1,172 research outputs found
Angiotensin II induces soluble fms-Like tyrosine kinase-1 release via calcineurin signaling pathway in pregnancy
No full textMaternal endothelial dysfunction in preeclampsia is associated with increased soluble fms-like tyrosine kinase-1 (sFlt-1), a circulating antagonist of vascular endothelial growth factor and placental growth factor. Angiotensin II (Ang II) is a potent vasoconstrictor that increases concomitant with sFlt-1 during pregnancy. Therefore, we speculated that Ang II may promote the expression of sFlt-1 in pregnancy. Here we report that infusion of Ang II significantly increases circulating levels of sFlt-1 in pregnant mice, thereby demonstrating that Ang II is a regulator of sFlt-1 secretion in vivo. Furthermore, Ang II stimulated sFlt-1 production in a dose- and time-dependent manner from human villous explants and cultured trophoblasts but not from endothelial cells, suggesting that trophoblasts are the primary source of sFlt-1 during pregnancy. As expected, Ang II-induced sFlt-1 secretion resulted in the inhibition of endothelial cell migration and in vitro tube formation. In vitro and in vivo studies with losartan, small interfering RNA specific for calcineurin and FK506 demonstrated that Ang II-mediated sFlt-1 release was via Ang II type 1 receptor activation and calcineurin signaling, respectively. These findings reveal a previously unrecognized regulatory role for Ang II on sFlt-1 expression in murine and human pregnancy and suggest that elevated sFlt-1 levels in preeclampsia may be caused by a dysregulation of the local renin/angiotensin system
Supplemental Material - Impact on Breast Cancer Survival by Surgical Facility Type Secondary to the ACOSOG Z0011 Trial
No full textSupplemental Material for Impact on Breast Cancer Survival by Surgical Facility Type Secondary to the ACOSOG Z0011 Trial by Alana Hofmann, Huazhi Liu, Edward Copeland, and Darwin Ang in The American Surgeon.</p
sj-pdf-1-asu-10.1177_00031348211056284 – Supplemental Material for Understanding Preventable Deaths in the Geriatric Trauma Population: Analysis of 3,452,339 Patients From the Center of Medicare and Medicaid Services Database
No full textSupplemental Material, sj-pdf-1-asu-10.1177_00031348211056284 for Understanding Preventable Deaths in the Geriatric Trauma Population: Analysis of 3,452,339 Patients From the Center of Medicare and Medicaid Services Database by Darwin Ang, Kenny Nieto, Mason Sutherland, Megan O’Brien, Huazhi Liu and Adel Elkbuli in The American Surgeon</p
Supplemental Material - Palliative Care in the Trauma and Surgical Critical Care Settings: A Narrative Review
No full textSupplemental Material for Palliative Care in the Trauma and Surgical Critical Care Settings: A Narrative Review by Kevin Newsome, Matthew Sauder, Jeffrey Spardy, Lisa Kodadek, Darwin Ang, Christopher P. Michetti, Tracy Bilski, and Adel Elkbuli in The American Surgeon</p
sj-pdf-1-asu-10.1177_00031348211038588 – Supplemental Material for Operative Confidence Among U.S General Surgery Residents
No full textSupplemental Material, sj-pdf-1-asu-10.1177_00031348211038588 for Operative Confidence Among U.S General Surgery Residents by Adel Elkbuli, Haley Ehrlich, Toria Gargano, Kevin Newsome, Huazhi Liu, Mark McKenney and Darwin Ang in The American Surgeon</p
sj-pdf-1-asu-10.1177_00031348221101510 – Supplemental Material for Laparoscopic Versus Laparotomy Surgical Interventions for Trauma Patients with Single Upper Left Quadrant Penetrating Injuries: Analysis of the American College of Surgeons Trauma Quality Improvement Program Dataset
No full textSupplemental Material, sj-pdf-1-asu-10.1177_00031348221101510 for Laparoscopic Versus Laparotomy Surgical Interventions for Trauma Patients with Single Upper Left Quadrant Penetrating Injuries: Analysis of the American College of Surgeons Trauma Quality Improvement Program Dataset by Adel Elkbuli, Kevin Newsome,Dino Fanfan, Mason Sutherland, Tracy Bilski, Huazhi Liu and Darwin Ang in The American Surgeon</p
