 nW static power while less than 30 dB total harmonic distortion is achieved for an input modulation index up to 10.Microelectronics & Computer EngineeringElectrical Engineering, Mathematics and Computer Scienc
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