sj-pdf-1-asu-10.1177_00031348211049251 – Supplemental Material for Hospital Volume of Emergency General Surgery and its Impact on Inpatient Mortality for Geriatric Patients: Analysis From 3994 Hospitals
No full textSupplemental Material, sj-pdf-1-asu-10.1177_00031348211049251 for Hospital Volume of Emergency General Surgery and its Impact on Inpatient Mortality for Geriatric Patients: Analysis From 3994 Hospitals by Darwin Ang, Jonathan Sugimoto, Winston Richards, Huazhi Liu, Kyle Kinslow, Mark McKenney, Michele Ziglar and Adel Elkbuli in The American Surgeon</p
Supplemental material - Assessment of Outcomes in Laparotomy vs Laparoscopy: A Propensity Score Matched Analysis of Patients Sustaining Single Penetrating Left Upper Quadrant Injury
No full textSupplemental material for Assessment of Outcomes in Laparotomy vs Laparoscopy: A Propensity Score Matched Analysis of Patients Sustaining Single Penetrating Left Upper Quadrant Injury by Adel Elkbuli, Ryan Andrade, Micah Ngatuvai, Areeba Khan, Huazhi Liu, Tracy Bilski, and Darwin Ang in The American Surgeon</p
sj-pdf-1-asu-10.1177_00031348211067993 – Supplemental Material for Evaluation of Citation Inaccuracies in Surgical Literature by Journal Type, Study Design, and Level of Evidence: Towards Safeguarding the Peer-Review Process
No full textSupplemental Material, sj-pdf-1-asu-10.1177_00031348211067993 for Evaluation of Citation Inaccuracies in Surgical Literature by Journal Type, Study Design, and Level of Evidence: Towards Safeguarding the Peer-Review Process by Matthew Sauder, Kevin Newsome, Israel Zagales, Cody Autrey, Snigdha Das, Darwin Ang and Adel Elkbuli in The American Surgeon</p
The need for improved identification and accurate classification of stages 3-5 Chronic Kidney Disease in primary care: retrospective cohort study
No full textBackground Around ten percent of the population have been reported as having Chronic Kidney Disease (CKD), which is associated with increased cardiovascular mortality. Few previous studies have ascertained the chronicity of CKD. In the UK, a payment for performance (P4P) initiative incentivizes CKD (stages 3–5) recognition and management in primary care, but the impact of this has not been assessed. Methods and Findings Using data from 426 primary care practices (population 2,707,130), the age standardised prevalence of stages 3–5 CKD was identified using two consecutive estimated Glomerular Filtration Rates (eGFRs) seven days apart. Additionally the accuracy of practice CKD registers and the relationship between accurate identification of CKD and the achievement of P4P indicators was determined. Between 2005 and 2009, the prevalence of stages 3–5 CKD increased from 0.3% to 3.9%. In 2009, 30,440 patients (1.1% unadjusted) fulfilled biochemical criteria for CKD but were not on a practice CKD register (uncoded CKD) and 60,705 patients (2.2% unadjusted) were included on a practice CKD register but did not fulfil biochemical criteria (miscoded CKD). For patients with confirmed CKD, inclusion in a practice register was associated with increasing age, male sex, diabetes, hypertension, cardiovascular disease and increasing CKD stage (p<0.0001). Uncoded CKD patients compared to miscoded patients were less likely to achieve performance indicators for blood pressure (OR 0.84, 95% CI 0.82–0.86 p<0.001) or recorded albumin-creatinine ratio (OR 0.73, 0.70–0.76, p<0.001). Conclusions The prevalence of stages 3–5 CKD, using two laboratory reported eGFRs, was lower than estimates from previous studies. Clinically significant discrepancies were identified between biochemically defined CKD and appearance on practice registers, with misclassification associated with sub-optimal care for some people with CKD
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